Construct: ORF TRCN0000470987

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018849.3_s317c1
Derived from:
ccsbBroadEn_14797
DNA Barcode:
CCGTGGCAATCTGGAAATAATCAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PRKG1 (5592)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470987
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5592 PRKG1 protein kinase cGMP-depende... NM_006258.4 99.8% 63.4% (many diffs)
2 human 5592 PRKG1 protein kinase cGMP-depende... NM_001098512.3 91.6% 52.5% (many diffs)
3 human 5592 PRKG1 protein kinase cGMP-depende... XM_017016412.1 85.7% 48.5% (many diffs)
4 human 5592 PRKG1 protein kinase cGMP-depende... XM_017016413.1 85% 48.2% (many diffs)
5 human 5592 PRKG1 protein kinase cGMP-depende... XM_011539952.2 57.4% 81.8% (many diffs)
6 human 5592 PRKG1 protein kinase cGMP-depende... XM_024448078.1 41.1% 4.8% (many diffs)
7 mouse 19091 Prkg1 protein kinase, cGMP-depend... NM_011160.3 93% 63.1% (many diffs)
8 mouse 19091 Prkg1 protein kinase, cGMP-depend... NM_001013833.3 85.6% 52.3% (many diffs)
9 mouse 19091 Prkg1 protein kinase, cGMP-depend... XM_006526768.3 85.6% 52.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1425
ORF length:
1356
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggcaccttg cgggatttac agtacgcgct ccaggagaag atcgaggagc
121tgaggcagcg ggatgctctc atcgacgagc tggagctgga gttggatcag aaggacgaac
181tgatccagaa gctgcagaac gagctggaca agtaccgctc ggtgatccga ccagccaccc
241agcaggcgca gaagcagagc gcgagcacct tgcagggcga gccgcgcacc aagcggcagg
301cgatctccgc cgagcccacc gccttcgaca tccaggatct cagccatgtg accctgccct
361tctaccccaa gagcccacag tccaaggatc ttataaagga agctatcctt gacaatgact
421ttatgaagaa cttggagctg tcgcagatcc aggagattgt ggattgtatg tacccggtgg
481agtatggcaa ggacagttgc atcatcaaag aaggagacgt ggggtcactg gtgtatgtca
541tggaagatgg taaggttgaa gttacaaaag aaggtgtgaa gttgtgtacc atgggtccag
601gaaaagtgtt tggggaattg gctattcttt acaactgtac ccggacagcg accgtcaaga
661ctcttgtaaa tgtaaaactc tgggccattg atcgacaatg ttttcaaaca ataatgatga
721ggacaggact catcaagcat accgagtata tggaattttt aaaaagcgtt ccaatattcc
781agagccttcc tgaagagatc ctcagcaagc ttgctgatgt ccttgaagag acccactatg
841aaaatggaga atatattatc aggcaaggtg caagagggga caccttcttt atcatcagca
901aaggaacggt aaatgtcact cgtgaagact caccgagtga agacccagtc tttcttagaa
961ctttaggaaa aggagactgg tttggagaga aagccttgca gggggaagat gtgagaacag
1021caaacgtaat tgctgcagaa gctgtaacct gccttgtgat tgacagagac tcttttaaac
1081atttgattgg agggctggat gatgtttcta ataaagcata tgaagatgca gaagctaaag
1141caaaatatga agctgaagcg gctttcttcg ccaacctgaa gctgtctgat ttcaacatca
1201ttgataccct tggagttgga ggtttcggac gagtagaact ggtccagttg aaaagtgaag
1261aatccaaaac gtttgcaatg aagattctca agaaacgtca cattgtggac acaagacagc
1321aggagcacat ccgctcagag aagcagatca tgcagggggc tcattccgat ttcataagtg
1381agactgtaca agaacattta aggacagcaa atatttgtat atgttgatgg aagctgtcta
1441ggtggagagc tctggaccat tctcagggat agaggttcgt ttgaagattc tacaaccaga
1501ttttacacag catgtgtggt agaagctttt gcctatctgc attccaaagg aatcatttac
1561agggacctca agccagaaaa tctcatccta gatcaccgag gttatgccaa actggttgat
1621tttggctttg caaagaaaat aggatttgga aagaaaacat ggactttttg tgggactcca
1681gagtatgtag ccccagagat catcctgaac aaaggccatg acatttcagc cgactactgg
1741tcactgggaa tccTAATGTA TGAACTCCTG ACTGGCAGCC CACCTTTCTC AGGCCCAGAT
1801CCTATGAAAA CCTATAACAT CATATTGAGG GGGATTGACA TGATAGAATT TCCAAAGAAG
1861ATTGCCAAAA ATGCTGCTAA TTTAATTAAA AAACTATGCA GGGACAATCC ATCAGAAAGA
1921TTAGGGAATT TGAAAAATGG AGTAAAAGAC ATTCAAAAGC ACAAATGGTT TGAGGGCTTT
1981AACTGGGAAG GCTTAAGAAA AGGTACCTTG ACACCTCCTA TAATACCAAG TGTTGCATCA
2041CCCACAGACA CAAGTAATTT TGACAGTTTC CCTGAGGACA ACGATGAACC ACCACCTGAT
2101GACAACTCAG GATGGGATAT AGACTTCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC
2161GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG
2221TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT TGTGGAAAGG ACGACCGTGG
2281CAATCTGGAA ATAATCACAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt
2341gaaagatt

Download FASTA (ORF) (Full)