Construct: ORF TRCN0000471919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003419.3_s317c1
- Derived from:
- ccsbBroadEn_15120
- DNA Barcode:
- TTCACAAAGACGCGCTTACACAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK5 (57144)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471919
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | NM_020341.4 | 99.8% | 99.7% | 79C>G;1208C>A;2127C>T |
| 2 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | NM_177990.3 | 99.8% | 99.7% | 79C>G;1208C>A;2127C>T |
| 3 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027960.1 | 99.8% | 99.7% | 79C>G;1208C>A;2127C>T |
| 4 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027961.1 | 99.8% | 99.7% | 79C>G;1208C>A;2127C>T |
| 5 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027962.1 | 99.8% | 99.7% | 79C>G;1208C>A;2127C>T |
| 6 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027963.1 | 99.8% | 99.7% | 79C>G;1208C>A;2127C>T |
| 7 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027964.1 | 99.8% | 99.7% | 79C>G;1208C>A;2127C>T |
| 8 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027965.1 | 99.8% | 99.7% | 79C>G;1208C>A;2127C>T |
| 9 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | NM_172858.2 | 89.6% | 93.6% | (many diffs) |
| 10 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_006499509.1 | 89.6% | 93.6% | (many diffs) |
| 11 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_011239531.1 | 89.6% | 93.6% | (many diffs) |
| 12 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_017318217.1 | 89.6% | 93.6% | (many diffs) |
| 13 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_017318218.1 | 89.6% | 93.6% | (many diffs) |
| 14 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_006499508.3 | 87.5% | 91.4% | (many diffs) |
| 15 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_017318219.1 | 57.7% | 61.7% | (many diffs) |
| 16 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_006499510.3 | 56.3% | 60.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2223
- ORF length:
- 2157
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgggaagaaa aagaaaaaga ttgaaatatc tggcccgtcc aactttgaac 121 acagggttca tactgggttt gatgcacaag agcagaagtt taccggcctt ccccagcagt 181 ggcacagcct gttagcagat acggccaaca ggccaaagcc tatggtggac ccttcatgca 241 tcacacccat ccagctggct cctatgaaga caatcgttag aggaaacaaa ccctgcaagg 301 aaacctccat caacggcctg ctagaggatt ttgacaacat ctcggtgact cgctccaact 361 ccctaaggaa agaaagccca cccaccccag atcagggagc ctccagccac ggtccaggcc 421 acgcggaaga aaatggcttc atcaccttct cccagtattc cagcgaatcc gatactactg 481 ctgactacac gaccgaaaag tacagggaga agagtctcta tggagatgat ctggatccgt 541 attatagagg cagccacgca gccaagcaaa atgggcacgt aatgaaaatg aagcacgggg 601 aggcctacta ttctgaggtg aagcctttga aatccgattt tgccagattt tctgccgatt 661 atcactcaca tttggactca ctgagcaaac caagtgaata cagtgacctc aagtgggagt 721 atcagagagc ctcgagtagc tcccctctgg attattcatt ccaattcaca ccttctagaa 781 ctgcagggac cagcgggtgc tccaaggaga gcctggcgta cagtgaaagt gaatggggac 841 ccagcctgga tgactatgac aggaggccaa agtcttcgta cctgaatcag acaagccctc 901 agcccaccat gcggcagagg tccaggtcag gctcgggact ccaggaaccg atgatgccat 961 ttggagcaag tgcatttaaa acccatcccc aaggacactc ctacaactcc tacacctacc 1021 ctcgcttgtc cgagcccaca atgtgcattc caaaggtgga ttacgatcga gcacagatgg 1081 tcctcagccc tccactgtca gggtctgaca cctaccccag gggccctgcc aaactacctc 1141 aaagtcaaag caaatcgggc tattcctcaa gcagtcacca gtacccgtct gggtaccaca 1201 aagccacctt gtaccatcac ccctccctgc agagcagttc gcagtacatc tccacggctt 1261 cctacctgag ctacctcagc ctctcatcca gcacctaccc gccgcccagc tggggctcct 1321 cctccgacca gcagccctcc agggtgtccc atgaacagtt tcgggcggcc ctgcagctgg 1381 tggtcagccc aggagacccc agggaatact tggccaactt tatcaaaatc ggggaaggct 1441 caaccggcat cgtatgcatc gccaccgaga aacacacagg gaaacaagtt gcagtgaaga 1501 aaatggacct ccggaagcaa cagagacgag aactgctttt caatgaggtc gtgatcatgc 1561 gggattacca ccatgacaat gtggttgaca tgtacagcag ctaccttgtc ggcgatgagc 1621 tctgggtggt catggagttt ctagaaggtg gtgccttgac agacattgtg actcacacca 1681 gaatgaatga agaacagata gctactgtct gcctgtcagt tctgagagct ctctcctacc 1741 ttcataacca aggagtgatt cacagggaca taaaaagtga ctccatcctc ctgacaagcg 1801 atggccggat aaagttgtct gattttggtt TCTGTGCTCA AGTTTCCAAA GAGGTGCCGA 1861 AGAGGAAATC ATTGGTTGGC ACTCCCTACT GGATGGCCCC TGAGGTGATT TCTAGGCTAC 1921 CTTATGGGAC AGAGGTGGAC ATCTGGTCCC TCGGGATCAT GGTGATAGAA ATGATTGATG 1981 GCGAGCCCCC CTACTTCAAT GAGCCTCCCC TCCAGGCGAT GCGGAGGATC CGGGACAGTT 2041 TACCTCCAAG AGTGAAGGAC CTACACAAGG TTTCTTCAGT GCTCCGGGGA TTCCTAGACT 2101 TGATGTTGGT GAGGGAGCCC TCTCAGAGAG CAACAGCCCA GGAACTCCTC GGACATCCAT 2161 TCTTAAAACT AGCAGGTCCA CCGTCTTGCA TTGTCCCCCT CATGAGACAA TACAGGCATC 2221 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2281 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2341 GGCTTTATAT ATCTTGTGGA AAGGACGATT CACAAAGACG CGCTTACACA GAACGCGTTA 2401 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt