Transcript: Human NM_001354142.1

Homo sapiens MIA SH3 domain ER export factor 2 (MIA2), transcript variant 15, mRNA.

Source:
NCBI, updated 2019-07-10
Taxon:
Homo sapiens (human)
Gene:
MIA2 (4253)
Length:
3272
CDS:
298..2343

Additional Resources:

NCBI RefSeq record:
NM_001354142.1
NBCI Gene record:
MIA2 (4253)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001354142.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000159453 GCCCAAAGCAACAAATTAATA pLKO.1 2902 3UTR 100% 15.000 12.000 N MIA2 n/a
2 TRCN0000159155 GCACGGTTTCTTTATTGCTTT pLKO.1 3022 3UTR 100% 4.950 3.960 N MIA2 n/a
3 TRCN0000160064 CCAATAACTTTGAGGTGCAAA pLKO.1 2803 3UTR 100% 4.950 3.465 N MIA2 n/a
4 TRCN0000165720 CCTCAGGTTTGATTCCACCTT pLKO.1 2276 CDS 100% 2.640 1.848 N MIA2 n/a
5 TRCN0000161286 GCTCTAATGCTTTCTGGACTA pLKO.1 292 5UTR 100% 4.050 2.430 N MIA2 n/a
6 TRCN0000162381 CAAGGGCAGATTATTTCCCAT pLKO.1 1405 CDS 100% 2.640 1.584 N MIA2 n/a
7 TRCN0000337222 AGAGCTTACAGAGCATATTAA pLKO_005 1101 CDS 100% 15.000 7.500 Y CTAGE9 n/a
8 TRCN0000337161 ATGAATTGATGGCGGATATTT pLKO_005 545 CDS 100% 15.000 7.500 Y CTAGE9 n/a
9 TRCN0000159554 CCAAAGATGATCTTGGTAATT pLKO.1 1925 CDS 100% 13.200 6.600 Y MIA2 n/a
10 TRCN0000161026 GAAGAGCTTACAGAGCATATT pLKO.1 1099 CDS 100% 13.200 6.600 Y CTAGE4 n/a
11 TRCN0000147704 GATGAATTGATGGCGGATATT pLKO.1 544 CDS 100% 13.200 6.600 Y CTAGE6 n/a
12 TRCN0000160201 CCAAAGATCTTGAAGAAGAAT pLKO.1 1361 CDS 100% 5.625 2.813 Y CTAGE4 n/a
13 TRCN0000160970 GACCATCAGATTACCAATGAA pLKO.1 1627 CDS 100% 5.625 2.813 Y CTAGE4 n/a
14 TRCN0000005400 GCTGAAAGAAACCTCAATGAT pLKO.1 1465 CDS 100% 5.625 2.813 Y CTAGE1 n/a
15 TRCN0000158680 GCTTTGAAGAAACTGATTCAT pLKO.1 991 CDS 100% 5.625 2.813 Y MIA2 n/a
16 TRCN0000146679 CAATGCCTTCAGAAATGGAAT pLKO.1 1889 CDS 100% 4.950 2.475 Y CTAGE6 n/a
17 TRCN0000158611 CAGATTATTTCCCATGAGAAA pLKO.1 1411 CDS 100% 4.950 2.475 Y MIA2 n/a
18 TRCN0000166046 CCACAGCAAGAAACCTGACAA pLKO.1 2326 CDS 100% 4.950 2.475 Y CTAGE4 n/a
19 TRCN0000005399 CGGATGATGATAACTTGGAAT pLKO.1 914 CDS 100% 4.950 2.475 Y CTAGE1 n/a
20 TRCN0000158647 CGGATGATGATAACTTGGAAT pLKO.1 914 CDS 100% 4.950 2.475 Y MIA2 n/a
21 TRCN0000158825 GCAGATTATTTCCCATGAGAA pLKO.1 1410 CDS 100% 4.950 2.475 Y MIA2 n/a
22 TRCN0000161860 GCCAAAGATCTTGAAGAAGAA pLKO.1 1360 CDS 100% 4.950 2.475 Y CTAGE4 n/a
23 TRCN0000005402 GCTATGAAGTAGAGTCATCTT pLKO.1 371 CDS 100% 4.950 2.475 Y CTAGE1 n/a
24 TRCN0000164371 CAAGATGAATTGATGGCGGAT pLKO.1 541 CDS 100% 2.160 1.080 Y CTAGE15 n/a
25 TRCN0000174282 CAGTTTAAGTAACTGCTGTTA pLKO.1 2390 3UTR 100% 0.495 0.248 Y n/a
26 TRCN0000148442 CCAGGACAATCATATCCTGAT pLKO.1 1756 CDS 100% 0.405 0.203 Y CTAGE6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001354142.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10968 pDONR223 100% 93.4% 93.4% None 143_144insGGTAGAAAATCAAAT;1299_1300ins129 n/a
2 ccsbBroad304_10968 pLX_304 0% 93.4% 93.4% V5 143_144insGGTAGAAAATCAAAT;1299_1300ins129 n/a
3 TRCN0000475416 ATAGTTAGCGCAGAAAAGATACGG pLX_317 11.5% 93.4% 93.4% V5 143_144insGGTAGAAAATCAAAT;1299_1300ins129 n/a
4 ccsbBroadEn_10967 pDONR223 100% 84% 83.9% None (many diffs) n/a
5 ccsbBroad304_10967 pLX_304 0% 84% 83.9% V5 (many diffs) n/a
6 TRCN0000470328 CTTGCATGAATTTATTTTATTCTT pLX_317 13.8% 84% 83.9% V5 (many diffs) n/a
7 ccsbBroadEn_03960 pDONR223 100% 79.2% 74.8% None (many diffs) n/a
8 ccsbBroad304_03960 pLX_304 0% 79.2% 74.8% V5 (many diffs) n/a
9 TRCN0000469836 CGAATATCTAATGCTCGTTTGACG pLX_317 17.1% 79.2% 74.8% V5 (many diffs) n/a
10 ccsbBroadEn_13596 pDONR223 100% 79.1% 72.1% None (many diffs) n/a
11 ccsbBroad304_13596 pLX_304 0% 79.1% 72.1% V5 (many diffs) n/a
12 ccsbBroadEn_13076 pDONR223 100% 78.2% 73.6% None (many diffs) n/a
13 ccsbBroad304_13076 pLX_304 0% 78.2% 73.6% V5 (many diffs) n/a
14 TRCN0000481377 AACTAGAGAATGGGATCGGTCGAA pLX_317 19.7% 78.2% 73.6% V5 (many diffs) n/a
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