Construct: ORF TRCN0000473555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013385.1_s317c1
- Derived from:
- ccsbBroadEn_00915
- DNA Barcode:
- AATCGCAAATACTACCTAGATCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRTAP5-9 (3846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3846 | KRTAP5-9 | keratin associated protein 5-9 | NM_005553.3 | 100% | 100% | |
| 2 | human | 440051 | KRTAP5-11 | keratin associated protein ... | NM_001005405.3 | 76.2% | 72.4% | (many diffs) |
| 3 | human | 57830 | KRTAP5-8 | keratin associated protein 5-8 | NM_021046.3 | 75.9% | 74.2% | (many diffs) |
| 4 | human | 440050 | KRTAP5-7 | keratin associated protein 5-7 | NM_001012503.2 | 75% | 72.8% | (many diffs) |
| 5 | human | 387273 | KRTAP5-10 | keratin associated protein ... | NM_001012710.2 | 68.3% | 62.9% | (many diffs) |
| 6 | human | 440021 | KRTAP5-2 | keratin associated protein 5-2 | NM_001004325.2 | 67% | 58.5% | (many diffs) |
| 7 | human | 387266 | KRTAP5-3 | keratin associated protein 5-3 | NM_001012708.2 | 65.1% | 63% | (many diffs) |
| 8 | human | 440023 | KRTAP5-6 | keratin associated protein 5-6 | NM_001012416.1 | 64.1% | 60.5% | (many diffs) |
| 9 | human | 387267 | KRTAP5-4 | keratin associated protein 5-4 | NM_001347674.1 | 61.6% | 61.8% | (many diffs) |
| 10 | human | 439915 | KRTAP5-5 | keratin associated protein 5-5 | NM_001001480.2 | 58.8% | 55.6% | (many diffs) |
| 11 | human | 439915 | KRTAP5-5 | keratin associated protein 5-5 | XM_006725534.2 | 57% | 54.5% | (many diffs) |
| 12 | human | 387264 | KRTAP5-1 | keratin associated protein 5-1 | NM_001005922.1 | 53.8% | 48.5% | (many diffs) |
| 13 | human | 387267 | KRTAP5-4 | keratin associated protein 5-4 | NM_001012709.1 | 49.3% | 49.3% | (many diffs) |
| 14 | mouse | 114666 | Krtap5-5 | keratin associated protein 5-5 | NM_001037822.1 | 60.5% | 57.2% | (many diffs) |
| 15 | mouse | 105243090 | LOC105243090 | keratin-associated protein ... | NM_001347555.1 | 60.2% | 58.3% | (many diffs) |
| 16 | mouse | 105243089 | Gm39115 | predicted gene, 39115 | NM_001347556.1 | 58.7% | 53.1% | (many diffs) |
| 17 | mouse | 50774 | Krtap5-1 | keratin associated protein 5-1 | NM_015808.1 | 56.6% | 59.1% | (many diffs) |
| 18 | mouse | 105244938 | Gm40460 | predicted gene, 40460 | XM_017312478.1 | 55.8% | 52.9% | (many diffs) |
| 19 | mouse | 50775 | Krtap5-4 | keratin associated protein 5-4 | NM_015809.2 | 53.8% | 53.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgctgtggc tgcTCCGGAG GCTGTGGCTC CAGCTGTGGA GGCTGTGACT 121 CCAGCTGTGG GAGCTGTGGC TCTGGCTGCA GGGGCTGTGG CCCCAGCTGC TGTGCACCCG 181 TCTACTGCTG CAAGCCCGTG TGCTGCTGTG TTCCAGCCTG TTCCTGCTCT AGCTGTGGCA 241 AGCGGGGCTG TGGCTCCTGT GGGGGCTCCA AGGGAGGCTG TGGTTCTTGT GGCTGCTCCC 301 AGTGCAGTTG CTGCAAGCCC TGCTGTTGCT CTTCAGGCTG TGGGTCATCC TGCTGCCAGT 361 GCAGCTGCTG CAAGCCCTAC TGCTCCCAGT GCAGCTGCTG TAAGCCCTGT TGCTCCTCCT 421 CGGGTCGTGG GTCATCCTGC TGCCAATCCA GCTGCTGCAA GCCCTGCTGC TCATCCTCAG 481 GCTGTGGGTC ATCCTGCTGC CAGTCCAGCT GCTGCAAGCC CTGCTGCTCC CAGTCCAGAT 541 GCTGTGTCCC TGTGTGCTAC CAGTGCAAGA TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 721 TCGCAAATAC TACCTAGATC GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt