Construct: ORF TRCN0000466264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018365.1_s317c1
- Derived from:
- ccsbBroadEn_01831
- DNA Barcode:
- GACTTCAAATAAATAGCTTCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBA1A (7846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466264
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270399.1 | 100% | 100% | |
| 2 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_006009.4 | 100% | 100% | |
| 3 | human | 10376 | TUBA1B | tubulin alpha 1b | NM_006082.3 | 97.2% | 99.5% | (many diffs) |
| 4 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_032704.5 | 95.9% | 98% | (many diffs) |
| 5 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270400.1 | 92.2% | 92.2% | 0_1ins105 |
| 6 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303115.2 | 88.1% | 90.2% | (many diffs) |
| 7 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303116.2 | 88.1% | 90.2% | (many diffs) |
| 8 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303117.2 | 88.1% | 90.2% | (many diffs) |
| 9 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_006000.3 | 84.4% | 95.7% | (many diffs) |
| 10 | human | 113457 | TUBA3D | tubulin alpha 3d | NM_080386.4 | 84.4% | 97.5% | (many diffs) |
| 11 | human | 7278 | TUBA3C | tubulin alpha 3c | NM_006001.3 | 84.2% | 97.5% | (many diffs) |
| 12 | human | 112714 | TUBA3E | tubulin alpha 3e | NM_207312.3 | 84% | 96.2% | (many diffs) |
| 13 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303114.1 | 82.9% | 84.6% | (many diffs) |
| 14 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_001278552.1 | 81.6% | 92.6% | (many diffs) |
| 15 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_018943.3 | 80.5% | 89.8% | (many diffs) |
| 16 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_001193414.2 | 69.1% | 78.7% | (many diffs) |
| 17 | human | 7277 | TUBA4A | tubulin alpha 4a | XM_017004824.1 | 55.9% | 63.8% | (many diffs) |
| 18 | mouse | 22142 | Tuba1a | tubulin, alpha 1A | NM_011653.2 | 93.6% | 100% | (many diffs) |
| 19 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | NM_011654.2 | 93% | 99.5% | (many diffs) |
| 20 | mouse | 22146 | Tuba1c | tubulin, alpha 1C | NM_009448.4 | 92.4% | 98.2% | (many diffs) |
| 21 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | XM_006520756.1 | 84.7% | 90.7% | (many diffs) |
| 22 | mouse | 22147 | Tuba3b | tubulin, alpha 3B | NM_009449.3 | 84.4% | 97.5% | (many diffs) |
| 23 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_009447.4 | 84% | 95.7% | (many diffs) |
| 24 | mouse | 22144 | Tuba3a | tubulin, alpha 3A | NM_009446.3 | 83.7% | 97.5% | (many diffs) |
| 25 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | XM_006496478.1 | 81.3% | 92.6% | (many diffs) |
| 26 | mouse | 53857 | Tuba8 | tubulin, alpha 8 | NM_017379.2 | 78.6% | 90% | (many diffs) |
| 27 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313724.1 | 55.8% | 63.8% | (many diffs) |
| 28 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313723.1 | 46.4% | 52.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1419
- ORF length:
- 1353
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tgagtgcatc tccatccacg ttggccaggc tggtgtccag attggcaatg 121 cctgctggga gctctactgc ctggaacacg gcatccagcc cgatggccag atgccaagtg 181 acaagaccat tgggggagga gatgattcct tcaacacctt cttcagtgag acgggggctg 241 gcaagcatgt gccccgggca gtgtttgtag acttggaacc cacagtcatt gatgaagttc 301 gcactggcac ctaccgccag ctcttccacc ctgagcaact tatcacaggc aaagaagatg 361 ctgccaataa ctatgcccga gggcactaca ccattggcaa ggagatcatt gacctcgtgt 421 tggaccgaat tcgcaagctg gccgaccagt gcacgggtct ccagggcttc ttggttttcc 481 acagctttgg tgggggaact ggttctgggt tcacctcgct gctcatggaa cgtctctcag 541 ttgattatgg caagaagtcc aagctggagt tctctattta cccggcgccc caggtttcca 601 cagctgtagt tgagccctac aactccatcc tcaccaccca caccaccctg gagcactctg 661 attgtgcctt catggtagac aatgaggcca tctatgacat ctgtcgtaga aacctcgata 721 ttgagcgtcc aacctatact aacctgaata ggttaatagg tcaaattgtg tcctccatca 781 ctgcttccct gagatttgat ggagccctga atgttgacct gacagaattc cagaccaacc 841 tggtgcccta tccccgcatc cacttccctc tggccacata tgcccctgtc atctctgctg 901 agaaagccta ccatgaacag ctttctgtag cagagatcac caatgcttgc tttgagccag 961 ccaaccagat ggtgaaatgt gaccctcgcc atggtaaata catggcttgc tgcctgttgt 1021 accgtggtga cgtggttccc aaagatgtca atgctgccat tgccaccatc aagaccaagc 1081 gtaccatcca gtttgtggat tgGTGCCCCA CTGGCTTCAA GGTTGGCATC AACTACCAGC 1141 CTCCCACTGT GGTGCCTGGT GGAGACCTGG CCAAGGTACA GAGAGCTGTG TGCATGCTGA 1201 GCAACACCAC AGCCATTGCT GAGGCCTGGG CTCGCCTGGA CCACAAGTTT GACCTGATGT 1261 ATGCCAAACG TGCCTTTGTT CACTGGTACG TTGGGGAGGG GATGGAGGAA GGTGAGTTTT 1321 CAGAGGCCCG TGAGGACATG GCTGCCCTTG AGAAGGATTA TGAGGAGGTT GGTGTGGATT 1381 CTGTTGAAGG AGAGGGTGAG GAAGAAGGAG AGGAATACTA CCCAACTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGAGACTTC AAATAAATAG CTTCACTCAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt