Construct: ORF TRCN0000468567

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013201.1_s317c1
Derived from:
ccsbBroadEn_01895
DNA Barcode:
AACCATAGCTAACCCTGCACACAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
H2AC17 (8336)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468567
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8336 H2AC17 H2A clustered histone 17 NM_003514.2 100% 100%
2 human 8330 H2AC15 H2A clustered histone 15 NM_003510.2 90.2% 100% (many diffs)
3 human 8332 H2AC16 H2A clustered histone 16 NM_003511.2 90% 100% (many diffs)
4 human 8969 H2AC11 H2A clustered histone 11 NM_021064.4 88.7% 100% (many diffs)
5 human 8329 H2AC13 H2A clustered histone 13 NM_003509.2 88.4% 100% (many diffs)
6 human 3013 H2AC7 H2A clustered histone 7 NM_021065.3 87.9% 99.2% (many diffs)
7 human 8334 H2AC6 H2A clustered histone 6 NM_003512.3 87.1% 98.4% (many diffs)
8 human 8331 H2AC14 H2A clustered histone 14 NM_021066.2 86.6% 97.6% (many diffs)
9 human 85235 H2AC12 H2A clustered histone 12 NM_080596.2 86.1% 98.4% (many diffs)
10 human 723790 H2AC19 H2A clustered histone 19 NM_001040874.1 85.6% 98.4% (many diffs)
11 human 8337 H2AC18 H2A clustered histone 18 NM_003516.2 85.6% 98.4% (many diffs)
12 human 92815 H2AW H2A.W histone NM_033445.2 85.1% 97.6% (many diffs)
13 human 8338 H2AC20 H2A clustered histone 20 NM_003517.2 84.6% 96.9% (many diffs)
14 human 3012 H2AC8 H2A clustered histone 8 NM_021052.2 84.1% 98.4% (many diffs)
15 human 8335 H2AC4 H2A clustered histone 4 NM_003513.2 83.2% 98.4% (many diffs)
16 human 55766 H2AJ H2A.J histone NM_177925.3 82% 95.3% (many diffs)
17 human 317772 H2AC21 H2A clustered histone 21 NM_175065.2 78.5% 94.6% (many diffs)
18 human 3014 H2AX H2A.X variant histone NM_002105.2 75.5% 83.9% (many diffs)
19 mouse 319191 Hist1h2ai histone cluster 1, H2ai NM_178182.2 88.2% 98.4% (many diffs)
20 mouse 665433 Hist1h2ao histone cluster 1, H2ao NM_001177544.2 87.9% 98.4% (many diffs)
21 mouse 319170 Hist1h2an histone cluster 1, H2an NM_178184.2 87.9% 98.4% (many diffs)
22 mouse 319171 Hist1h2ap histone cluster 1, H2ap NM_178185.2 87.9% 98.4% (many diffs)
23 mouse 319167 Hist1h2ag histone cluster 1, H2ag NM_178186.3 87.9% 98.4% (many diffs)
24 mouse 319166 Hist1h2ae histone cluster 1, H2ae NM_178187.4 87.9% 98.4% (many diffs)
25 mouse 319164 Hist1h2ac histone cluster 1, H2ac NM_178189.5 87.9% 98.4% (many diffs)
26 mouse 15267 Hist2h2aa1 histone cluster 2, H2aa1 NM_013549.2 87.6% 98.4% (many diffs)
27 mouse 319165 Hist1h2ad histone cluster 1, H2ad NM_178188.4 87.6% 98.4% (many diffs)
28 mouse 319192 Hist2h2aa2 histone cluster 2, H2aa2 NM_178212.3 87.6% 98.4% (many diffs)
29 mouse 319172 Hist1h2ab histone cluster 1, H2ab NM_175660.3 87.4% 98.4% (many diffs)
30 mouse 319173 Hist1h2af histone cluster 1, H2af NM_175661.2 87.1% 97.6% (many diffs)
31 mouse 319176 Hist2h2ac histone cluster 2, H2ac NM_175662.2 87.1% 96.9% (many diffs)
32 mouse 319168 Hist1h2ah histone cluster 1, H2ah NM_175659.2 86.9% 96.9% (many diffs)
33 mouse 319169 Hist1h2ak histone cluster 1, H2ak NM_178183.2 86.4% 97.6% (many diffs)
34 mouse 232440 H2afj H2A histone family, member J NM_177688.4 82.8% 94.6% (many diffs)
35 mouse 319162 Hist3h2a histone cluster 3, H2a NM_178218.4 82.5% 97.6% (many diffs)
36 mouse 319163 Hist1h2aa histone cluster 1, H2aa NM_175658.2 80.1% 88.4% (many diffs)
37 mouse 621893 Hist2h2ab histone cluster 2, H2ab NM_178213.4 78.1% 93.8% (many diffs)
38 mouse 15270 H2afx H2A histone family, member X NM_010436.2 76.7% 83.2% (many diffs)
39 mouse 667728 Hist1h2al histone cluster 1, H2al NR_132443.1 11% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
90
ORF end:
480
ORF length:
390
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTGGACG TGGCAAGCAG GGCGGCAAGG
121CTCGCGCCAA GGCCAAAACC CGCTCCTCTA GAGCTGGGCT CCAATTTCCT GTAGGACGAG
181TGCACCGCCT GCTCCGCAAG GGCAACTACG CTGAGCGGGT CGGGGCCGGC GCGCCGGTTT
241ACCTGGCGGC GGTGCTGGAG TACCTAACTG CCGAGATCCT GGAGCTGGCG GGCAACGCAG
301CCCGCGACAA CAAAAAGACC CGCATCATCC CGCGCCACTT GCAGCTGGCC ATCCGCAACG
361ACGAGGAGCT CAACAAGCTG CTTGGTAAAG TTACCATCGC TCAGGGCGGT GTTCTGCCTA
421ACATCCAGGC CGTACTGCTC CCCAAGAAGA CTGAGAGCCA CCACAAAGCT AAGGGCAAGT
481ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
541GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
601TTTATATATC TTGTGGAAAG GACGAAACCA TAGCTAACCC TGCACACACA CGCGTTAAGT
661Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)