Transcript: Human XM_006716256.4

PREDICTED: Homo sapiens family with sequence similarity 86 member B1 (FAM86B1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM86B1 (85002)
Length:
2578
CDS:
90..1082

Additional Resources:

NCBI RefSeq record:
XM_006716256.4
NBCI Gene record:
FAM86B1 (85002)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006716256.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000444970 CTGAGCTGCTGCGGGATATTT pLKO_005 220 CDS 100% 15.000 7.500 Y EEF2KMT n/a
2 TRCN0000447299 TCTGAGCTGCTGCGGGATATT pLKO_005 219 CDS 100% 13.200 6.600 Y FAM86C1 n/a
3 TRCN0000172312 CGAGGGAATGTCCTTCTCAAT pLKO.1 681 CDS 100% 4.950 2.475 Y EEF2KMT n/a
4 TRCN0000128827 GCTTTCTCTCAGAACTCATCA pLKO.1 304 CDS 100% 4.950 2.475 Y FAM86B1 n/a
5 TRCN0000129161 GTGCTTTCTCTCAGAACTCAT pLKO.1 302 CDS 100% 4.950 2.475 Y FAM86B1 n/a
6 TRCN0000130496 GACCAGAAACTGTTTCCCTAT pLKO.1 1020 CDS 100% 4.050 2.025 Y FAM86B1 n/a
7 TRCN0000168609 GATGTTGTCATTGCAGCAGAT pLKO.1 813 CDS 100% 4.050 2.025 Y EEF2KMT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006716256.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488399 TACCTGCGCGCCTAACAATCATGA pLX_317 37.3% 94.6% 91.8% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_09791 pDONR223 100% 94.5% 91.8% None (many diffs) n/a
3 ccsbBroad304_09791 pLX_304 0% 94.5% 91.8% V5 (many diffs) n/a
4 TRCN0000466174 GGAGAGATGACCTTCCATCATCTG pLX_317 21.3% 94.5% 91.8% V5 (many diffs) n/a
5 TRCN0000488297 ACTAGACGAAAAATGAGGCATGGA pLX_317 30.4% 94.5% 91.8% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000491961 CCGTTCATGATGGCGTAAGTACGC pLX_317 33.3% 80.7% 78.2% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_03547 pDONR223 100% 37% 17.2% None (many diffs) n/a
8 ccsbBroad304_03547 pLX_304 0% 37% 17.2% V5 (many diffs) n/a
9 TRCN0000473048 AGTGCATTTCATCTCTGTAGCAAC pLX_317 92.4% 37% 17.2% V5 (many diffs) n/a
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