Construct: ORF TRCN0000468200
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008065.1_s317c1
- Derived from:
- ccsbBroadEn_07193
- DNA Barcode:
- TTACGAATGGATTTTAGCATAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST7 (7982)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468200
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7982 | ST7 | suppression of tumorigenici... | NM_021908.3 | 99.9% | 99.8% | 1739C>T |
| 2 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369604.1 | 96% | 95.8% | 640_641ins69;1670C>T |
| 3 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369598.1 | 95.6% | 94% | (many diffs) |
| 4 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369601.1 | 94.8% | 93% | (many diffs) |
| 5 | human | 7982 | ST7 | suppression of tumorigenici... | NM_018412.4 | 91.7% | 90.1% | (many diffs) |
| 6 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369599.1 | 87.9% | 85.5% | (many diffs) |
| 7 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369602.1 | 87.6% | 85.6% | (many diffs) |
| 8 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369606.1 | 87.1% | 85.3% | (many diffs) |
| 9 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369600.1 | 84% | 81.6% | (many diffs) |
| 10 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369603.1 | 83.7% | 81.7% | (many diffs) |
| 11 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369607.1 | 83.2% | 81.4% | (many diffs) |
| 12 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161422.1 | 77.6% | (many diffs) | |
| 13 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161420.1 | 76.6% | (many diffs) | |
| 14 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161423.1 | 74.3% | (many diffs) | |
| 15 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161421.1 | 73.5% | (many diffs) | |
| 16 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161418.1 | 68.1% | (many diffs) | |
| 17 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161419.1 | 65.3% | (many diffs) | |
| 18 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289624.1 | 88.1% | 93.3% | (many diffs) |
| 19 | mouse | 64213 | St7 | suppression of tumorigenici... | XM_011241074.1 | 88.1% | 93.3% | (many diffs) |
| 20 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289625.1 | 87.3% | 92.3% | (many diffs) |
| 21 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289626.1 | 84.2% | 89.4% | (many diffs) |
| 22 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_022332.3 | 81.4% | 85.5% | (many diffs) |
| 23 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289627.1 | 81.1% | 85.5% | (many diffs) |
| 24 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001083315.2 | 77.5% | 81.6% | (many diffs) |
| 25 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289629.1 | 77.2% | 81.6% | (many diffs) |
| 26 | mouse | 64213 | St7 | suppression of tumorigenici... | XM_011241076.2 | 62.8% | 66% | (many diffs) |
| 27 | mouse | 64213 | St7 | suppression of tumorigenici... | XM_011241075.2 | 59.9% | 65.7% | (many diffs) |
| 28 | mouse | 64213 | St7 | suppression of tumorigenici... | XR_377291.3 | 52.5% | (many diffs) | |
| 29 | mouse | 64213 | St7 | suppression of tumorigenici... | XR_868763.2 | 52% | (many diffs) | |
| 30 | mouse | 64213 | St7 | suppression of tumorigenici... | XR_377290.3 | 51.2% | (many diffs) | |
| 31 | mouse | 64213 | St7 | suppression of tumorigenici... | XR_868764.2 | 36.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1821
- ORF length:
- 1755
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag 61 ttggcatggc tgaagcggcc acgggctttc tggagcagct caagtcctgc atagtttggt 121 cttggacgta tctgtggacc gtgtggttct tcatcgtgct attcctggtc tacatcctgc 181 gggtgccttt gaaaatcaac gacaacttga gcacagtgag catgtttttg aacacattaa 241 caccgaagtt ctacgtggcc ctaacaggca cttcctcact aatatcaggg cttattttga 301 tatttgaatg gtggtatttt cgcaaatacg gaacttcatt cattgaacaa gtctcagtaa 361 gccacttgcg cccccttctg ggaggggttg acaacaactc ttccaacaat tctaattcca 421 gtaacgggga ctcagattcc aataggcaaa gtgtctcaga atgcaaagta tggcgaaatc 481 cactaaattt atttaggggt gctgaataca atcggtatac ttgggtgaca ggacgagagc 541 ctcttactta ctatgacatg aatctctctg cccaagacca ccagacattc tttacttgtg 601 actcggacca tctgcgtccc gcagatgcaa taatgcagaa agcctggaga gagagaaacc 661 cccaagctag gatttctgca gctcatgaag ccttggagat aaatgaaatt aggtccagag 721 ttgaagttcc cctaattgct tcctctacca tctgggagat aaaattgtta ccaaagtgtg 781 caactgctta tattctcttg gctgaagagg aagcaacaac cattgctgaa gcagaaaaat 841 tatttaagca ggccctgaag gctggagatg gctgttaccg acgctctcag cagctacaac 901 atcatggatc ccagtatgaa gcccaacata gacgagacac caatgtcttg gtgtacatca 961 aaagaaggct agcaatgtgt gccagaagac tcgggaggac cagggaagca gtgaaaatga 1021 tgagagattt aatgaaggag ttcccccttc tgagtatgtt caatatccat gaaaaccttt 1081 tagaagccct tctggaacta caagcatatg ctgatgttca ggcagtctta gcaaagtatg 1141 atgatataag cttaccaaag tcagcaacaa tatgctacac agctgctttg ctcaaagcaa 1201 gagctgtctc tgacaaattc tctcctgagg ctgcatctcg gcgggggctg agcacagcag 1261 agatgaatgc agtagaggcc attcatagag ctgtggaatt caatcctcat gtgccaaaat 1321 acctactaga aatgaaaagc ttaatcctac ccccagaaca tatcctgaag agaggagaca 1381 gtgaagcaat agcatatgca ttctttcatc ttgcacactg gaagagagtg gaaggggctt 1441 tgaaTCTTTT GCATTGTACG TGGGAAGGCA CTTTTCGGAT GATCCCTTAT CCCTTGGAAA 1501 AGGGGCACCT ATTTTATCCT TACCCAATCT GTACAGAAAC AGCAGACCGA GAGCTGCTTC 1561 CATCTTTCCA TGAAGTCTCA GTTTACCCAA AGAAGGAGCT TCCCTTCTTT ATTCTCTTTA 1621 CTGCTGGATT ATGTTCCTTC ACAGCCATGC TGGCCCTCCT GACACATCAG TTCCCGGAAC 1681 TTATGGGGGT CTTCGCAAAA GCTATGATTG ACATTTTCTG CTCGGCAGAG TTCAGGGACT 1741 GGAATTGCAA GAGTATTTTC ATGCGTGTTG AAGATGAACT GGAAATCCCT CCGGCACCTC 1801 AATTTCAACA TTTCCAAAAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1861 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1921 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTAC GAATGGATTT 1981 TAGCATAAAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt