Transcript: Mouse XR_377291.3

PREDICTED: Mus musculus suppression of tumorigenicity 7 (St7), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
St7 (64213)
Length:
2880
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_377291.3
NBCI Gene record:
St7 (64213)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_377291.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000249439 TTACCAAAGTCGGCGACAATA pLKO_005 1840 3UTR 100% 13.200 18.480 N St7 n/a
2 TRCN0000183751 CGGAATATAATCGGTACACTT pLKO.1 1190 3UTR 100% 4.950 6.930 N St7 n/a
3 TRCN0000249440 CCTTGGAGATAAACGAAATTA pLKO_005 1379 3UTR 100% 15.000 10.500 N St7 n/a
4 TRCN0000215948 CACCAATGTCTTAGTCTATAT pLKO.1 1626 3UTR 100% 13.200 9.240 N St7 n/a
5 TRCN0000249438 CACCAATGTCTTAGTCTATAT pLKO_005 1626 3UTR 100% 13.200 9.240 N St7 n/a
6 TRCN0000257898 CTCTCATGGACCGCAAGAAAG pLKO_005 2678 3UTR 100% 10.800 7.560 N St7 n/a
7 TRCN0000179803 CCATACCCAATCTGTACAGAA pLKO.1 2342 3UTR 100% 4.950 3.465 N St7 n/a
8 TRCN0000038061 GCTGATGTTCAGGCAGTCTTA pLKO.1 1798 3UTR 100% 4.950 3.465 N ST7 n/a
9 TRCN0000286522 GCTGATGTTCAGGCAGTCTTA pLKO_005 1798 3UTR 100% 4.950 3.465 N ST7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_377291.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07193 pDONR223 100% 52.5% None (many diffs) n/a
2 ccsbBroad304_07193 pLX_304 0% 52.5% V5 (many diffs) n/a
3 TRCN0000468200 TTACGAATGGATTTTAGCATAAAT pLX_317 21.8% 52.5% V5 (many diffs) n/a
4 ccsbBroadEn_11249 pDONR223 100% 50.9% None (many diffs) n/a
5 ccsbBroad304_11249 pLX_304 0% 50.9% V5 (many diffs) n/a
6 TRCN0000479746 TGGCTCGCTACCTCCTCGCAGCAC pLX_317 21.8% 50.9% V5 (many diffs) n/a
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