Construct: ORF TRCN0000470627
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006164.1_s317c1
- Derived from:
- ccsbBroadEn_13896
- DNA Barcode:
- AGGGTCAACTTGCCCGAATTCAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ATXN3 (4287)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470627
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4287 | ATXN3 | ataxin 3 | NM_004993.6 | 97.2% | 95.6% | (many diffs) |
| 2 | human | 4287 | ATXN3 | ataxin 3 | NM_001127696.2 | 93.2% | 91.6% | (many diffs) |
| 3 | human | 4287 | ATXN3 | ataxin 3 | NM_001127697.2 | 83.5% | 81.8% | (many diffs) |
| 4 | human | 4287 | ATXN3 | ataxin 3 | NM_030660.5 | 82.5% | 80.8% | (many diffs) |
| 5 | human | 4287 | ATXN3 | ataxin 3 | NM_001164781.1 | 76.9% | 74.8% | (many diffs) |
| 6 | human | 4287 | ATXN3 | ataxin 3 | NM_001164779.2 | 63.6% | 61.3% | (many diffs) |
| 7 | human | 4287 | ATXN3 | ataxin 3 | NM_001164780.2 | 49% | 47.2% | (many diffs) |
| 8 | human | 4287 | ATXN3 | ataxin 3 | NM_001164778.2 | 41.5% | 36.6% | (many diffs) |
| 9 | human | 4287 | ATXN3 | ataxin 3 | NM_001164776.2 | 25.4% | 22.2% | (many diffs) |
| 10 | human | 4287 | ATXN3 | ataxin 3 | NM_001164774.2 | 21.6% | 17.6% | (many diffs) |
| 11 | human | 4287 | ATXN3 | ataxin 3 | NR_028459.2 | 15.7% | (many diffs) | |
| 12 | human | 4287 | ATXN3 | ataxin 3 | NR_028455.2 | 14.5% | (many diffs) | |
| 13 | human | 4287 | ATXN3 | ataxin 3 | NR_028453.2 | 14.3% | (many diffs) | |
| 14 | human | 4287 | ATXN3 | ataxin 3 | NR_028461.2 | 14.3% | (many diffs) | |
| 15 | human | 4287 | ATXN3 | ataxin 3 | NR_028457.2 | 14.3% | (many diffs) | |
| 16 | human | 4287 | ATXN3 | ataxin 3 | NR_028462.2 | 14% | (many diffs) | |
| 17 | human | 4287 | ATXN3 | ataxin 3 | NR_028458.2 | 14% | (many diffs) | |
| 18 | human | 4287 | ATXN3 | ataxin 3 | NR_028465.2 | 13.8% | (many diffs) | |
| 19 | human | 4287 | ATXN3 | ataxin 3 | NR_028464.2 | 13.5% | (many diffs) | |
| 20 | human | 4287 | ATXN3 | ataxin 3 | NR_028467.2 | 13.1% | (many diffs) | |
| 21 | human | 4287 | ATXN3 | ataxin 3 | NR_028456.2 | 12.1% | (many diffs) | |
| 22 | human | 4287 | ATXN3 | ataxin 3 | NR_028454.2 | 12% | (many diffs) | |
| 23 | human | 4287 | ATXN3 | ataxin 3 | NR_028469.2 | 11.9% | (many diffs) | |
| 24 | human | 4287 | ATXN3 | ataxin 3 | NR_028463.2 | 9.4% | (many diffs) | |
| 25 | human | 4287 | ATXN3 | ataxin 3 | NR_028466.2 | 9.2% | (many diffs) | |
| 26 | human | 4287 | ATXN3 | ataxin 3 | NM_001164782.2 | 8.7% | 3% | (many diffs) |
| 27 | human | 4287 | ATXN3 | ataxin 3 | NR_031765.2 | 7.3% | (many diffs) | |
| 28 | mouse | 110616 | Atxn3 | ataxin 3 | NM_029705.3 | 84% | 81.9% | (many diffs) |
| 29 | mouse | 110616 | Atxn3 | ataxin 3 | NM_001167914.1 | 70.6% | 73.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1155
- ORF length:
- 1089
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtccatcttc cacgagaaac aagaaggctc actttgtgct caacattgcc 121 tgaataactt attgcaagga gaatatttta gccccgtgga attatcctca attgcacatc 181 agctggatga ggaggagagg atgagaatgg cagaaggagg agttactagt gaagattatc 241 gcacgttttt acagcagcct tctggaaata tggatgacag tggttttttc tctattcagg 301 ttataagcaa tgccttgaaa gtttggggtt tagaactaat cctgttcaac agtccagagt 361 atcagaggct caggatcgat cctataaatg aaagatcatt tatatgcaat tataaggaac 421 actggtttac agttagaaaa ttaggaaaac agtggtttaa cttgaattct ctcttgacgg 481 gtccagaatt aatatcagat acatatcttg cacttttctt ggctcaatta caacaggaag 541 gttattctat atttgtcgtt aagggtgatc tgccagattg cgaagctgac caactcctgc 601 agatgattag ggtccaacag atgcatcgac caaaacttat tggagaagaa ttagcacaac 661 taaaagagca aagagtccat aaaacagacc tggaacgagt gttagaagca aatgatgGCT 721 CAGGAATGTT AGACGAAGAT GAGGAGGATT TGCAGAGGGC TCTGGCACTA AGTCGCCAAG 781 AAATTGACAT GGAAGATGAG GAAGCAGATC TCCGCAGGGC TATTCAGCTA AGTATGCAAG 841 GTAGTTCCAG AAACATATCT CAAGATATGA CACAGACATC AGGTACAAAT CTTACTTCAG 901 AAGAGCTTCG GAAGAGACGA GAAGCCTACT TTGAAAAACA GCAGCAAAAG CAGCAACAGC 961 AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGGG GACCTATCAG 1021 GACAGAGTTC ACATCCATGT GAAAGGCCAG CCACCAGTTC AGGAGCACTT GGGAGTGATC 1081 TAGGTGATGC TATGAGTGAA GAAGACATGC TTCAGGCAGC TGTGACCATG TCTTTAGAAA 1141 CTGTCAGAAA TGATTAGAAA ACAAAAGGAA AAAAATACCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 AAGGGTCAAC TTGCCCGAAT TCAACACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt