Construct: ORF TRCN0000469289
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003051.1_s317c1
- Derived from:
- ccsbBroadEn_00717
- DNA Barcode:
- TCCAAGCCCTCATTGGAGCTGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AX (3014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469289
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3014 | H2AX | H2A.X variant histone | NM_002105.2 | 100% | 100% | |
| 2 | human | 92815 | H2AW | H2A.W histone | NM_033445.2 | 78.3% | 83.9% | (many diffs) |
| 3 | human | 8332 | H2AC16 | H2A clustered histone 16 | NM_003511.2 | 77.4% | 83.9% | (many diffs) |
| 4 | human | 8330 | H2AC15 | H2A clustered histone 15 | NM_003510.2 | 76.9% | 83.9% | (many diffs) |
| 5 | human | 3013 | H2AC7 | H2A clustered histone 7 | NM_021065.3 | 76.9% | 83.2% | (many diffs) |
| 6 | human | 8329 | H2AC13 | H2A clustered histone 13 | NM_003509.2 | 76.5% | 83.9% | (many diffs) |
| 7 | human | 8969 | H2AC11 | H2A clustered histone 11 | NM_021064.4 | 76.5% | 83.9% | (many diffs) |
| 8 | human | 8334 | H2AC6 | H2A clustered histone 6 | NM_003512.3 | 76.2% | 84.6% | (many diffs) |
| 9 | human | 85235 | H2AC12 | H2A clustered histone 12 | NM_080596.2 | 76% | 82.5% | (many diffs) |
| 10 | human | 723790 | H2AC19 | H2A clustered histone 19 | NM_001040874.1 | 75.8% | 83.9% | (many diffs) |
| 11 | human | 8337 | H2AC18 | H2A clustered histone 18 | NM_003516.2 | 75.8% | 83.9% | (many diffs) |
| 12 | human | 8338 | H2AC20 | H2A clustered histone 20 | NM_003517.2 | 75.6% | 83.9% | (many diffs) |
| 13 | human | 55766 | H2AJ | H2A.J histone | NM_177925.3 | 75.6% | 83.2% | (many diffs) |
| 14 | human | 8336 | H2AC17 | H2A clustered histone 17 | NM_003514.2 | 75.5% | 83.9% | (many diffs) |
| 15 | human | 317772 | H2AC21 | H2A clustered histone 21 | NM_175065.2 | 74.8% | 83.9% | (many diffs) |
| 16 | human | 8335 | H2AC4 | H2A clustered histone 4 | NM_003513.2 | 74% | 83.2% | (many diffs) |
| 17 | human | 8331 | H2AC14 | H2A clustered histone 14 | NM_021066.2 | 73.9% | 82.5% | (many diffs) |
| 18 | human | 3012 | H2AC8 | H2A clustered histone 8 | NM_021052.2 | 73.4% | 83.2% | (many diffs) |
| 19 | mouse | 15270 | H2afx | H2A histone family, member X | NM_010436.2 | 92.5% | 97.2% | (many diffs) |
| 20 | mouse | 319170 | Hist1h2an | histone cluster 1, H2an | NM_178184.2 | 81.2% | 83.2% | (many diffs) |
| 21 | mouse | 665433 | Hist1h2ao | histone cluster 1, H2ao | NM_001177544.2 | 81.1% | 83.2% | (many diffs) |
| 22 | mouse | 319191 | Hist1h2ai | histone cluster 1, H2ai | NM_178182.2 | 81.1% | 83.2% | (many diffs) |
| 23 | mouse | 319171 | Hist1h2ap | histone cluster 1, H2ap | NM_178185.2 | 81.1% | 83.2% | (many diffs) |
| 24 | mouse | 319167 | Hist1h2ag | histone cluster 1, H2ag | NM_178186.3 | 80.9% | 83.2% | (many diffs) |
| 25 | mouse | 319165 | Hist1h2ad | histone cluster 1, H2ad | NM_178188.4 | 80.9% | 83.2% | (many diffs) |
| 26 | mouse | 319173 | Hist1h2af | histone cluster 1, H2af | NM_175661.2 | 80.7% | 83.9% | (many diffs) |
| 27 | mouse | 15267 | Hist2h2aa1 | histone cluster 2, H2aa1 | NM_013549.2 | 80.6% | 83.9% | (many diffs) |
| 28 | mouse | 319192 | Hist2h2aa2 | histone cluster 2, H2aa2 | NM_178212.3 | 80.6% | 83.9% | (many diffs) |
| 29 | mouse | 319168 | Hist1h2ah | histone cluster 1, H2ah | NM_175659.2 | 80.4% | 81.8% | (many diffs) |
| 30 | mouse | 319166 | Hist1h2ae | histone cluster 1, H2ae | NM_178187.4 | 80.2% | 83.2% | (many diffs) |
| 31 | mouse | 319172 | Hist1h2ab | histone cluster 1, H2ab | NM_175660.3 | 80.2% | 83.2% | (many diffs) |
| 32 | mouse | 319164 | Hist1h2ac | histone cluster 1, H2ac | NM_178189.5 | 80.2% | 83.2% | (many diffs) |
| 33 | mouse | 319176 | Hist2h2ac | histone cluster 2, H2ac | NM_175662.2 | 79.9% | 83.9% | (many diffs) |
| 34 | mouse | 319169 | Hist1h2ak | histone cluster 1, H2ak | NM_178183.2 | 79.3% | 83.2% | (many diffs) |
| 35 | mouse | 232440 | H2afj | H2A histone family, member J | NM_177688.4 | 79.2% | 83.3% | (many diffs) |
| 36 | mouse | 319162 | Hist3h2a | histone cluster 3, H2a | NM_178218.4 | 76.7% | 83.9% | (many diffs) |
| 37 | mouse | 621893 | Hist2h2ab | histone cluster 2, H2ab | NM_178213.4 | 75.5% | 83.2% | (many diffs) |
| 38 | mouse | 319163 | Hist1h2aa | histone cluster 1, H2aa | NM_175658.2 | 72.3% | 78.3% | (many diffs) |
| 39 | mouse | 667728 | Hist1h2al | histone cluster 1, H2al | NR_132443.1 | 12.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 495
- ORF length:
- 429
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC GGGCCGCGGC AAGACTGGCG GCAAGGCCCG CGCCAAGGCC AAGTCGCGCT 121 CGTCGCGCGC CGGCCTCCAG TTCCCAGTGG GCCGTGTACA CCGGCTGCTG CGGAAGGGCC 181 ACTACGCCGA GCGCGTTGGC GCCGGCGCGC CAGTGTACCT GGCGGCAGTG CTGGAGTACC 241 TCACCGCTGA GATCCTGGAG CTGGCGGGCA ATGCGGCCCG CGACAACAAG AAGACGCGAA 301 TCATCCCCCG CCACCTGCAG CTGGCCATCC GCAACGACGA GGAGCTCAAC AAGCTGCTGG 361 GCGGCGTGAC GATCGCCCAG GGAGGCGTCC TGCCCAACAT CCAGGCCGTG CTGCTGCCCA 421 AGAAGACCAG CGCCACCGTG GGGCCGAAGG CGCCCTCGGG CGGCAAGAAG GCCACCCAGG 481 CCTCCCAGGA GTACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 541 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 601 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCCAAGCCCT CATTGGAGCT 661 GGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt