Construct: ORF TRCN0000470328

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008409.1_s317c1
Derived from:
ccsbBroadEn_10967
DNA Barcode:
CTTGCATGAATTTATTTTATTCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MIA2 (4253)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470328
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001247989.1 99.8% 99.5% (many diffs)
2 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354151.2 99.2% 98.6% (many diffs)
3 human 4253 MIA2 MIA SH3 domain ER export fa... NM_005930.4 99.2% 98.8% (many diffs)
4 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021314.1 98.7% 98.4% (many diffs)
5 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354150.2 97.5% 95.6% (many diffs)
6 human 4253 MIA2 MIA SH3 domain ER export fa... XM_011536778.2 97.2% 96% (many diffs)
7 human 4253 MIA2 MIA SH3 domain ER export fa... NM_203354.3 97.2% 96.3% (many diffs)
8 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021315.2 97% 95.1% (many diffs)
9 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021316.1 96.7% 95.8% (many diffs)
10 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354157.2 96% 95.6% (many diffs)
11 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354137.1 95.7% 95.5% (many diffs)
12 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354138.1 95.7% 95.5% (many diffs)
13 human 4253 MIA2 MIA SH3 domain ER export fa... NM_203356.2 95.7% 95.5% (many diffs)
14 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021317.2 95.2% 95.1% (many diffs)
15 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021318.1 95.2% 95.1% (many diffs)
16 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021319.1 95.2% 95.1% (many diffs)
17 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021330.1 95.2% 95.1% (many diffs)
18 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354154.2 94% 93% (many diffs)
19 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354152.2 93.9% 93.3% (many diffs)
20 human 4253 MIA2 MIA SH3 domain ER export fa... NM_203355.3 93.9% 93.5% (many diffs)
21 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354147.1 92.5% 92.3% (many diffs)
22 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354146.2 91.9% 91% (many diffs)
23 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354153.2 90.6% 90.3% (many diffs)
24 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354148.1 90.5% 89.7% (many diffs)
25 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001247988.1 90.3% 90.2% (many diffs)
26 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354139.1 90.3% 90.2% (many diffs)
27 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001247990.2 90% 89.8% 0_1ins240;390G>C;1870A>G
28 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354140.2 89.4% 89.2% (many diffs)
29 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354141.2 89.4% 89.2% (many diffs)
30 human 4253 MIA2 MIA SH3 domain ER export fa... XM_011536785.2 89.4% 89.2% (many diffs)
31 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021323.1 89% 88.8% (many diffs)
32 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021324.2 89% 88.8% (many diffs)
33 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449598.1 89% 88.8% (many diffs)
34 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354155.2 88.7% 87.7% (many diffs)
35 human 100142659 CTAGE8 CTAGE family member 8 NM_001278507.1 88% 80.6% (many diffs)
36 human 643854 CTAGE9 CTAGE family member 9 NM_001145659.1 88% 80.1% (many diffs)
37 human 100128553 CTAGE4 CTAGE family member 4 NM_198495.2 87.9% 80.1% (many diffs)
38 human 441294 CTAGE15 CTAGE family member 15 NM_001008747.2 87.8% 80.3% (many diffs)
39 human 340307 CTAGE6 CTAGE family member 6 NM_178561.5 87.6% 80.1% (many diffs)
40 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354149.1 87.1% 87% (many diffs)
41 human 64693 CTAGE1 cutaneous T cell lymphoma-a... NM_172241.3 86.3% 81.4% (many diffs)
42 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354156.2 86.1% 86% (many diffs)
43 human 112268382 LOC112268382 cTAGE family member 4-like XR_002959124.1 84.1% (many diffs)
44 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354142.1 84% 83.9% (many diffs)
45 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354143.1 84% 83.9% (many diffs)
46 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354144.2 84% 83.9% (many diffs)
47 human 4253 MIA2 MIA SH3 domain ER export fa... XM_017021327.1 84% 83.9% (many diffs)
48 human 647288 CTAGE11P CTAGE family member 11, pse... NR_027466.1 82.2% (many diffs)
