Construct: ORF TRCN0000473870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002286.1_s317c1
- Derived from:
- ccsbBroadEn_03303
- DNA Barcode:
- TCGCCAAGCTGGTAATCACGGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKAG2 (51422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_001304531.1 | 100% | 100% | |
| 2 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_024429.1 | 100% | 100% | |
| 3 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_024446789.1 | 100% | 100% | |
| 4 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012276.2 | 99.6% | 99.6% | 29_30insAGC |
| 5 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012277.2 | 97.3% | 96.9% | 0_1ins22;2T>G;6_6delCinsGAA |
| 6 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012278.1 | 93.9% | 93.9% | 0_1ins60 |
| 7 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012279.2 | 93.9% | 93.9% | 0_1ins60 |
| 8 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012280.2 | 93.9% | 93.9% | 0_1ins60 |
| 9 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012281.2 | 93.9% | 93.9% | 0_1ins60 |
| 10 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516285.2 | 85.1% | 83.9% | (many diffs) |
| 11 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_024446787.1 | 85.1% | 83.9% | (many diffs) |
| 12 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012274.2 | 84.9% | 83.7% | (many diffs) |
| 13 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_024446788.1 | 84.9% | 83.7% | (many diffs) |
| 14 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516286.2 | 82.9% | 81.3% | (many diffs) |
| 15 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516287.1 | 79.9% | 78.7% | (many diffs) |
| 16 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012275.2 | 79.9% | 78.7% | (many diffs) |
| 17 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_001363698.1 | 73.7% | 73.7% | 1_351del |
| 18 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_001304527.1 | 73.4% | 73.4% | 1_351del;380_381insAGC |
| 19 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_005250006.5 | 65.1% | 64.2% | (many diffs) |
| 20 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_001040633.1 | 62.4% | 62.4% | 1_591del |
| 21 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012270.1 | 62.4% | 62.4% | 1_591del |
| 22 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012271.2 | 62.2% | 62.2% | 1_591del;620_621insAGC |
| 23 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012272.1 | 62.2% | 62.2% | 1_591del;620_621insAGC |
| 24 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516283.1 | 58% | 58% | 1_711del |
| 25 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516284.1 | 57.8% | 57.8% | 1_711del;740_741insAGC |
| 26 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_016203.4 | 57.6% | 57.6% | 1_723del |
| 27 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012269.1 | 57.4% | 57.4% | 1_723del;752_753insAGC |
| 28 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_005250004.4 | 56.1% | 55.3% | (many diffs) |
| 29 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_024446786.1 | 56.1% | 55.3% | (many diffs) |
| 30 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012268.2 | 55.9% | 55.1% | (many diffs) |
| 31 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_006716021.2 | 52.4% | 51.7% | (many diffs) |
| 32 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516282.1 | 52.3% | 51.6% | (many diffs) |
| 33 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_005250002.4 | 52.1% | 51.4% | (many diffs) |
| 34 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | NM_001170555.1 | 91.7% | 98.4% | (many diffs) |
| 35 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | NM_001170556.1 | 91.4% | 98.1% | (many diffs) |
| 36 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_011249740.1 | 90% | 96.3% | (many diffs) |
| 37 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_017320596.1 | 89.8% | 96% | (many diffs) |
| 38 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_006535614.3 | 72.3% | 77.6% | (many diffs) |
| 39 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | NM_001310480.1 | 67.7% | 72.7% | (many diffs) |
| 40 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_006535612.3 | 57.4% | 61.6% | (many diffs) |
| 41 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_006535611.3 | 52.9% | 56.8% | (many diffs) |
| 42 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | NM_145401.2 | 52.8% | 56.6% | (many diffs) |
| 43 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_017320595.1 | 40.5% | 43.4% | (many diffs) |
| 44 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XR_880821.2 | 24% | (many diffs) | |
| 45 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XR_389738.3 | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1050
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggagaagctg gagttcgagg acgaagcagt agaagactca gaaagtggtg 121 tttacatgcg attcatgagg tcacacaagt gttatgacat cgttccaacc agttcaaagc 181 ttgttgtctt tgatactaca ttacaagtta aaaaggcctt ctttgctttg gtagccaacg 241 gtgtccgagc agcgccactg tgggagagta aaaaacaaag ttttgtagga atgctaacaa 301 ttacagattt cataaatata ctacatagat actataaatc acctatggta cagatttatg 361 aattagagga acataaaatt gaaacatgga gggagcttta tttacaagaa acatttaagc 421 ctttagtgaa tatatctcca gatgcaagcc tcttcgatgc tgtatactcc ttgatcaaaa 481 ataaaatcca cagattgccc gttattgacc ctatcagtgg gaatgcactt tatatactta 541 cccacaaaag aatcctcaag ttcctccagc tttttatgtc tgatatgcca aagcctgcct 601 tcatgaagca gaaccTGGAT GAGCTTGGAA TAGGAACGTA CCACAACATT GCCTTCATAC 661 ATCCAGACAC TCCCATCATC AAAGCCTTGA ACATATTTGT GGAAAGACGA ATATCAGCTC 721 TGCCTGTTGT GGATGAGTCA GGAAAAGTTG TAGATATTTA TTCCAAATTT GATGTAATTA 781 ATCTTGCTGC TGAGAAAACA TACAATAACC TAGATATCAC GGTGACCCAG GCCCTTCAGC 841 ACCGTTCACA GTATTTTGAA GGTGTTGTGA AGTGCAATAA GCTGGAAATA CTGGAGACCA 901 TCGTGGACAG AATAGTAAGA GCTGAGGTCC ATCGGCTGGT GGTGGTAAAT GAAGCAGATA 961 GTATTGTGGG TATTATTTCC CTGTCGGACA TTCTGCAAGC CCTGATCCTC ACACCAGCAG 1021 GTGCCAAACA AAAGGAGACA GAAACGGAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCGCC 1201 AAGCTGGTAA TCACGGCAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt