Construct: ORF TRCN0000466264

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018365.1_s317c1
Derived from:
ccsbBroadEn_01831
DNA Barcode:
GACTTCAAATAAATAGCTTCACTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TUBA1A (7846)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466264
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7846 TUBA1A tubulin alpha 1a NM_001270399.1 100% 100%
2 human 7846 TUBA1A tubulin alpha 1a NM_006009.4 100% 100%
3 human 10376 TUBA1B tubulin alpha 1b NM_006082.3 97.2% 99.5% (many diffs)
4 human 84790 TUBA1C tubulin alpha 1c NM_032704.5 95.9% 98% (many diffs)
5 human 7846 TUBA1A tubulin alpha 1a NM_001270400.1 92.2% 92.2% 0_1ins105
6 human 84790 TUBA1C tubulin alpha 1c NM_001303115.2 88.1% 90.2% (many diffs)
7 human 84790 TUBA1C tubulin alpha 1c NM_001303116.2 88.1% 90.2% (many diffs)
8 human 84790 TUBA1C tubulin alpha 1c NM_001303117.2 88.1% 90.2% (many diffs)
9 human 7277 TUBA4A tubulin alpha 4a NM_006000.3 84.4% 95.7% (many diffs)
10 human 113457 TUBA3D tubulin alpha 3d NM_080386.4 84.4% 97.5% (many diffs)
11 human 7278 TUBA3C tubulin alpha 3c NM_006001.3 84.2% 97.5% (many diffs)
12 human 112714 TUBA3E tubulin alpha 3e NM_207312.3 84% 96.2% (many diffs)
13 human 84790 TUBA1C tubulin alpha 1c NM_001303114.1 82.9% 84.6% (many diffs)
14 human 7277 TUBA4A tubulin alpha 4a NM_001278552.1 81.6% 92.6% (many diffs)
15 human 51807 TUBA8 tubulin alpha 8 NM_018943.3 80.5% 89.8% (many diffs)
16 human 51807 TUBA8 tubulin alpha 8 NM_001193414.2 69.1% 78.7% (many diffs)
17 human 7277 TUBA4A tubulin alpha 4a XM_017004824.1 55.9% 63.8% (many diffs)
18 mouse 22142 Tuba1a tubulin, alpha 1A NM_011653.2 93.6% 100% (many diffs)
19 mouse 22143 Tuba1b tubulin, alpha 1B NM_011654.2 93% 99.5% (many diffs)
20 mouse 22146 Tuba1c tubulin, alpha 1C NM_009448.4 92.4% 98.2% (many diffs)
21 mouse 22143 Tuba1b tubulin, alpha 1B XM_006520756.1 84.7% 90.7% (many diffs)
22 mouse 22147 Tuba3b tubulin, alpha 3B NM_009449.3 84.4% 97.5% (many diffs)
23 mouse 22145 Tuba4a tubulin, alpha 4A NM_009447.4 84% 95.7% (many diffs)
24 mouse 22144 Tuba3a tubulin, alpha 3A NM_009446.3 83.7% 97.5% (many diffs)
25 mouse 22145 Tuba4a tubulin, alpha 4A XM_006496478.1 81.3% 92.6% (many diffs)
26 mouse 53857 Tuba8 tubulin, alpha 8 NM_017379.2 78.6% 90% (many diffs)
27 mouse 22145 Tuba4a tubulin, alpha 4A NM_001313724.1 55.8% 63.8% (many diffs)
28 mouse 22145 Tuba4a tubulin, alpha 4A NM_001313723.1 46.4% 52.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1419
ORF length:
1353
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcg tgagtgcatc tccatccacg ttggccaggc tggtgtccag attggcaatg
121cctgctggga gctctactgc ctggaacacg gcatccagcc cgatggccag atgccaagtg
181acaagaccat tgggggagga gatgattcct tcaacacctt cttcagtgag acgggggctg
241gcaagcatgt gccccgggca gtgtttgtag acttggaacc cacagtcatt gatgaagttc
301gcactggcac ctaccgccag ctcttccacc ctgagcaact tatcacaggc aaagaagatg
361ctgccaataa ctatgcccga gggcactaca ccattggcaa ggagatcatt gacctcgtgt
421tggaccgaat tcgcaagctg gccgaccagt gcacgggtct ccagggcttc ttggttttcc
481acagctttgg tgggggaact ggttctgggt tcacctcgct gctcatggaa cgtctctcag
541ttgattatgg caagaagtcc aagctggagt tctctattta cccggcgccc caggtttcca
601cagctgtagt tgagccctac aactccatcc tcaccaccca caccaccctg gagcactctg
661attgtgcctt catggtagac aatgaggcca tctatgacat ctgtcgtaga aacctcgata
721ttgagcgtcc aacctatact aacctgaata ggttaatagg tcaaattgtg tcctccatca
781ctgcttccct gagatttgat ggagccctga atgttgacct gacagaattc cagaccaacc
841tggtgcccta tccccgcatc cacttccctc tggccacata tgcccctgtc atctctgctg
901agaaagccta ccatgaacag ctttctgtag cagagatcac caatgcttgc tttgagccag
961ccaaccagat ggtgaaatgt gaccctcgcc atggtaaata catggcttgc tgcctgttgt
1021accgtggtga cgtggttccc aaagatgtca atgctgccat tgccaccatc aagaccaagc
1081gtaccatcca gtttgtggat tgGTGCCCCA CTGGCTTCAA GGTTGGCATC AACTACCAGC
1141CTCCCACTGT GGTGCCTGGT GGAGACCTGG CCAAGGTACA GAGAGCTGTG TGCATGCTGA
1201GCAACACCAC AGCCATTGCT GAGGCCTGGG CTCGCCTGGA CCACAAGTTT GACCTGATGT
1261ATGCCAAACG TGCCTTTGTT CACTGGTACG TTGGGGAGGG GATGGAGGAA GGTGAGTTTT
1321CAGAGGCCCG TGAGGACATG GCTGCCCTTG AGAAGGATTA TGAGGAGGTT GGTGTGGATT
1381CTGTTGAAGG AGAGGGTGAG GAAGAAGGAG AGGAATACTA CCCAACTTTC TTGTACAAAG
1441TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1501GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1561ACGAGACTTC AAATAAATAG CTTCACTCAC GCGTTAAGTC gacaatcaac ctctggatta
1621caaaatttgt gaaagatt

Download FASTA (ORF) (Full)