49 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001354145.2 78.9% 78.1% (many diffs)
50 human 119437 CTAGE7P CTAGE family member 7, pseu... NR_044994.1 78.7% (many diffs)
51 human 220429 CTAGE10P CTAGE family member 10, pse... NR_003268.3 75% (many diffs)
52 human 4253 MIA2 MIA SH3 domain ER export fa... NR_148722.2 65.4% (many diffs)
53 human 4253 MIA2 MIA SH3 domain ER export fa... NR_148721.1 63.5% (many diffs)
54 human 4253 MIA2 MIA SH3 domain ER export fa... NR_148723.2 62.4% (many diffs)
55 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449594.1 56.6% 55.5% (many diffs)
56 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449593.1 56.4% 55.1% (many diffs)
57 human 4253 MIA2 MIA SH3 domain ER export fa... NM_001329214.3 56.3% 55.1% (many diffs)
58 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449592.1 56.2% 55.1% (many diffs)
59 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449595.1 53.2% 52.1% (many diffs)
60 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449596.1 53.2% 52.1% (many diffs)
61 human 4253 MIA2 MIA SH3 domain ER export fa... XR_002957555.1 40.2% (many diffs)
62 human 4253 MIA2 MIA SH3 domain ER export fa... XM_024449597.1 40.2% 38.4% (many diffs)
63 human 4253 MIA2 MIA SH3 domain ER export fa... XR_002957554.1 24.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2493
ORF length:
2427
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggagcccggg gctacccctc aaccggattt ggggctgctc ctggaggagc
121tacgcagggt tgtggcagca ctgcctgaag gtatgagacc agattctaat ctttatggtt
181ttccatggga attggtgata tgtgcagctg ttgttggatt ttttgctgtt ctcttttttt
241tgtggagaag ttttagatcg gttaggagtc ggctttatgt gggacgagag aaaaagcttg
301ctctaatgct ttctggacta attgaagaaa aaagtaaact acttgaaaaa tttagccttg
361ttcaaaaaga gtatgaaggc tatgaagtag agtcatcttt aaaggatgcc agctttgaga
421aggaggcaac agaagcacaa agtttggagg tagaaaatca aatggcaacc tgtgaaaagc
481tgaacaggtc caattctgaa cttgaggatg aaatactctg tctagaaaaa gagttaaaag
541aagagaaatc caaacattct gaacaagatg aattgatggc ggatatttca aaaaggatac
601agtctctaga agatgagtca aaatccctca aatcacaagt agctgaagcc aaaatgacct
661tcaagatatt tcaaatgaat gaagaacgac tgaacatagc aataaaagat gctttgaatg
721aaaattctca acttcaggaa agccagaaac agcttttgca agaagctgaa gtatggaaag
781aacaagtgag tgaacttaat aaacagaaag taacatttga agactccaaa gtacatgcag
841aacaagttct aaatgataaa gaaagtcaca tcaagactct gactgaacgc ttgttaaaga
901tgaaagattg ggctgctatg cttggagaag acataacgga tgatgataac ttggaattag
961aaatgaacag tgaatcggaa aatggtgctt acttagataa tcctccaaaa ggagctttga
1021agaaactgat tcatgctgct aagttaaatg cttctttaaa aaccttagaa ggagaaagaa
1081accaaattta tattcagttg tctgaagttg ataaaacaaa ggaagagctt acagagcata
1141ttaaaaatct tcagactgaa caagcatctt tgcagtcaga aaacacacat tttgaaaatg
1201agaatcagaa gcttcaacag aaacttaaag taatgactga attatatcaa gaaaatgaaa
1261tgaaactcca caggaaatta acagtagagg aaaattatcg gttagagaaa gaagagaaac
1321tttctaaagt agatgaaaag atcagccatg ccactgaaga gctggagacc tatagaaagc
1381gagccaaaga tcttgaagaa gaattggaga gaactattca ttcttatcaa gggcagatta
1441tttcccatga gaaaaaagca catgataatt ggttggcagc tcggaatgct gaaagaaacc
1501tcaatgattt aaggaaagaa aatgctcaca acagacaaaa attaactgaa acagagctta
1561aatttgaact tttagaaaaa gatccttatg cactcgatgt tccaaataca gcatttggca
1621gagagcattc cccatatggt ccctcaccat tgggttggcc ttcatctgaa acaagagctt
1681ttctctctcc tccaactttg ttggagggtc cactcagact ctcacctttg cttccagggg
1741gaggaggaag aggctcacga ggcccaggga atcctctgga ccatcagatt accaatgaaa
1801gaggagaatc aagctgtgat aggttaaccg atcctcatag ggctccctct gacactgggt
1861ctctgtcacc tccatgggac caggaccgta ggatgatgtt tcctccgcca ggacaatcat
1921atcctgattc agcccttcct ccacaaaggc aagacagatt ttgttctaat tctggtagac
1981tgtctggacc agcagaactc agaagtttta atatgccttc tttggataaa atggatgggt
2041caatgccttc agaaatggaa tccagtagaa atgataccaa agatgatctt ggtaatttaa
2101atgtgcctga ttcatctctc cctgctgaaa atgaagccac tggccctggc tttgttcctc
2161caccTCTTGC TCCAGTCAGA GGTCCATTGT TTCCAGTGGA TGCAAGAGGC CCATTCTTGA
2221GAAGAGGACC TCCTTTCCCC CCACCTCCTC CAGGAGCCAT GTTTGGAGCT TCTCGAGATT
2281ATTTTCCACC AGGGGATTTC CCAGGTCCAC CACCTGCTCC ATTTGCAATG AGAAATGTCT
2341ATCCACCGAG GGGTTTTCCT CCTTACCTTC CCCCAAGACC TGGATTTTTC CCCCCACCCC
2401CACATTCTGA AGGTAGAAGT GAGTTCCCCT CAGGTTTGAT TCCACCTTCA AATGAGCCTG
2461CTACTGAACA TCCAGAACCA CAGCAAGAAA CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG
2521ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2581GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT
2641TGCATGAATT TATTTTATTC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat
2701ttgtgaaaga tt

Download FASTA (ORF) (Full)