Construct: ORF TRCN0000470684

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018901.3_s317c1
Derived from:
ccsbBroadEn_14919
DNA Barcode:
TTAGATAGTCGATTAGTACGTATA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PRPF4B (8899)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470684
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8899 PRPF4B pre-mRNA processing factor 4B NM_003913.5 99.5% 25.9% (many diffs)
2 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011410.2 99.5% 25.9% (many diffs)
3 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011411.2 99.5% 25.9% (many diffs)
4 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011413.2 99.5% 25.9% (many diffs)
5 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011414.2 99.5% 25.9% (many diffs)
6 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011415.2 99.5% 25.9% (many diffs)
7 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011416.2 99.5% 25.9% (many diffs)
8 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011417.2 99.5% 25.9% (many diffs)
9 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011418.2 99.5% 25.9% (many diffs)
10 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011420.2 99.5% 25.9% (many diffs)
11 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011421.2 99.5% 25.9% (many diffs)
12 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011422.2 99.5% 25.9% (many diffs)
13 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011423.2 99.5% 25.9% (many diffs)
14 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011424.2 99.5% 25.9% (many diffs)
15 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011425.2 99.5% 25.9% (many diffs)
16 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011426.2 99.5% 25.9% (many diffs)
17 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011427.2 99.5% 25.9% (many diffs)
18 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011428.2 99.5% 25.9% (many diffs)
19 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011430.2 99.5% 25.9% (many diffs)
20 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011432.2 85.2% 30.1% (many diffs)
21 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011433.2 85.2% 30.1% (many diffs)
22 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011434.2 85.2% 30.1% (many diffs)
23 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011435.2 85.2% 30.1% (many diffs)
24 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146784.2 83.9% (many diffs)
25 human 8899 PRPF4B pre-mRNA processing factor 4B XM_011514970.3 81.4% 31.4% (many diffs)
26 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146783.2 80.9% (many diffs)
27 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146785.2 79.3% (many diffs)
28 mouse 19134 Prpf4b pre-mRNA processing factor 4B NM_013830.2 88.6% 23.1% (many diffs)
29 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315425.1 88.6% 23.1% (many diffs)
30 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315426.1 88.6% 23.1% (many diffs)
31 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315427.1 88.6% 23.1% (many diffs)
32 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315428.1 88.6% 23.1% (many diffs)
33 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315429.1 88.6% 23.1% (many diffs)
34 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315430.1 88.6% 23.1% (many diffs)
35 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315431.1 88.6% 23.1% (many diffs)
36 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315432.1 88.6% 23.1% (many diffs)
37 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315433.1 88.6% 23.1% (many diffs)
38 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315434.1 88.6% 23.1% (many diffs)
39 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315435.1 88.6% 23.1% (many diffs)
40 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315436.1 88.6% 23.1% (many diffs)
41 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315437.1 88.6% 23.1% (many diffs)
42 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_006516582.3 77.2% 26% (many diffs)
43 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_006516583.3 77.1% 26% (many diffs)
44 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_006516584.3 76.8% 26.2% (many diffs)
