Construct: ORF TRCN0000471140

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012024.1_s317c1
Derived from:
ccsbBroadEn_13863
DNA Barcode:
AGGCCAAGTCGTGTTGCGCGTACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRID1 (2894)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471140
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2894 GRID1 glutamate ionotropic recept... XM_011539720.2 69.8% 69.7% 5A>T;1828_2616del
2 human 2894 GRID1 glutamate ionotropic recept... NM_017551.3 60.3% 60.2% 5A>T;1828_3027del
3 mouse 14803 Grid1 glutamate receptor, ionotro... XM_011244962.2 61.7% 67.4% (many diffs)
4 mouse 14803 Grid1 glutamate receptor, ionotro... NM_008166.2 54.8% 59.8% (many diffs)
5 mouse 14803 Grid1 glutamate receptor, ionotro... XM_006518565.3 46.1% 50.4% (many diffs)
6 mouse 14803 Grid1 glutamate receptor, ionotro... XM_006518566.3 35.6% 39.1% (many diffs)
7 mouse 14803 Grid1 glutamate receptor, ionotro... XM_006518567.3 29.5% 32.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1893
ORF length:
1827
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt agcgctgacg ctgtggcttc tcccctggat atgccagtgc gtgtcggtgc
121gggccgactc catcatccac atcggtgcca tcttcgagga gaacgcggcc aaggacgaca
181gggtgttcca gttggcggta tccgacctga gcctcaacga tgacatcctg cagagcgaga
241agatcaccta ctccatcaag gtcatcgagg ccaacaaccc attccaggct gtgcaggaag
301cctgtgacct catgacccag gggattttgg ccttggtcac gtccactggc tgtgcatctg
361ccaatgccct gcagtccctc acggatgcca tgcacatccc acacctcttt gtccagcgca
421acccgggagg gtcgccacgc accgcatgcc acctgaaccc cagccccgat ggtgaggcct
481acacactggc ttcgagacca cccgtccgcc tcaatgatgt catgctcagg ctggtgacgg
541agctgcgctg gcagaagttc gtcatgttct acgacagcga gtatgatatc cgtgggcttc
601aaagctttct ggaccaggcc tcgcggctgg gccttgacgt ctctttacaa aaggtggaca
661agaacattag ccacgtattc accagcctct tcaccacgat gaagacagag gagctgaatc
721gctaccggga cacgcttcgc cgcgccatcc tgctgctcag cccacaggga gcccactcct
781tcatcaacga ggccgtggag accaacctgg cttccaagga cagccactgg gtctttgtga
841atgaggaaat cagtgacccg gagatcctgg atctggtcca tagtgccctt ggaaggatga
901ccgtggtccg gcaaatcttt ccgtctgcaa aggacaatca gaaatgcacg aggaacaacc
961accgcatctc ctccctgctc tgcgaccccc aggaaggcta cctccagatg ctgcagatct
1021ccaacctcta tctgtatgac agtgttctga tgctggccaa cgcctttcac aggaagctgg
1081aggaccggaa gtggcatagc atggcgagcc tcaactgcat acggaaatcc actaagccat
1141ggaatggtgg gaggtccatg ctggatacca tcaaaaaggg ccacatcact ggcctcactg
1201gggtgatgga gtttcgggag gacagttcga atccctatgt ccagtttgaa atccttggca
1261ctacctatag tgagactttt ggcaaagaca tgcgcaagtt ggcgacatgg gactcagaga
1321agggcttgaa tggcagcttg caagagaggc ccatgggcag ccgcctccaa ggattgactc
1381ttaaagtggt gactgtcttg gaagagcctt tcgtgatggt ggctgagaac atcctaggac
1441agcccaagcg ctacaaaggg ttctccatag atgtcctgga tgcactggcc aaggctctgg
1501gctttaaata tgagatttac caagcccctg atggcaggta cggtcaccag ctccataaca
1561ccTCCTGGAA CGGGATGATC GGGGAGCTCA TCAGCAAGAG AGCAGACTTG GCCATCTCTG
1621CCATCACCAT CACCCCAGAG AGGGAGAGCG TTGTGGACTT CAGCAAGCGG TACATGGACT
1681ATTCAGTGGG GATTCTAATT AAGAAGCCCG AGGAGAAAAT CAGCATCTTC TCCCTCTTTG
1741CTCCATTTGA TTTCGCTGTG TGGGCCTGCA TTGCAGCAGC CATCCCTGTG GTTGGTGTGC
1801TGATATTTGT GTTGAACAGG ATACAGGCTG TGAGGGCTCA GAGTGCTGCC CAGCCCAGGC
1861CGTCAGCTTC TGCCACTCTG CACAGCGCCA TCTACCCAAC TTTCTTGTAC AAAGTGGTTG
1921ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1981GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG
2041GCCAAGTCGT GTTGCGCGTA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat
2101ttgtgaaaga tt

Download FASTA (ORF) (Full)