Construct: ORF TRCN0000466827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011081.1_s317c1
- Derived from:
- ccsbBroadEn_02826
- DNA Barcode:
- TCCGGAGTGCATATCCTCCCTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC7A11 (23657)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23657 | SLC7A11 | solute carrier family 7 mem... | NM_014331.4 | 99.9% | 100% | 1443G>A |
| 2 | human | 23657 | SLC7A11 | solute carrier family 7 mem... | XM_011531802.3 | 96.9% | 95.6% | (many diffs) |
| 3 | human | 23657 | SLC7A11 | solute carrier family 7 mem... | XR_001741190.2 | 15.4% | (many diffs) | |
| 4 | human | 23657 | SLC7A11 | solute carrier family 7 mem... | XR_001741191.2 | 14.2% | (many diffs) | |
| 5 | mouse | 26570 | Slc7a11 | solute carrier family 7 (ca... | NM_011990.2 | 87.2% | 89% | (many diffs) |
| 6 | mouse | 26570 | Slc7a11 | solute carrier family 7 (ca... | XM_006500776.3 | 83.7% | 85.4% | (many diffs) |
| 7 | mouse | 26570 | Slc7a11 | solute carrier family 7 (ca... | XM_006500777.3 | 79.7% | 65.8% | (many diffs) |
| 8 | mouse | 26570 | Slc7a11 | solute carrier family 7 (ca... | XM_006500778.3 | 77.5% | 73.2% | (many diffs) |
| 9 | mouse | 26570 | Slc7a11 | solute carrier family 7 (ca... | XM_017319590.1 | 56.9% | 58.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cagaaagcct gttgtgtcca ccatctccaa aggaggttac ctgcagggaa 121 atgttaacgg gaggctgcct tccctgggca acaaggagcc acctgggcag gagaaagtgc 181 agctgaagag gaaagtcact ttactgaggg gagtctccat tatcattggc accatcattg 241 gagcaggaat cttcatctct cctaagggcg tgctccagaa cacgggcagc gtgggcatgt 301 ctctgaccat ctggacggtg tgtggggtcc tgtcactatt tggagctttg tcttatgctg 361 aattgggaac aactataaag aaatctggag gtcattacac atatattttg gaagtctttg 421 gtccattacc agcttttgta cgagtctggg tggaactcct cataatacgc cctgcagcta 481 ctgctgtgat atccctggca tttggacgct acattctgga accatttttt attcaatgtg 541 aaatccctga acttgcgatc aagctcatta cagctgtggg cataactgta gtgatggtcc 601 taaatagcat gagtgtcagc tggagcgccc ggatccagat tttcttaacc ttttgcaagc 661 tcacagcaat tctgataatt atagtccctg gagttatgca gctaattaaa ggtcaaacgc 721 agaactttaa agacgccttt tcaggaagag attcaagtat tacgcggttg ccactggctt 781 tttattatgg aatgtatgca tatgctggct ggttttacct caactttgtt actgaagaag 841 tagaaaaccc tgaaaaaacc attccccttg caatatgtat atccatggcc attgtcacca 901 ttggctatgt gctgacaaat gtggcctact ttacgaccat taatgctgag gagctgctgc 961 tttcaaatgc agtggcagtg accttttctg agcggctact gggaaatttc tcattagcag 1021 ttccgatctt tgttgccctc tcctgctttg gctccatgaa cggtggtgtg tttgctgtct 1081 ccaggttatt ctatgttgcg tctcgagagg gtcaccTTCC AGAAATCCTC TCCATGATTC 1141 ATGTCCGCAA GCACACTCCT CTACCAGCTG TTATTGTTTT GCACCCTTTG ACAATGATAA 1201 TGCTCTTCTC TGGAGACCTC GACAGTCTTT TGAATTTCCT CAGTTTTGCC AGGTGGCTTT 1261 TTATTGGGCT GGCAGTTGCT GGGCTGATTT ATCTTCGATA CAAATGCCCA GATATGCATC 1321 GTCCTTTCAA GGTGCCACTG TTCATCCCAG CTTTGTTTTC CTTCACATGC CTCTTCATGG 1381 TTGCCCTTTC CCTCTATTCG GACCCATTTA GTACAGGGAT TGGCTTCGTC ATCACTCTGA 1441 CTGGAGTCCC TGCGTATTAT CTCTTTATTA TATGGGACAA GAAACCCAGG TGGTTTAGAA 1501 TAATGTCAGA GAAAATAACC AGAACATTAC AAATAATACT GGAAGTTGTA CCAGAAGAAG 1561 ATAAGTTATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCCGGA GTGCATATCC TCCCTTACAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt