Construct: ORF TRCN0000470987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018849.3_s317c1
- Derived from:
- ccsbBroadEn_14797
- DNA Barcode:
- CCGTGGCAATCTGGAAATAATCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKG1 (5592)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470987
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | NM_006258.4 | 99.8% | 63.4% | (many diffs) |
| 2 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | NM_001098512.3 | 91.6% | 52.5% | (many diffs) |
| 3 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_017016412.1 | 85.7% | 48.5% | (many diffs) |
| 4 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_017016413.1 | 85% | 48.2% | (many diffs) |
| 5 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_011539952.2 | 57.4% | 81.8% | (many diffs) |
| 6 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_024448078.1 | 41.1% | 4.8% | (many diffs) |
| 7 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | NM_011160.3 | 93% | 63.1% | (many diffs) |
| 8 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | NM_001013833.3 | 85.6% | 52.3% | (many diffs) |
| 9 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | XM_006526768.3 | 85.6% | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1425
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaccttg cgggatttac agtacgcgct ccaggagaag atcgaggagc 121 tgaggcagcg ggatgctctc atcgacgagc tggagctgga gttggatcag aaggacgaac 181 tgatccagaa gctgcagaac gagctggaca agtaccgctc ggtgatccga ccagccaccc 241 agcaggcgca gaagcagagc gcgagcacct tgcagggcga gccgcgcacc aagcggcagg 301 cgatctccgc cgagcccacc gccttcgaca tccaggatct cagccatgtg accctgccct 361 tctaccccaa gagcccacag tccaaggatc ttataaagga agctatcctt gacaatgact 421 ttatgaagaa cttggagctg tcgcagatcc aggagattgt ggattgtatg tacccggtgg 481 agtatggcaa ggacagttgc atcatcaaag aaggagacgt ggggtcactg gtgtatgtca 541 tggaagatgg taaggttgaa gttacaaaag aaggtgtgaa gttgtgtacc atgggtccag 601 gaaaagtgtt tggggaattg gctattcttt acaactgtac ccggacagcg accgtcaaga 661 ctcttgtaaa tgtaaaactc tgggccattg atcgacaatg ttttcaaaca ataatgatga 721 ggacaggact catcaagcat accgagtata tggaattttt aaaaagcgtt ccaatattcc 781 agagccttcc tgaagagatc ctcagcaagc ttgctgatgt ccttgaagag acccactatg 841 aaaatggaga atatattatc aggcaaggtg caagagggga caccttcttt atcatcagca 901 aaggaacggt aaatgtcact cgtgaagact caccgagtga agacccagtc tttcttagaa 961 ctttaggaaa aggagactgg tttggagaga aagccttgca gggggaagat gtgagaacag 1021 caaacgtaat tgctgcagaa gctgtaacct gccttgtgat tgacagagac tcttttaaac 1081 atttgattgg agggctggat gatgtttcta ataaagcata tgaagatgca gaagctaaag 1141 caaaatatga agctgaagcg gctttcttcg ccaacctgaa gctgtctgat ttcaacatca 1201 ttgataccct tggagttgga ggtttcggac gagtagaact ggtccagttg aaaagtgaag 1261 aatccaaaac gtttgcaatg aagattctca agaaacgtca cattgtggac acaagacagc 1321 aggagcacat ccgctcagag aagcagatca tgcagggggc tcattccgat ttcataagtg 1381 agactgtaca agaacattta aggacagcaa atatttgtat atgttgatgg aagctgtcta 1441 ggtggagagc tctggaccat tctcagggat agaggttcgt ttgaagattc tacaaccaga 1501 ttttacacag catgtgtggt agaagctttt gcctatctgc attccaaagg aatcatttac 1561 agggacctca agccagaaaa tctcatccta gatcaccgag gttatgccaa actggttgat 1621 tttggctttg caaagaaaat aggatttgga aagaaaacat ggactttttg tgggactcca 1681 gagtatgtag ccccagagat catcctgaac aaaggccatg acatttcagc cgactactgg 1741 tcactgggaa tccTAATGTA TGAACTCCTG ACTGGCAGCC CACCTTTCTC AGGCCCAGAT 1801 CCTATGAAAA CCTATAACAT CATATTGAGG GGGATTGACA TGATAGAATT TCCAAAGAAG 1861 ATTGCCAAAA ATGCTGCTAA TTTAATTAAA AAACTATGCA GGGACAATCC ATCAGAAAGA 1921 TTAGGGAATT TGAAAAATGG AGTAAAAGAC ATTCAAAAGC ACAAATGGTT TGAGGGCTTT 1981 AACTGGGAAG GCTTAAGAAA AGGTACCTTG ACACCTCCTA TAATACCAAG TGTTGCATCA 2041 CCCACAGACA CAAGTAATTT TGACAGTTTC CCTGAGGACA ACGATGAACC ACCACCTGAT 2101 GACAACTCAG GATGGGATAT AGACTTCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC 2161 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 2221 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT TGTGGAAAGG ACGACCGTGG 2281 CAATCTGGAA ATAATCACAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 2341 gaaagatt