Construct: ORF TRCN0000473796
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018869.3_s317c1
- Derived from:
- ccsbBroadEn_15307
- DNA Barcode:
- AACCCCACCAACCTATGGAACTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NEK5 (341676)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473796
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 341676 | NEK5 | NIMA related kinase 5 | NM_199289.3 | 95.6% | 36% | (many diffs) |
| 2 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020555.1 | 89.7% | 33.7% | (many diffs) |
| 3 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020557.1 | 86.8% | 32.6% | (many diffs) |
| 4 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_017020556.1 | 83.3% | 34.5% | (many diffs) |
| 5 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_011535066.2 | 81.6% | 36.5% | (many diffs) |
| 6 | human | 341676 | NEK5 | NIMA related kinase 5 | NM_001365552.1 | 80.6% | 30.6% | (many diffs) |
| 7 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_941575.2 | 74.4% | (many diffs) | |
| 8 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_006719808.4 | 67.9% | 42.6% | (many diffs) |
| 9 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_941574.2 | 64.4% | (many diffs) | |
| 10 | human | 341676 | NEK5 | NIMA related kinase 5 | XM_011535068.2 | 53.8% | 52.7% | (many diffs) |
| 11 | human | 341676 | NEK5 | NIMA related kinase 5 | XR_001749542.1 | 48.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 861
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggataagtac gatgtgatta aggccatcgg gcaaggtgcc ttcgggaaag 121 catacttagc taaagggaaa tcagatagca agcactgtgt cataaaagag atcaattttg 181 aaaagatgcc catacaagaa aaagaagctt caaagaaaga agtgattctt ctggaaaaga 241 tgaaacatcc caacattgta gccttcttca attcatttca agagaatggc aggctgttta 301 ttgtaatgga atattgtgat ggaggggatc tcatgaaaag gatcaataga caacggggtg 361 tgttatttag tgaagatcag atcctcggtt ggtttgtaca gatttctcta ggactaaaac 421 atattcatga caggaagata ttacacaggg acataaaagc tcagaacatt tttcttagca 481 agaacggaat ggtggcaaag cttggggact ttggtatagc aagagtcctg aataattcca 541 tggaacttgc tcgaacttgt attggaacac cttactacct gtccccagag atctgtcaga 601 ataaacccta caacaataaa acggatattt ggtctcttgg ctgtgtctta tatgagctct 661 gcacacttaa acatcctttt gagggtaaca acttacagca gctggttctg aagatttgtc 721 aagcacattt tgccccaata tctccggggt tttctcgtga gctccattcc ttgatatctc 781 agctctttca agtatctctc gagaccgaca tcataaattc cattttgaaa gcccttttag 841 agaatcttat tcccaatatt tgactcctgg aggtcattca ggaggaattc agtcacatgc 901 ttatatgcag agcaggagcg ccagcttctc gacatgctgg gaaggtggtc cagaagtgta 961 aaatacaaaa agtgagattc cagggaaagt gcccaccaag atcaaggata tctgtgccaa 1021 ttaaaaggaa tgctatattg catagaaatg aatggagacc accagctgga gcccagaagg 1081 ccagatctat aaaaatgata gaaagaccca aaattgctgc tgtctgtgga cattatgatt 1141 attattatgc tcaacttgat atgctgagga ggagagccca caaaccaagt tatcacccta 1201 ttcctcaaga aaatactgga gttgaggatt acggtcagga aacgaggcat ggtccatccc 1261 caagtcaatg gcctgctgag taccttcaga gaaaatttga aggtccacaa tataagtttg 1321 aaagtggaga agcaattggg tcttcgtcca tctcttgccg agccaaatta caaccaagag 1381 acaagagcta gnaagtaatg gagaagagcc tagattccag gagctgccat ttaggaaaaa 1441 cgaaatgaag gaacaggaat attggaagca gttagaggaa atacgccaac agtaccacaa 1501 tgacatgaaa gaaattagaa agaagatggg gagagaacca gaggagaact caaaaataag 1561 tcataaaacc tatttggtga agaagagtaa cctgcctgtc catcaagatg catctgaggg 1621 agaagcacct gtgcaggaca ttgaaaaaga cttgaaacaa atgaggcttc agaacacaaa 1681 ggaaagtaaa aatccagaaC AGAAATATAA AGCTAAGAAG GGGGTAAAAT TTGAAATTAA 1741 TTTAGACAAA TGTATTTCTG ATGAAAACAT CCTCCAAGAG GAAGAGGCAA TGGATATACC 1801 AAATGAAACT TTGACCTTTG AGGATGGCAT GAAGTTTAAG GAATATGAAT GTGTAAAGGA 1861 GCATGGAGAT TATACAGACA AAGCATTTGA AAAACTTCAC TGCCCAGAAG CAGGGTTTTC 1921 CACGCAGACT GTAGCTGCTG TGGGAAACAG GAGGCAGTGG GATGGAGGAG CGCCTCAGAC 1981 TCTGCTGCAG ATGATGGCAG TGGCCGACAT CACCTCCACC TGCCCCACGG GGCCTGACAG 2041 TGAGTCTGTG CTTAGCGTCA GTCGTCAGGA AGGGAAGACC AAGGACCCGT ACAGCCCAGT 2101 GCTCATCCTG ATGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 2161 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 2221 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ACCCCACCAA CCTATGGAAC 2281 TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att