Construct: ORF TRCN0000473048

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003913.1_s317c1
Derived from:
ccsbBroadEn_03547
DNA Barcode:
AGTGCATTTCATCTCTGTAGCAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FAM86C1 (55199)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473048
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55199 FAM86C1 family with sequence simila... NM_152563.2 100% 100%
2 human 55199 FAM86C1 family with sequence simila... NM_001099653.1 82.9% 82.9% 157_237del
3 human 55199 FAM86C1 family with sequence simila... NM_018172.3 79.3% 79.3% 159_260del
4 human 85002 FAM86B1 family with sequence simila... XM_017013919.2 57.9% 56.3% (many diffs)
5 human 85002 FAM86B1 family with sequence simila... XM_006716260.4 45.4% 21% (many diffs)
6 human 196483 EEF2KMT eukaryotic elongation facto... NM_001289029.1 45.1% 22.6% (many diffs)
7 human 85002 FAM86B1 family with sequence simila... NM_001083537.2 41.3% 21.4% (many diffs)
8 human 196483 EEF2KMT eukaryotic elongation facto... NM_201598.3 41.1% 22.6% (many diffs)
9 human 196483 EEF2KMT eukaryotic elongation facto... XM_005255157.4 40.1% 20.5% (many diffs)
10 human 85002 FAM86B1 family with sequence simila... XM_006716256.4 37% 17.2% (many diffs)
11 human 653333 FAM86B2 family with sequence simila... NM_001137610.2 36.9% 19.1% (many diffs)
12 human 196483 EEF2KMT eukaryotic elongation facto... NM_201400.4 36.9% 19.1% (many diffs)
13 human 85002 FAM86B1 family with sequence simila... XM_017013917.2 34.8% 19.2% (many diffs)
14 human 653333 FAM86B2 family with sequence simila... NR_148877.1 33.4% (many diffs)
15 human 85002 FAM86B1 family with sequence simila... XM_017013918.2 32.1% 17% (many diffs)
16 human 85002 FAM86B1 family with sequence simila... XM_017013916.2 32% 19.6% (many diffs)
17 human 85002 FAM86B1 family with sequence simila... XM_006716257.4 31.4% 18.3% (many diffs)
18 human 653333 FAM86B2 family with sequence simila... NR_148876.1 31.1% (many diffs)
19 human 653333 FAM86B2 family with sequence simila... NR_148878.1 24.6% (many diffs)
20 human 645332 FAM86C2P family with sequence simila... NR_024249.1 19.9% (many diffs)
21 human 100125556 FAM86JP family with sequence simila... NR_024250.1 18% (many diffs)
22 human 100125556 FAM86JP family with sequence simila... NR_024251.1 17.2% (many diffs)
23 human 85002 FAM86B1 family with sequence simila... NR_003494.2 16.8% (many diffs)
24 human 85002 FAM86B1 family with sequence simila... XR_001745614.2 16.6% (many diffs)
25 human 653113 FAM86FP family with sequence simila... NR_024254.1 16.4% (many diffs)
26 human 85002 FAM86B1 family with sequence simila... XR_001745612.2 15.8% (many diffs)
27 human 729375 FAM86HP family with sequence simila... NR_024252.1 15% (many diffs)
28 human 85002 FAM86B1 family with sequence simila... XR_001745613.2 15% (many diffs)
29 human 348926 FAM86EP family with sequence simila... NR_130742.1 14.6% (many diffs)
30 human 692099 FAM86DP family with sequence simila... NR_024241.1 14.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
459
ORF length:
393
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gcccgaggag aacgcggGGA GCGAACTCTT GCTGCAGAGT TTCAAGCGCC
121GCTTCCTGGC AGCGCGCGCC CTGCGCTCCT TCCGCTGGCA GAGCTTAGAA GCAAAGTTAA
181GAGACTCATC AGATTCTGAG CTGCTGCGGG ATATTTTGCA GAAGACGTGC TGTATTGCCC
241AGAAGCCATC GTGTCGCTGG TCGGGGTCCT GCGGAGGCTG GCTGCCTGCC GGGAGCACCA
301GCGGGCTCCT CAATTCTACA TGGCCCTTAC CGTCTGCAAC CCAGAGATGT GCCAGCTGTT
361CACCACCGAG CTATGCTGGA CTGGGATCAG ATGGGAAGCG GAAGCTCATC ATGACCAGAA
421ACTGTTTCCC TACAGAGAGC ACTTGGAGAT GGCAAAGCTG CCCAACTTTC TTGTACAAAG
481TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
541GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
601ACGAAGTGCA TTTCATCTCT GTAGCAACAC GCGTTAAGTC gacaatcaac ctctggatta
661caaaatttgt gaaagatt

Download FASTA (ORF) (Full)