Transcript: Human NM_001099653.1

Homo sapiens family with sequence similarity 86 member C1 (FAM86C1), transcript variant 3, mRNA.

Source:
NCBI, updated 2018-06-24
Taxon:
Homo sapiens (human)
Gene:
FAM86C1 (55199)
Length:
2106
CDS:
27..503

Additional Resources:

NCBI RefSeq record:
NM_001099653.1
NBCI Gene record:
FAM86C1 (55199)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001099653.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000426475 TATGCTGGACTGGGATCAGAT pLKO_005 414 CDS 100% 4.950 3.465 N FAM86C1 n/a
2 TRCN0000422045 CACACTCGTTTCCTCACAAAG pLKO_005 883 3UTR 100% 10.800 6.480 N FAM86C1 n/a
3 TRCN0000444970 CTGAGCTGCTGCGGGATATTT pLKO_005 157 CDS 100% 15.000 7.500 Y EEF2KMT n/a
4 TRCN0000430453 AGAGCTTAGAAGCAAAGTTAA pLKO_005 121 CDS 100% 13.200 6.600 Y EEF2KMT n/a
5 TRCN0000168044 CCAACGGGATTGTGAGAATTA pLKO.1 533 3UTR 100% 13.200 6.600 Y FAM86C1 n/a
6 TRCN0000172901 GCCAGCAGTTCTGGTTCTTAA pLKO.1 706 3UTR 100% 13.200 6.600 Y EEF2KMT n/a
7 TRCN0000447299 TCTGAGCTGCTGCGGGATATT pLKO_005 156 CDS 100% 13.200 6.600 Y FAM86C1 n/a
8 TRCN0000427069 TTTATATGGGAAAGCGGATAA pLKO_005 576 3UTR 100% 10.800 5.400 Y FAM86C1 n/a
9 TRCN0000168498 GCAGAGCTTAGAAGCAAAGTT pLKO.1 119 CDS 100% 5.625 2.813 Y FAM86C1 n/a
10 TRCN0000128827 GCTTTCTCTCAGAACTCATCA pLKO.1 241 CDS 100% 4.950 2.475 Y FAM86B1 n/a
11 TRCN0000129161 GTGCTTTCTCTCAGAACTCAT pLKO.1 239 CDS 100% 4.950 2.475 Y FAM86B1 n/a
12 TRCN0000172453 CGAACTCTTGCTGCAGAGTTT pLKO.1 53 CDS 100% 0.495 0.248 Y FAM86C1 n/a
13 TRCN0000130496 GACCAGAAACTGTTTCCCTAT pLKO.1 455 CDS 100% 4.050 2.025 Y FAM86B1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001099653.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03547 pDONR223 100% 82.9% 82.9% None 157_237del n/a
2 ccsbBroad304_03547 pLX_304 0% 82.9% 82.9% V5 157_237del n/a
3 TRCN0000473048 AGTGCATTTCATCTCTGTAGCAAC pLX_317 92.4% 82.9% 82.9% V5 157_237del n/a
4 TRCN0000488399 TACCTGCGCGCCTAACAATCATGA pLX_317 37.3% 44.8% 26.4% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_09791 pDONR223 100% 44.6% 26.8% None (many diffs) n/a
6 ccsbBroad304_09791 pLX_304 0% 44.6% 26.8% V5 (many diffs) n/a
7 TRCN0000466174 GGAGAGATGACCTTCCATCATCTG pLX_317 21.3% 44.6% 26.8% V5 (many diffs) n/a
8 TRCN0000488297 ACTAGACGAAAAATGAGGCATGGA pLX_317 30.4% 44.6% 26.8% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000491961 CCGTTCATGATGGCGTAAGTACGC pLX_317 33.3% 38.1% 23% V5 (not translated due to prior stop codon) (many diffs) n/a
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