Construct: ORF TRCN0000466206
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010413.1_s317c1
- Derived from:
- ccsbBroadEn_14053
- DNA Barcode:
- CTACACTCGTTGTGTCCGATGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC12A7 (10723)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466206
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10723 | SLC12A7 | solute carrier family 12 me... | NM_006598.3 | 23.4% | 21.3% | (many diffs) |
| 2 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XM_005248231.3 | 23.3% | 21.2% | (many diffs) |
| 3 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XM_011513941.2 | 21.7% | 18.2% | (many diffs) |
| 4 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XM_011513939.3 | 21.1% | 18% | (many diffs) |
| 5 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XM_011513940.2 | 20.9% | 18.1% | (many diffs) |
| 6 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XM_017008958.1 | 17% | 15% | (many diffs) |
| 7 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XM_017008959.1 | 17% | 15% | (many diffs) |
| 8 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XR_925582.2 | 13.9% | (many diffs) | |
| 9 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XR_925580.2 | 13.5% | (many diffs) | |
| 10 | human | 10723 | SLC12A7 | solute carrier family 12 me... | XR_925579.2 | 13.5% | (many diffs) | |
| 11 | mouse | 20499 | Slc12a7 | solute carrier family 12, m... | NM_011390.2 | 20.3% | 19.5% | (many diffs) |
| 12 | mouse | 20499 | Slc12a7 | solute carrier family 12, m... | XM_006517172.3 | 20.3% | 19.4% | (many diffs) |
| 13 | mouse | 20499 | Slc12a7 | solute carrier family 12, m... | XM_006517173.3 | 18.6% | 17.1% | (many diffs) |
| 14 | mouse | 20499 | Slc12a7 | solute carrier family 12, m... | XM_006517174.3 | 17.6% | 16.9% | (many diffs) |
| 15 | mouse | 20499 | Slc12a7 | solute carrier family 12, m... | XM_006517175.2 | 15.5% | 14.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 864
- ORF length:
- 798
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caccaacttc accgtggtgc ccgtggaggc tcacgccgac ggcggcgggg 121 acgagactgc cgagcggacg gaggctccgg gcacccccga gggccccgag cccgagcgcc 181 ccagcccggg agatggaaat ccaagagaaa acagcccatt cctcaacaat gtcgaggtgg 241 aacaagagag cttctttgaa gggaagaaca tggcactttt cgaggaggag atggacagta 301 accccatggt gtcctcgctg ctcaacaagc tggccaacta caccaacctg agccagggcg 361 tggtggagca cgaggaggac gaggagagcc ggcggcggga ggccaaggct ccgcgcatgg 421 gcaccttcat cggcgtctac ctgccgtgcc tgcagaacat cctgggcgtc atcctcttcc 481 tgcgcctgac gtggatcgtg ggggtggctg gtgtcctgga gtccttccTC ATCGTGGCCA 541 TGTGCTGCAC ATGTACAATG CTGACCGCCA TTTCCATGAG TGCGATCGCT ACCAACGGTG 601 TGGTCCCAGC TGGCGGGTCC TACTACATGA TATCGCGCTC GCTGGGACCC GAGTTTGGAG 661 GCGCTGTCGG CCTCTGCTTC TACCTGGGCA CGACGTTTGC AGGGGCCATG TATATTTTGG 721 GGACCATCGA GATTTTTCTG ACAGGCACGG AGGACGGCGT GGGCAGCCTG GGCCTGGGCT 781 TGGCCCAGGG AAACAGACGG CAGACACACC TGTCCCCCAG TGATGCCGCC CAAGCTGCCC 841 ATGGGGCTTT CTACGGAAGT TTCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TACACTCGTT 1021 GTGTCCGATG GTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att