Construct: ORF TRCN0000470951

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016413.1_s317c1
Derived from:
ccsbBroadEn_07002
DNA Barcode:
GTCCCTCCTCTCTTCTGCAACACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SSX5 (6758)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470951
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6758 SSX5 SSX family member 5 NM_021015.4 99.7% 99.5% 55G>C;492G>A
2 human 6758 SSX5 SSX family member 5 NM_175723.1 81.8% 81.6% 55G>C;68_69ins123;369G>A
3 human 6758 SSX5 SSX family member 5 XM_011543949.2 81.8% 81.6% 55G>C;68_69ins123;369G>A
4 human 6758 SSX5 SSX family member 5 XM_017029758.2 81.8% 81.6% 55G>C;68_69ins123;369G>A
5 human 10214 SSX3 SSX family member 3 NM_021014.4 76.2% 68.9% (many diffs)
6 human 727837 SSX2B SSX family member 2B NM_001164417.3 76.1% 68.5% (many diffs)
7 human 6757 SSX2 SSX family member 2 NM_175698.2 76.1% 68.5% (many diffs)
8 human 280658 SSX7 SSX family member 7 NM_173358.2 75.6% 67.6% (many diffs)
9 human 6756 SSX1 SSX family member 1 NM_001278691.2 74.2% 62.8% (many diffs)
10 human 6756 SSX1 SSX family member 1 NM_005635.4 74.2% 62.8% (many diffs)
11 human 548313 SSX4B SSX family member 4B NM_001034832.3 73.5% 63.7% (many diffs)
12 human 6759 SSX4 SSX family member 4 NM_005636.4 73.5% 63.7% (many diffs)
13 human 548313 SSX4B SSX family member 4B XM_017029613.1 73.5% 63.7% (many diffs)
14 human 6759 SSX4 SSX family member 4 XM_017029759.1 73.5% 63.7% (many diffs)
15 human 6757 SSX2 SSX family member 2 NM_001278697.1 68.3% 61.5% (many diffs)
16 human 727837 SSX2B SSX family member 2B NM_001278702.2 68.3% 61.5% (many diffs)
17 human 10214 SSX3 SSX family member 3 XM_011543885.2 66.5% 54.1% (many diffs)
18 human 280658 SSX7 SSX family member 7 XM_017029430.1 65.6% 58.7% (many diffs)
19 human 727837 SSX2B SSX family member 2B NM_001278701.2 57.9% 45.6% (many diffs)
20 human 6757 SSX2 SSX family member 2 NM_003147.5 57.9% 45.6% (many diffs)
21 human 548313 SSX4B SSX family member 4B NM_001040612.2 54.1% 41.1% (many diffs)
22 human 6759 SSX4 SSX family member 4 NM_175729.1 54.1% 41.1% (many diffs)
23 human 280658 SSX7 SSX family member 7 XM_017029431.1 52.2% 39.9% (many diffs)
24 human 280657 SSX6P SSX family member 6, pseudo... NR_028366.1 36.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
753
ORF length:
687
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa cggagacgat gcctttgtac ggagacctag ggttggttct caaataccac
121agaagatgca aaagcatccc tggagacaag tctgtgaccg tggaatacat ttggtgaatc
181tcagtccgtt ctggaaggtg ggaagagagc cagccagcag cattaaagct ctactgtgtg
241gcaggggaga agctagggcc ttcgatgata ttgccaaata cttcTCTGAG AAAGAGTGGG
301AAAAGATGAA AGCCTCGGAG AAAATCATCT ATGTGTATAT GAAGAGAAAG TATGAGGCCA
361TGACTAAACT AGGTTTCAAG GCCACCCTCC CACCTTTCAT GCGTAATAAA CGGGTCGCAG
421ACTTCCAGGG GAATGATTTT GATAATGACC CTAACCGTGG GAATCAGGTT GAACATCCTC
481AGATGACTTT CGGCAGGCTC CAGGGAATCT TCCCGAAGAT CACGCCCGAG AAGCCAGCAG
541AGGAAGGAAA TGATTCAAAG GGAGTGCCAG AAGCATCTGG CCCACAGAAC AATGGGAAAC
601AGCTGCGCCC CTCAGGAAAA CTAAATACCT CTGAGAAGGT TAACAAGACA TCTGGACCCA
661AAAGGGGGAA ACATGCCTGG ACCCACAGAG TGCGTGAGAG AAAGCAACTG GTGATTTATG
721AAGAGATCAG CGACCCTCAG GAAGATGACG AGTACCCAAC TTTCTTGTAC AAAGTGGTTG
781ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
841GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT
901CCCTCCTCTC TTCTGCAACA CAACGCGTTA AGTCgacaat caacctctgg attacaaaat
961ttgtgaaaga tt

Download FASTA (ORF) (Full)