Construct: ORF TRCN0000466644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004643.1_s317c1
- Derived from:
- ccsbBroadEn_15690
- DNA Barcode:
- ACGGATCAATCGAGATTTTAAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP1M2 (10053)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10053 | AP1M2 | adaptor related protein com... | NM_005498.5 | 99.7% | 99.5% | 615A>G;1258C>T;1264A>C |
| 2 | human | 10053 | AP1M2 | adaptor related protein com... | NM_001300887.2 | 99.2% | 98.8% | (many diffs) |
| 3 | human | 10053 | AP1M2 | adaptor related protein com... | XM_024451303.1 | 83.8% | 83.4% | (many diffs) |
| 4 | human | 10053 | AP1M2 | adaptor related protein com... | XM_024451304.1 | 83.5% | 82.9% | (many diffs) |
| 5 | mouse | 11768 | Ap1m2 | adaptor protein complex AP-... | NM_009678.2 | 87% | 96.6% | (many diffs) |
| 6 | mouse | 11768 | Ap1m2 | adaptor protein complex AP-... | NM_001110300.1 | 86.5% | 96% | (many diffs) |
| 7 | mouse | 11768 | Ap1m2 | adaptor protein complex AP-... | XM_006509950.3 | 79.6% | 88.4% | (many diffs) |
| 8 | mouse | 11768 | Ap1m2 | adaptor protein complex AP-... | XM_006509949.3 | 79.2% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1335
- ORF length:
- 1269
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgcctcggct gtcttcattc tggacgttaa gggcaagcca ttgatcagcc 121 gcaactacaa gggcgatgtg gccatgagca agattgagca cttcatgcct ttgctggtac 181 agcgggagga ggaaggcgcc ctggccccgc tgctgagcca cggccaggtc cacttcctat 241 ggatcaaaca cagcaacctc tacttggtgg ccaccacatc gaagaatgcc aatgcctccc 301 tggtgtactc cttcctgtat aagacaatag aggtattctg cgaatacttc aaggagctgg 361 aggaggagag catccgggac aactttgtca tcgtctacga gttgctggac gagctcatgg 421 actttggctt cccgcagacc accgacagca agatcctgca ggagtacatc actcagcaga 481 gcaacaagct ggagacgggc aagtcacggg tgccacccac tgtcaccaac gctgtgtcct 541 ggcgctccga gggtatcaag tataagaaga acgaggtctt cattgatgtc atagagtctg 601 tcaacctgct ggtcaatgcc aacggcagcg tccttctgag cgaaatcgtc ggtaccatca 661 agctcaaggt gtttctgtcg ggaatgccag agctgcggct gggcctcaat gaccgcgtgc 721 tcttcgagct cactggccgc agcaagaaca aatcagtaga gctggaggat gtaaaattcc 781 accagtgcgt gcggctctct cgctttgaca acgaccgcac catctccttc atcccgcctg 841 atggtgactt tgagctcatg tcataccgcc tcagcaccca ggtcaagcca ctgatctgga 901 ttgagtctgt cattgagaag ttctcccaca gccgcgtgga gatcatggtc aaggccaagg 961 ggCAGTTTAA GAAACAGTCA GTGGCCAACG GTGTGGAGAT ATCTGTGCCT GTACCCAGCG 1021 ATGCCGACTC CCCCAGATTC AAGACCAGTG TGGGCAGCGC CAAGTATGTG CCGGAGAGAA 1081 ACGTCGTGAT TTGGAGTATT AAGTCTTTCC CGGGGGGCAA GGAGTACTTG ATGCGAGCCC 1141 ACTTTGGCCT CCCCAGTGTG GAAAAGGAAG AGGTGGAGGG CCGGCCCCCC ATCGGGGTCA 1201 AGTTTGAGAT CCCCTACTTC ACCGTCTCTG GGATCCAGGT CCGATACATG AAGATCATTG 1261 AGAAAAGTGG TTACCAGGCC CTGCCCTGGG TTCGCTACAT CACCCAGAGT GGCGATTACC 1321 AATTTCGTCC CAGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1381 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1441 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACGGATCAAT CGAGATTTTA 1501 AATAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt