Construct: ORF TRCN0000468185

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000532.1_s317c1
Derived from:
ccsbBroadEn_09825
DNA Barcode:
TTTTAGCTTTGTCAGTAAAATCGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KHDRBS2 (202559)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468185
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 202559 KHDRBS2 KH RNA binding domain conta... NM_152688.4 99.8% 99.7% 876T>C;976C>A
2 human 202559 KHDRBS2 KH RNA binding domain conta... NM_001350622.1 95.1% 95% 811_861del;927T>C;1027C>A
3 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010344.1 85.7% 85.6% 336_337ins147;729T>C;829C>A
4 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010345.1 85.5% 85.1% 876T>C;893_894insTGTGCCTGA;897_898ins141
5 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010343.1 85.5% 85.6% (many diffs)
6 human 202559 KHDRBS2 KH RNA binding domain conta... XM_011535515.2 84.3% 79.2% (many diffs)
7 human 202559 KHDRBS2 KH RNA binding domain conta... XM_011535517.1 83.7% 78.9% (many diffs)
8 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010335.1 82.9% 68.6% (many diffs)
9 human 202559 KHDRBS2 KH RNA binding domain conta... XM_011535519.1 82% 77.5% (many diffs)
10 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010342.1 81.6% 81.1% (many diffs)
11 human 202559 KHDRBS2 KH RNA binding domain conta... XM_011535516.1 81.5% 81.6% (many diffs)
12 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010341.1 81.5% 81.6% (many diffs)
13 human 202559 KHDRBS2 KH RNA binding domain conta... XM_011535520.1 80% 79% (many diffs)
14 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010334.1 76.7% 63.5% (many diffs)
15 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010336.1 75.5% 70.9% 811_986del;1052T>C;1101_1102ins122
16 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010339.1 73.4% 71.1% (many diffs)
17 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010340.1 73.4% 71.1% (many diffs)
18 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010347.1 71.7% 71.6% 0_1ins294;582T>C;682C>A
19 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010338.1 65.2% 52.5% (many diffs)
20 human 202559 KHDRBS2 KH RNA binding domain conta... XR_001743201.1 64.3% (many diffs)
21 human 202559 KHDRBS2 KH RNA binding domain conta... XR_001743200.1 62.6% (many diffs)
22 human 202559 KHDRBS2 KH RNA binding domain conta... NR_146873.2 58.8% (many diffs)
23 human 202559 KHDRBS2 KH RNA binding domain conta... XR_001743204.1 56.2% (many diffs)
24 human 202559 KHDRBS2 KH RNA binding domain conta... XR_001743206.1 55.4% (many diffs)
25 human 202559 KHDRBS2 KH RNA binding domain conta... NR_146874.2 55.2% (many diffs)
26 human 202559 KHDRBS2 KH RNA binding domain conta... XR_001743205.1 54.5% (many diffs)
27 human 202559 KHDRBS2 KH RNA binding domain conta... NR_146871.2 53.1% (many diffs)
28 human 202559 KHDRBS2 KH RNA binding domain conta... NR_146870.1 51.4% (many diffs)
29 human 202559 KHDRBS2 KH RNA binding domain conta... XR_001743202.1 51.1% (many diffs)
30 human 202559 KHDRBS2 KH RNA binding domain conta... XR_001743212.1 49.6% (many diffs)
31 human 202559 KHDRBS2 KH RNA binding domain conta... NR_146872.1 43.4% (many diffs)
32 human 202559 KHDRBS2 KH RNA binding domain conta... XM_017010346.1 42.9% 28.3% (many diffs)
33 human 202559 KHDRBS2 KH RNA binding domain conta... XR_001743199.1 23.8% (many diffs)
34 mouse 170771 Khdrbs2 KH domain containing, RNA b... NM_133235.3 91.2% 95.1% (many diffs)
35 mouse 170771 Khdrbs2 KH domain containing, RNA b... XR_373242.3 45.1% (many diffs)
36 mouse 170771 Khdrbs2 KH domain containing, RNA b... XR_001783141.1 43.6% (many diffs)
37 mouse 170771 Khdrbs2 KH domain containing, RNA b... XR_001783140.1 42.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1113
ORF length:
1047
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga agaggagaaa tatttgcctg agctgatggc agagaaagat agcctggatc
121catcttttgt gcatgcgtcg cgccttttgg cagaagaaat tgaaaagttt caaggttctg
181atggaaaaaa ggaagacgaa gaaaagaagt atcttgatgt catcagcaac aaaaacataa
241agctctcaga aagagtactg attcctgtca agcagtatcc aaagttcaat tttgtgggga
301aattgcttgg accaagagga aactccttga agaggctaca ggaagaaaca ggtgctaaaa
361tgtctatcct gggcaaagga tcaatgagag ataaagctaa ggaagaagaa ctaaggaaga
421gtggggaagc caaatatgcc cacttgagtg atgagcttca tgtattaatt gaagtgtttg
481ctccacctgg ggaagcttat tcacgtatga gtcatgcatt ggaagagatt aaaaaattcc
541tggttcctga ctacaatgat gaaattcgtc aggaacaact acgtgaatta tcttacttaa
601atggctcaga ggactctggt cgtggcagag gtattagagg cagagggatc agaatagctc
661ccacagctcc ttcaaggggc cgtgggggtg ccattcctcc TCCCCCACCA CCTGGACGAG
721GTGTTCTCAC CCCTCGGGGA AGCACTGTAA CCCGTGGAGC GCTTCCAGTG CCACCTGTAG
781CAAGAGGTGT CCCTACCCCT CGAGCCCGGG GGGCACCAAC AGTGCCAGGA TACAGGGCAC
841CTCCTCCTCC AGCCCATGAA GCTTATGAAG AATATGGTTA TGATGATGGC TACGGGGGTG
901AATATGATGA CCAGACCTAT GAGACTTATG ATAACAGCTA CGCGACCCAA ACACAAAGTG
961TGCCTGAATA CTATGACTAC GGTCATGGAG TAAGTGAGGA TGCCTATGAC AGCTACGCAC
1021CAGAAGAATG GGCCACAACC AGCTCTAGCT TGAAGGCACC ACCGCAAAGG TCAGCCAGAG
1081GGGGATACAG GGAACACCCC TATGGTAGAT ATTGCCCAAC TTTCTTGTAC AAAGTGGTTG
1141ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1201GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT
1261TTAGCTTTGT CAGTAAAATC GAACGCGTTA AGTCgacaat caacctctgg attacaaaat
1321ttgtgaaaga tt

Download FASTA (ORF) (Full)