Construct: ORF TRCN0000473594
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003672.1_s317c1
- Derived from:
- ccsbBroadEn_06598
- DNA Barcode:
- TGCAAGCTAGAGCAGCCGCGTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT1A (4489)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473594
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 99.4% | 98.3% | 80C>A |
| 2 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 93.9% | 91.8% | (many diffs) |
| 3 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 92.8% | 88.5% | (many diffs) |
| 4 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 92.8% | 86.8% | (many diffs) |
| 5 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 91.8% | 88.5% | (many diffs) |
| 6 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 91.3% | 87% | (many diffs) |
| 7 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 91.2% | 85.2% | (many diffs) |
| 8 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 91.2% | 85.2% | (many diffs) |
| 9 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 91.2% | 83.6% | (many diffs) |
| 10 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 90.7% | 80.3% | (many diffs) |
| 11 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 89.6% | 83.6% | (many diffs) |
| 12 | human | 4494 | MT1F | metallothionein 1F | NM_001301272.1 | 63.4% | 49.2% | (many diffs) |
| 13 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 44.4% | (many diffs) | |
| 14 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 41.4% | (many diffs) | |
| 15 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 38.3% | (many diffs) | |
| 16 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 36.2% | (many diffs) | |
| 17 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_104045.1 | 32.6% | (many diffs) | |
| 18 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_003658.2 | 31.5% | (many diffs) | |
| 19 | mouse | 17750 | Mt2 | metallothionein 2 | NM_008630.2 | 87.4% | 80.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 247
- ORF end:
- 430
- ORF length:
- 183
- Sequence:
-
1 GGAGTACGTC GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG 61 AGTGGGTGGA GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG 121 CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT 181 TTCTTCCATT TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA 241 GTTGGCATGG ACCCCAACTG CTCCTGCGCC ACTGGTGGCT CCTGCACCTG CACTGGCTCC 301 TGCAAATGCA AAGAGTGCAA ATGCAACTCC TGCAAGAAGA GCTGCTGCTC CTGCTGCCCC 361 ATGAGCTGTG CCAAGTGTGC CCAGGGCTGC ATCTGCAAAG GGGCATCAGA GAAGTGCAGC 421 TGCTGTGCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC 481 CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG 541 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGCAA GCTAGAGCAG CCGCGTATGA 601 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt