Construct: ORF TRCN0000473048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003913.1_s317c1
- Derived from:
- ccsbBroadEn_03547
- DNA Barcode:
- AGTGCATTTCATCTCTGTAGCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM86C1 (55199)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473048
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55199 | FAM86C1 | family with sequence simila... | NM_152563.2 | 100% | 100% | |
| 2 | human | 55199 | FAM86C1 | family with sequence simila... | NM_001099653.1 | 82.9% | 82.9% | 157_237del |
| 3 | human | 55199 | FAM86C1 | family with sequence simila... | NM_018172.3 | 79.3% | 79.3% | 159_260del |
| 4 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013919.2 | 57.9% | 56.3% | (many diffs) |
| 5 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716260.4 | 45.4% | 21% | (many diffs) |
| 6 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_001289029.1 | 45.1% | 22.6% | (many diffs) |
| 7 | human | 85002 | FAM86B1 | family with sequence simila... | NM_001083537.2 | 41.3% | 21.4% | (many diffs) |
| 8 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_201598.3 | 41.1% | 22.6% | (many diffs) |
| 9 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | XM_005255157.4 | 40.1% | 20.5% | (many diffs) |
| 10 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716256.4 | 37% | 17.2% | (many diffs) |
| 11 | human | 653333 | FAM86B2 | family with sequence simila... | NM_001137610.2 | 36.9% | 19.1% | (many diffs) |
| 12 | human | 196483 | EEF2KMT | eukaryotic elongation facto... | NM_201400.4 | 36.9% | 19.1% | (many diffs) |
| 13 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013917.2 | 34.8% | 19.2% | (many diffs) |
| 14 | human | 653333 | FAM86B2 | family with sequence simila... | NR_148877.1 | 33.4% | (many diffs) | |
| 15 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013918.2 | 32.1% | 17% | (many diffs) |
| 16 | human | 85002 | FAM86B1 | family with sequence simila... | XM_017013916.2 | 32% | 19.6% | (many diffs) |
| 17 | human | 85002 | FAM86B1 | family with sequence simila... | XM_006716257.4 | 31.4% | 18.3% | (many diffs) |
| 18 | human | 653333 | FAM86B2 | family with sequence simila... | NR_148876.1 | 31.1% | (many diffs) | |
| 19 | human | 653333 | FAM86B2 | family with sequence simila... | NR_148878.1 | 24.6% | (many diffs) | |
| 20 | human | 645332 | FAM86C2P | family with sequence simila... | NR_024249.1 | 19.9% | (many diffs) | |
| 21 | human | 100125556 | FAM86JP | family with sequence simila... | NR_024250.1 | 18% | (many diffs) | |
| 22 | human | 100125556 | FAM86JP | family with sequence simila... | NR_024251.1 | 17.2% | (many diffs) | |
| 23 | human | 85002 | FAM86B1 | family with sequence simila... | NR_003494.2 | 16.8% | (many diffs) | |
| 24 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745614.2 | 16.6% | (many diffs) | |
| 25 | human | 653113 | FAM86FP | family with sequence simila... | NR_024254.1 | 16.4% | (many diffs) | |
| 26 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745612.2 | 15.8% | (many diffs) | |
| 27 | human | 729375 | FAM86HP | family with sequence simila... | NR_024252.1 | 15% | (many diffs) | |
| 28 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745613.2 | 15% | (many diffs) | |
| 29 | human | 348926 | FAM86EP | family with sequence simila... | NR_130742.1 | 14.6% | (many diffs) | |
| 30 | human | 692099 | FAM86DP | family with sequence simila... | NR_024241.1 | 14.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcccgaggag aacgcggGGA GCGAACTCTT GCTGCAGAGT TTCAAGCGCC 121 GCTTCCTGGC AGCGCGCGCC CTGCGCTCCT TCCGCTGGCA GAGCTTAGAA GCAAAGTTAA 181 GAGACTCATC AGATTCTGAG CTGCTGCGGG ATATTTTGCA GAAGACGTGC TGTATTGCCC 241 AGAAGCCATC GTGTCGCTGG TCGGGGTCCT GCGGAGGCTG GCTGCCTGCC GGGAGCACCA 301 GCGGGCTCCT CAATTCTACA TGGCCCTTAC CGTCTGCAAC CCAGAGATGT GCCAGCTGTT 361 CACCACCGAG CTATGCTGGA CTGGGATCAG ATGGGAAGCG GAAGCTCATC ATGACCAGAA 421 ACTGTTTCCC TACAGAGAGC ACTTGGAGAT GGCAAAGCTG CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGAAGTGCA TTTCATCTCT GTAGCAACAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt