Construct: ORF TRCN0000467160
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004490.1_s317c1
- Derived from:
- BRDN0000394419
- DNA Barcode:
- CAGGGACTCGGCCGAGGACCGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA0753 (9851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467160
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9851 | KIAA0753 | KIAA0753 | NM_014804.3 | 99.6% | 99.3% | (many diffs) |
| 2 | human | 9851 | KIAA0753 | KIAA0753 | XM_011524090.3 | 97.4% | 97.1% | (many diffs) |
| 3 | human | 9851 | KIAA0753 | KIAA0753 | XM_011524091.2 | 97.4% | 97.1% | (many diffs) |
| 4 | human | 9851 | KIAA0753 | KIAA0753 | XM_011524095.2 | 97.4% | 97.1% | (many diffs) |
| 5 | human | 9851 | KIAA0753 | KIAA0753 | XM_017025455.2 | 97.4% | 97.1% | (many diffs) |
| 6 | human | 9851 | KIAA0753 | KIAA0753 | XM_011524096.2 | 93.8% | 93.4% | (many diffs) |
| 7 | human | 9851 | KIAA0753 | KIAA0753 | NM_001351225.2 | 68.8% | 68.5% | (many diffs) |
| 8 | human | 9851 | KIAA0753 | KIAA0753 | XM_006721612.2 | 68.8% | 68.5% | (many diffs) |
| 9 | human | 9851 | KIAA0753 | KIAA0753 | XM_011524098.2 | 67.3% | 67% | (many diffs) |
| 10 | human | 9851 | KIAA0753 | KIAA0753 | XM_011524099.2 | 67.3% | 67% | (many diffs) |
| 11 | human | 9851 | KIAA0753 | KIAA0753 | XM_011524100.3 | 67.3% | 67% | (many diffs) |
| 12 | human | 9851 | KIAA0753 | KIAA0753 | XR_001752707.2 | 66.1% | (many diffs) | |
| 13 | human | 9851 | KIAA0753 | KIAA0753 | XR_934126.2 | 65.1% | (many diffs) | |
| 14 | human | 9851 | KIAA0753 | KIAA0753 | XR_001752709.2 | 63.2% | (many diffs) | |
| 15 | human | 9851 | KIAA0753 | KIAA0753 | XR_001752708.2 | 62.3% | (many diffs) | |
| 16 | human | 9851 | KIAA0753 | KIAA0753 | NR_147086.2 | 54.9% | (many diffs) | |
| 17 | human | 9851 | KIAA0753 | KIAA0753 | NR_147087.2 | 53.5% | (many diffs) | |
| 18 | human | 9851 | KIAA0753 | KIAA0753 | NR_147088.2 | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2970
- ORF length:
- 2901
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaccaggc cagcctgcac caacctgtgt tcatctagca cctaggaccc 121 aacttgatgg gaggagcgac cccaaagtac ttcagaccca gaaccagctg cagtttaata 181 ggaatgttcc tacacattca agcaacttgg cgatccgata ttcttgccca catgccatta 241 gaattgaaaa actgaagcac tcatacaatg aatcatacca ttgtaaagat gcagattgta 301 gagttggtcc tgacctgggc agttctgttt cattttccgt catatcccaa gagagactta 361 gctatgctgt ccacctagcc agaagagatg tgaaacgaag acaatttgaa aaacatataa 421 aagaacatca tctcagaagt cagcctcaaa gctctcagaa gtgtggacat actaagtata 481 aaatacccga ccacagggtg gaaaggaagg aatcaaagag tcaagcagcc tgtcagtgta 541 gccaccagcc atccaaagta gaaatctcca gctccggtgc caaagtatac ctttactcat 601 ctcatccagg ccagtcagat cttactgtgc caaattcgcc acccacccat gatccgggac 661 ttcagcctca tcccaggata ggtgaccaca aaaacataag tgaacagaaa agcctgctag 721 aagtccagcg actccagaaa gaactgagca gttgtatcca caaaattgaa gaggtaacta 781 aaaaagatag actagaagaa gctttggatc cagatgaaga acgtcgaatc cgtatcagga 841 gacaggagca agctgctcgc tctgcccgaa tgctctacgt cctccagcag caggtaaaag 901 aaatccagga agaattggat aaattgagtc cacataaaat taaacacact aagaagtcat 961 gggcaatgtc taagctggcg gctgcccatc gaggagccat tcgggcctta cagatgtttg 1021 tcactcagtt tactgaccga ggggagcatc cacttcctgc tcggtgtaag gaactgggca 1081 gccttattcg ccagctttca ctttgttctg tcaagctgga tgcagaccct tctgttcctg 1141 atgtggttat agatattctg caacaaattg aggctttgga atctctcctg gaaaagaaac 1201 tgtcaccaaa aaaggtgaaa aaatgtttca gtgaaattcg gagcagattt cctatcggta 1261 gccaaaaggc cttggagaga tggccaagta catcaccaaa gggtgagagg aggcccctca 1321 cagcaaagga cacattccca caggaaacaa gtcgaccttc tgtagcaaag cagcttcttg 1381 ccgataagta tcagcccaat acggagcttc cggagaccca gaggttgcag agtgagcttg 1441 atgtattaga tgcggatata gttccggaag aaggaccatt tattctagac caaagtgcaa 1501 gcttcaaaga cgaggtgtta gccgtggcaa aaacaaaagc agggaaaaag aagcctgtga 1561 ctgagaatgt gccgtttagg aagaaagata ctctggcgcc agcaagacag caaggactcc 1621 gcaaagctga aagaggtaga caaagccaac ctcacagtaa aagcagagtg cagcagacaa 1681 cagtttcatc cagattaaaa atgaaccggc agcctgtgaa agaccgcaag gcaccatgga 1741 tacccccaaa ccccacatcc ccaccagcgt ctcctaaatg tgccgcatgg ctaaaggtga 1801 aaactagccc cagagatgcc acaaaagagc ctctccagca agaagatcct caagaggaaa 1861 gtcacctgac aggtgctgtt gagcatgaag cagccaggct cgcttggctt gatgctgaaa 1921 cttccaaaag attgaaggaa ctagaagagt taaaagccaa ggaaattgac agcatgcaaa 1981 aacagaggct tgattggctt gatgctgaaa cttctagaag aacaaaggaa ctgaatgagc 2041 tcaaagctga agaaatgtat agactccaac aattgagtgt ttctgccacc cacttagcag 2101 acaaggtaga ggaggcagtt ctggatcgtt tgaagcctct cttggtcaag gcccagagag 2161 tcaattctac tacagaagca aatattcatt tgaaagatgg ctcgtcagta aacacagcgc 2221 caggccagcc tgcacaggag gttgcagctg ttgattttga atccaacaac attcgtcagc 2281 ttgatgattt tttggaagat tgtgccagtg agctctgggc tgtgactcat gctaagatct 2341 tggggtctga aaccttagcc accgttgagg acagcaagga tagcccagat ctggagatca 2401 tgatgcgccg aatggaagag atggaaaaat accaggagtc tgttcgtcaa agatataata 2461 aaatcgcata tgctgatcct cgactttgga tgcaggaaga aaacaatgac caaaaaatct 2521 cagcaataag tgaaaagcct ctatctcctc atccaatcag aaTCACGAAG ACAGTGGATC 2581 GCAAGGATCC AGCCGTGAAC ATCATGTTAG AAAGGCCCTG TAATGGCAAT TCCCTGGATG 2641 AAAGTGTGGG AACAGAGGAA GGATCAGAGA AAAGAGAGGC CCCTCTTCTC TCCCTAGCCG 2701 AAGATTCTCA ACAGAAAGAA GGCCGAGCTC CCCTCTTTGT CCCACCGGGT ATGCGGCACA 2761 GCATCGGTGA CTACTGTAGT CGTTTTGAGC AGTACCTTCG GATCATATCT CATGAGGCTG 2821 TAGGCTCCTT CAACCCGTGG CTGATAGCTG AAAGCTTTTC AGAAGAGCTG GTAGATGAAG 2881 CTCTGGGTGC TGTGGCTGCT GAACTTCAGG ATATGTGCGA AGATTATGCA GAAGCTGTGT 2941 TCACCTCAGA ATTCTTAGAG GCTGCTACAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 3001 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3061 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAGGG 3121 ACTCGGCCGA GGACCGGCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3181 tgaaagatt