Construct: ORF TRCN0000470841
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018789.2_s317c1
- Derived from:
- ccsbBroadEn_15341
- DNA Barcode:
- CGTGCGTGAACAATGGCCCGGCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NME1-NME2 (654364)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470841
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NM_001018136.3 | 99.7% | 8.2% | 36A>N;62_63insG |
| 2 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NR_037149.2 | 62.8% | (many diffs) | |
| 3 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018137.3 | 56.8% | 13.8% | 0_1ins346 |
| 4 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018138.1 | 56.8% | 13.8% | 0_1ins346 |
| 5 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018139.2 | 56.8% | 13.8% | 0_1ins346 |
| 6 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_002512.4 | 56.8% | 13.8% | 0_1ins346 |
| 7 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_000269.2 | 53.6% | 14.4% | (many diffs) |
| 8 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_198175.1 | 49% | 12.4% | (many diffs) |
| 9 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001198682.2 | 30.6% | 25.6% | 0_1ins346;227_228ins113;246_247ins97 |
| 10 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_001077529.2 | 51.3% | 13.8% | (many diffs) |
| 11 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_008705.5 | 51.3% | 13.8% | (many diffs) |
| 12 | mouse | 433968 | Gm5566 | predicted pseudogene 5566 | XR_868740.1 | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 153
- ORF length:
- 84
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaactgt gagcgtacct tcattgcgat caanccagat ggggtccagc 121 ggggtcttgt ggggagagat tatcaagcgt tttgagcaga aaggattccg ccttgttggt 181 ctgaaattca tgcaagcttc cgaagatctt ctcaaggaac actacgttga cctgaaggac 241 cgtccattct ttgccggcct ggtgaaatac atgcactcag ggccggtagt tgccatggtc 301 tgggaggggc tgaatgtggt gaagacgggc cgagtcatgc tcggggagac caaccctgca 361 gactccaagc ctgggaccat ccgtggagac ttctgcatac aagttggcag gaccatggcc 421 aacctggagc gcaccttcat cgccatcaag ccggacggcg tgcagcgcgg cctggtgggc 481 gagatcatca agcgcttcga gcagaaggga ttccgcctcg tggccatgaa gttcctccgg 541 gccTCTGAAG AACACCTGAA GCAGCACTAC ATTGACCTGA AAGACCGACC ATTCTTCCCT 601 GGGCTGGTGA AGTACATGAA CTCAGGGCCG GTTGTGGCCA TGGTCTGGGA GGGGCTGAAC 661 GTGGTGAAGA CAGGCCGAGT GATGCTTGGG GAGACCAATC CAGCAGATTC AAAGCCAGGC 721 ACCATTCGTG GGGACTTCTG CATTCAGGTT GGCAGGAACA TCATTCATGG CAGTGATTCA 781 GTAAAAAGTG CTGAAAAAGA AATCAGCCTA TGGTTTAAGC CTGAAGAACT GGTTGACTAC 841 AAGTCTTGTG CTCATGACTG GGTCTATGAA TTGCCAACTT TCTTGTACAA AGTGGTTGAT 901 ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT 961 CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGTG 1021 CGTGAACAAT GGCCCGGCTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt 1081 gtgaaagatt