Construct: ORF TRCN0000471140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012024.1_s317c1
- Derived from:
- ccsbBroadEn_13863
- DNA Barcode:
- AGGCCAAGTCGTGTTGCGCGTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRID1 (2894)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471140
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2894 | GRID1 | glutamate ionotropic recept... | XM_011539720.2 | 69.8% | 69.7% | 5A>T;1828_2616del |
| 2 | human | 2894 | GRID1 | glutamate ionotropic recept... | NM_017551.3 | 60.3% | 60.2% | 5A>T;1828_3027del |
| 3 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | XM_011244962.2 | 61.7% | 67.4% | (many diffs) |
| 4 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | NM_008166.2 | 54.8% | 59.8% | (many diffs) |
| 5 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | XM_006518565.3 | 46.1% | 50.4% | (many diffs) |
| 6 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | XM_006518566.3 | 35.6% | 39.1% | (many diffs) |
| 7 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | XM_006518567.3 | 29.5% | 32.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1893
- ORF length:
- 1827
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt agcgctgacg ctgtggcttc tcccctggat atgccagtgc gtgtcggtgc 121 gggccgactc catcatccac atcggtgcca tcttcgagga gaacgcggcc aaggacgaca 181 gggtgttcca gttggcggta tccgacctga gcctcaacga tgacatcctg cagagcgaga 241 agatcaccta ctccatcaag gtcatcgagg ccaacaaccc attccaggct gtgcaggaag 301 cctgtgacct catgacccag gggattttgg ccttggtcac gtccactggc tgtgcatctg 361 ccaatgccct gcagtccctc acggatgcca tgcacatccc acacctcttt gtccagcgca 421 acccgggagg gtcgccacgc accgcatgcc acctgaaccc cagccccgat ggtgaggcct 481 acacactggc ttcgagacca cccgtccgcc tcaatgatgt catgctcagg ctggtgacgg 541 agctgcgctg gcagaagttc gtcatgttct acgacagcga gtatgatatc cgtgggcttc 601 aaagctttct ggaccaggcc tcgcggctgg gccttgacgt ctctttacaa aaggtggaca 661 agaacattag ccacgtattc accagcctct tcaccacgat gaagacagag gagctgaatc 721 gctaccggga cacgcttcgc cgcgccatcc tgctgctcag cccacaggga gcccactcct 781 tcatcaacga ggccgtggag accaacctgg cttccaagga cagccactgg gtctttgtga 841 atgaggaaat cagtgacccg gagatcctgg atctggtcca tagtgccctt ggaaggatga 901 ccgtggtccg gcaaatcttt ccgtctgcaa aggacaatca gaaatgcacg aggaacaacc 961 accgcatctc ctccctgctc tgcgaccccc aggaaggcta cctccagatg ctgcagatct 1021 ccaacctcta tctgtatgac agtgttctga tgctggccaa cgcctttcac aggaagctgg 1081 aggaccggaa gtggcatagc atggcgagcc tcaactgcat acggaaatcc actaagccat 1141 ggaatggtgg gaggtccatg ctggatacca tcaaaaaggg ccacatcact ggcctcactg 1201 gggtgatgga gtttcgggag gacagttcga atccctatgt ccagtttgaa atccttggca 1261 ctacctatag tgagactttt ggcaaagaca tgcgcaagtt ggcgacatgg gactcagaga 1321 agggcttgaa tggcagcttg caagagaggc ccatgggcag ccgcctccaa ggattgactc 1381 ttaaagtggt gactgtcttg gaagagcctt tcgtgatggt ggctgagaac atcctaggac 1441 agcccaagcg ctacaaaggg ttctccatag atgtcctgga tgcactggcc aaggctctgg 1501 gctttaaata tgagatttac caagcccctg atggcaggta cggtcaccag ctccataaca 1561 ccTCCTGGAA CGGGATGATC GGGGAGCTCA TCAGCAAGAG AGCAGACTTG GCCATCTCTG 1621 CCATCACCAT CACCCCAGAG AGGGAGAGCG TTGTGGACTT CAGCAAGCGG TACATGGACT 1681 ATTCAGTGGG GATTCTAATT AAGAAGCCCG AGGAGAAAAT CAGCATCTTC TCCCTCTTTG 1741 CTCCATTTGA TTTCGCTGTG TGGGCCTGCA TTGCAGCAGC CATCCCTGTG GTTGGTGTGC 1801 TGATATTTGT GTTGAACAGG ATACAGGCTG TGAGGGCTCA GAGTGCTGCC CAGCCCAGGC 1861 CGTCAGCTTC TGCCACTCTG CACAGCGCCA TCTACCCAAC TTTCTTGTAC AAAGTGGTTG 1921 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1981 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 2041 GCCAAGTCGT GTTGCGCGTA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2101 ttgtgaaaga tt