Construct: ORF TRCN0000471029
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010154.1_s317c1
- Derived from:
- ccsbBroadEn_10448
- DNA Barcode:
- ATGCGTCAATATGTCCGATAACAC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UGT2B4 (7363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471029
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7363 | UGT2B4 | UDP glucuronosyltransferase... | NM_021139.3 | 99.8% | 99.8% | 1269A>T;1582delG |
| 2 | human | 7364 | UGT2B7 | UDP glucuronosyltransferase... | NM_001074.4 | 90.1% | 85.2% | (many diffs) |
| 3 | human | 10720 | UGT2B11 | UDP glucuronosyltransferase... | NM_001073.3 | 90.1% | 85.6% | (many diffs) |
| 4 | human | 7365 | UGT2B10 | UDP glucuronosyltransferase... | NM_001075.6 | 89.9% | 85.2% | (many diffs) |
| 5 | human | 7365 | UGT2B10 | UDP glucuronosyltransferase... | XM_017008585.2 | 89.4% | 84.6% | (many diffs) |
| 6 | human | 54490 | UGT2B28 | UDP glucuronosyltransferase... | NM_053039.2 | 88.9% | 83.9% | (many diffs) |
| 7 | human | 7367 | UGT2B17 | UDP glucuronosyltransferase... | NM_001077.3 | 85.6% | 77.9% | (many diffs) |
| 8 | human | 7367 | UGT2B17 | UDP glucuronosyltransferase... | XM_024454205.1 | 85.6% | 77.9% | (many diffs) |
| 9 | human | 7366 | UGT2B15 | UDP glucuronosyltransferase... | NM_001076.4 | 84.6% | 78.1% | (many diffs) |
| 10 | human | 10720 | UGT2B11 | UDP glucuronosyltransferase... | XM_011531550.2 | 76.1% | 72.4% | (many diffs) |
| 11 | human | 7365 | UGT2B10 | UDP glucuronosyltransferase... | NM_001144767.3 | 75.9% | 72% | (many diffs) |
| 12 | human | 7363 | UGT2B4 | UDP glucuronosyltransferase... | NM_001297616.1 | 73.4% | 70.8% | (many diffs) |
| 13 | human | 7363 | UGT2B4 | UDP glucuronosyltransferase... | NM_001297615.2 | 69.7% | 68.7% | (many diffs) |
| 14 | human | 54490 | UGT2B28 | UDP glucuronosyltransferase... | XM_017008303.1 | 65.5% | 61.1% | (many diffs) |
| 15 | human | 7364 | UGT2B7 | UDP glucuronosyltransferase... | NM_001330719.1 | 62.4% | 57.5% | (many diffs) |
| 16 | human | 54490 | UGT2B28 | UDP glucuronosyltransferase... | NM_001207004.2 | 55.4% | 50.9% | (many diffs) |
| 17 | human | 10720 | UGT2B11 | UDP glucuronosyltransferase... | XM_017007660.2 | 48.8% | 47.8% | (many diffs) |
| 18 | human | 7364 | UGT2B7 | UDP glucuronosyltransferase... | NM_001349568.1 | 48.7% | 47.4% | (many diffs) |
| 19 | human | 7365 | UGT2B10 | UDP glucuronosyltransferase... | NM_001290091.2 | 48.7% | 47.8% | (many diffs) |
| 20 | human | 101929773 | LOC101929773 | UDP-glucuronosyltransferase... | XM_005275640.3 | 33.7% | 33% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1650
- ORF length:
- 1584
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tatgaaatgg acttcagctc ttctgctgat acagctgagc tgttacttta 121 gctctgggag ttgtggaaag gtgctggtgt ggcccacaga attcagccac tggatgaata 181 taaagacaat cctggatgaa cttgtccaga gaggtcatga ggtgactgta ttggcatctt 241 cagcttccat ttctttcgat cccaacagcc catctactct taaatttgaa gtttatcctg 301 tatctttaac taaaactgag tttgaggata ttatcaagca gctggttaag agatgggcag 361 aacttccaaa agacacattt tggtcatatt tttcacaagt acaagaaatc atgtggacat 421 ttaatgacat acttagaaag ttctgtaagg atatagtttc aaataagaaa cttatgaaga 481 aactacagga gtcaagattt gatgttgttc ttgcagatgc tgttttcccc tttggtgagc 541 tgctggccga gttacttaaa ataccctttg tctacagcct ccgcttctct cctggctacg 601 caattgaaaa gcatagtgga ggacttctgt tccctccttc ctatgtgcct gttgttatgt 661 cagaactaag tgaccaaatg actttcatag agagggtaaa aaatatgatc tatgtgcttt 721 attttgaatt ttggttccaa atatttgaca tgaagaagtg ggatcagttc tacagtgaag 781 ttctaggaag acccactacg ttatctgaga caatggcaaa agctgacata tggcttattc 841 gaaactactg ggattttcaa tttcctcacc cactcttacc aaatgttgag ttcgttggag 901 gactccactg caaacctgcc aaacccctac cgaaggaaat ggaagagttt gtccagagct 961 ctggagaaaa tggtgttgtg gtgttttctc tggggtcgat ggtcagtaac acgtcagaag 1021 aaagggccaa tgtaattgca tcagcccttg ccaagatccc acaaaaggtt ctgtggagat 1081 ttgatgggaa taaaccagat actttaggac tcaatactcg gctgtacaag tggatacccc 1141 agaatgatct tcttggtcac ccaaaaaCCA GAGCTTTTAT AACTCATGGT GGAGCCAATG 1201 GCATCTATGA GGCAATCTAC CATGGAATCC CTATGGTGGG CGTTCCATTG TTTGCAGATC 1261 AACCTGATAA CATTGCACAC ATGAAGGCCA AGGGAGCAGC TGTTAGTTTG GACTTCCACA 1321 CAATGTCGAG TACTGACTTA CTCAATGCAC TGAAGACAGT AATTAATGAT CCTTTATATA 1381 AAGAGAATGC TATGAAATTA TCAAGAATTC ATCATGATCA ACCAGTGAAG CCCCTTGATC 1441 GAGCAGTCTT CTGGATTGAA TTTGTCATGC GCCATAAAGG AGCCAAGCAC CTTCGGGTTG 1501 CAGCCCACGA CCTCACCTGG TTCCAGTACC ACTCTTTGGA TGTGACTGGG TTCCTGCTGG 1561 CCTGTGTGGC AACTGTGATA TTCATCATCA CAAAATGTCT GTTTTGTGTC TGGAAGTTTG 1621 TTAGAACAGG AAAGAAGGGG AAAAGAATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT 1681 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 1741 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATGCGT 1801 CAATATGTCC GATAACACAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 1861 gaaagatt