45 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315438.1 76.8% 26.2% (many diffs)
46 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315439.1 56.1% 33.2% (many diffs)
47 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315440.1 56.1% 33.2% (many diffs)
48 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315441.1 54.7% 37.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
858
ORF length:
789
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggccgccgcg gagacccagt cgctacggga gcagccagag atggaagatg
121ctaattctga aaagagtata aatgaagaaa atggagaagt atcagaagac cagtctcaaa
181ataagcacag tcgtcacaaa aaaaagaagc ataaacacag aagtaaacat aagaaacata
241aacattcctc agaagaagac aaggataaaa aacataaaca taagcataaa cataagaaac
301acaaaagaaa agaggttatt gatgcttctg ataaagaggg tatgtctcca gcaaaaagaa
361ctaaacttga tgatttagct ttgctagaag acttggaaaa acagagagcc ttgattaagg
421ccgaacttga taatgagtta atggaaggaa aggtccagtc tggtatgggg ctcattttgc
481aaggttatga gtctggctct gaagaagagg gggaaattca tgaaaaggca agaaatggaa
541ataggtctag tactagatct tcaagtacaa aggggaaact tgaacttgtg gacaataaaa
601ttactacaaa gaaacgaagt aaaagcagat ccaaagaacg gactagacat aggtctgata
661aaaagaaaag taaggggggt attgaaatcg ttaaagagaa aacaactagg agcaagtcaa
721aggagaggaa aaaatctaaa agcccatcca aaagaagtaa gtctcaagat caagcaagga
781aatcaaaatc ccctaccctt agaaggcgat ctcaagagaa aattggtaag gccagatctc
841ctactgatga taagggttaa aattgaagat aaaagtaaat caaaagatag gaaaaaatcc
901ccaattataa atgaaagtag aagtcgcgat cgaggtaaaa aatccagatc cccagttgat
961ttaagaggta aatccaaaga cagaaggtca cggtccaaag agagaaaatc aaaacggtct
1021gaaactgata aagaaaagaa gccaattaaa tctccctcta aagatgcttc atctgggaaa
1081gaaaataggt cacccagcag aagacctggt cgtagtccta aaagaagaag tttgtctcca
1141aaaccacgtg ataaatcaag aagaagcagg tctccacttt tgaatgatag aagatctaag
1201cagagcaaat ccccctcgcg gacactgtct cctgggagaa gagccaagag ccgatcctta
1261gaaagaaaac gacgagaacc agagaggaga cgactttctt ctccaagaac acgacctcga
1321gatgatatcc tcagtagacg tgaaagatca aaagatgcca gccccatcaa tagatggtct
1381ccaacccgaa gaagaagtag atctcccatt agaaggaggt ctcgttcccc actcagacgt
1441agcaggtctc caagaagaag aagcagatct actcggagaa gggacagagg tcggaggagc
1501agatcacgct tgcgaaggcg gtctcgatca cgcggtggtc gtagacgaag gagcagaagc
1561aaagtaaagg aagataaatt taaaggaagt ctttctgaag gaatgaaagt tgagcaggaa
1621tcttcgtctg atgataacct tgaagacttt gatgtagagg aagaagatga agaagcccta
1681atagaacaga gaagaatcca aaggcaggca attgttcaga aatataaata ccttgctgaa
1741gatagcaaca tgtctgtgcc atctgaacca agcagccccc agagcagtac gagaacacga
1801tcaccatctc cagatgacat tctggagcga gtagctgctg atgttaaaga gtatgaacgg
1861gaaaatgttg atacatttga ggcctcagtg aaagccaagc ataatctaat gacagttgaa
1921cagaataatg gttcatctca gaagaagttg ttggcacctg atatgtttac agaatctgat
1981gatatgtttg ctgcgtattt tgatagtgct cgtcttcggg ccgctggcat tggaaaagat
2041ttcaaagaga atcccaacct cagagataac tggaccgatg cagaaggcta ttatcgtgtg
2101aacataggtg aagtcctaga taaacgttac aatgtgtatg gctacactgg gcaaggtgta
2161ttcagtaatg ttgtacgagc cagagataat gcaagagcca accaagaagt ggctgtaaag
2221atcatcagaa acaatgagct catgcaaaag actggtttaa aagaattaga gttcttgaaa
2281aaacttaatg atgctgatcc tgatgacaaa attcattgtc tgagatcttc aggcactcta
2341tcaccagcag catcttgtct ggtattcgag cctctcagca tgaacttacg agaggtgtaa
2401aaaaatatgt naaagatgtt ggtcttcata ttaagnctgt aagatcctat agtcagcagt
2461tgttcctggc attgaaactc cttaaaagat gcaatatcnt acatgcagat atcaagccag
2521acaatatcct ggttaatgaa tccaaaacta ttttaaagct ttgcgatttt gggtcggctt
2581cacatgttgc ggataatgac ataacacctt atcttgtcag tagattttat cgtgctcctg
2641aaatcattat aggtaaaagc tatgactatg gtatagatat gtggtctgta ggttgcacct
2701tatacgaact ctatactGGA AAAATTTTAT TCCCTGGCAA AACCAATAAC CATATGCTGA
2761AGCTTGCAAT GGATCTCAAA GGAAAGATGC CAAATAAGAT GATTCGAAAA GGTGTGTTCA
2821AAGATCAGCA TTTTGATCAA AATCTCAACT TCATGTACAT AGAAGTTGAT AAAGTAACAG
2881AGAGGGAGAA AGTTACTGTT ATGAGCACCA TTAATCCAAC TAAGGACCTG TTGGCTGACT
2941TGATTGGGTG CCAGAGACTT CCTGAAGACC AACGTAAGAA AGTACACCAG CTAAAGGACT
3001TGTTGGACCA GATTCTGATG TTGGACCCAG CTAAACGAAT TAGCATCAAC CAGGCCCTAC
3061AGCACGCCTT CATCCAGGAA AAAATTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
3121GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
3181AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTAGATAG
3241TCGATTAGTA CGTATAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
3301aagatt

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