Construct: ORF TRCN0000471271
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004437.1_s317c1
- Derived from:
- ccsbBroadEn_03309
- DNA Barcode:
- AACGGCGGTGCTAGGCGGGGCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF138 (51444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471271
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51444 | RNF138 | ring finger protein 138 | NM_001191324.1 | 100% | 100% | |
| 2 | human | 51444 | RNF138 | ring finger protein 138 | NM_016271.5 | 100% | 100% | |
| 3 | human | 51444 | RNF138 | ring finger protein 138 | XM_005258285.1 | 81.2% | 81.2% | 0_1ins138 |
| 4 | human | 51444 | RNF138 | ring finger protein 138 | NM_198128.2 | 61.6% | 61.6% | 110_111ins282 |
| 5 | human | 51444 | RNF138 | ring finger protein 138 | XM_005258286.2 | 61.6% | 61.6% | 110_111ins282 |
| 6 | human | 51444 | RNF138 | ring finger protein 138 | XR_935225.2 | 20.8% | 1_455del;1191_3519del | |
| 7 | human | 51444 | RNF138 | ring finger protein 138 | XR_001753203.2 | 12.8% | 1_455del;565_566ins282;909_3237del | |
| 8 | human | 379013 | RNF138P1 | ring finger protein 138 pse... | NR_001575.1 | 12.1% | (many diffs) | |
| 9 | mouse | 56515 | Rnf138 | ring finger protein 138 | NM_207623.2 | 93.3% | 93.8% | (many diffs) |
| 10 | mouse | 56515 | Rnf138 | ring finger protein 138 | XM_011246975.2 | 93.3% | 93.8% | (many diffs) |
| 11 | mouse | 56515 | Rnf138 | ring finger protein 138 | XM_006526117.3 | 86.2% | 86.9% | (many diffs) |
| 12 | mouse | 56515 | Rnf138 | ring finger protein 138 | NM_019706.3 | 79% | 79.1% | (many diffs) |
| 13 | mouse | 56515 | Rnf138 | ring finger protein 138 | NM_001303011.1 | 71.9% | 72.2% | (many diffs) |
| 14 | mouse | 56515 | Rnf138 | ring finger protein 138 | XM_006526118.3 | 71.9% | 72.2% | (many diffs) |
| 15 | mouse | 56515 | Rnf138 | ring finger protein 138 | XM_006526119.3 | 50.2% | 52.2% | (many diffs) |
| 16 | mouse | 56515 | Rnf138 | ring finger protein 138 | NM_001303005.1 | 44.8% | 44% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 801
- ORF length:
- 735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgaggacctc tctgcggcca cgtcctacac cgaagatgat ttctactgcc 121 ccgtctgtca ggaggtgctc aaaacgcccg tgcggaccac ggcctgtcag cacgttttct 181 gtagaaaatg tttcctgact gcaatgaggg aaagcggagc acattgtccc ctatgtcgtg 241 gaaatgtgac tagaagagag agagcatgtc ctgaacgggc cttagacctt gaaaatataa 301 tgaggaagtt ttctggtagc tgcagatgct gtgcaaaaca gattaaattc tatcgcatga 361 gacatcatta caaatcttgt aagaagtatc aggatgaata tggtgttTCT TCTATCATTC 421 CAAACTTTCA GATCTCTCAA GATTCAGTAG GGAACAGCAA TAGGAGTGAA ACATCCACAT 481 CTGATAACAC AGAAACTTAC CAAGAGAATA CAAGTTCTTC TGGTCATCCT ACTTTTAAGT 541 GTCCCCTGTG TCAAGAATCA AATTTTACCA GACAGCGTTT ACTGGATCAC TGTAACAGTA 601 ATCACCTATT TCAGATAGTT CCTGTGACAT GTCCTATTTG TGTGTCTCTT CCTTGGGGAG 661 ATCCTAGCCA GATTACCAGA AATTTCGTTA GTCATCTAAA TCAGAGACAT CAATTTGATT 721 ATGGAGAATT TGTGAATCTT CAGCTAGATG AAGAAACCCA ATACCAAACT GCTGTTGAAG 781 AATCTTTTCA AGTAAACATC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 841 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 901 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACG GCGGTGCTAG 961 GCGGGGCCCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt