Transcript: Human NM_001330264.1

Homo sapiens TYRO3 protein tyrosine kinase (TYRO3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
TYRO3 (7301)
Length:
3966
CDS:
337..2874

Additional Resources:

NCBI RefSeq record:
NM_001330264.1
NBCI Gene record:
TYRO3 (7301)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147880 CCTACCTTGAAGGTGAACAG pXPR_003 TGG 520 20% 5 0.6351 TYRO3 TYRO3 76227
2 BRDN0001146050 GGAGTTTGACCATCCACACG pXPR_003 TGG 1600 63% 14 0.1869 TYRO3 TYRO3 76228
3 BRDN0001147403 TGCGCTGTGCCAATGCCTTG pXPR_003 GGG 774 30% 7 -0.3651 TYRO3 TYRO3 76229
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001330264.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000379716 GGGCATCAGCGATGAACTAAA pLKO_005 1695 CDS 100% 13.200 18.480 N TYRO3 n/a
2 TRCN0000231524 CCGGTCCTTCAATCGAGAAAG pLKO_005 1641 CDS 100% 10.800 15.120 N TYRO3 n/a
3 TRCN0000199533 GCCCTCATCCTGCTTCGAAAG pLKO.1 1540 CDS 100% 2.000 2.800 N TYRO3 n/a
4 TRCN0000231527 CCAGTGACTGTCGGTACATAC pLKO_005 2732 CDS 100% 10.800 8.640 N TYRO3 n/a
5 TRCN0000195673 CCCTGAACCTGTTACCATTGT pLKO.1 696 CDS 100% 4.950 3.960 N TYRO3 n/a
6 TRCN0000195244 CAAAGAGATGTCCTTGTAATA pLKO.1 3339 3UTR 100% 13.200 9.240 N TYRO3 n/a
7 TRCN0000231526 AGTGTATGGAGGACGTGTATG pLKO_005 2468 CDS 100% 10.800 7.560 N TYRO3 n/a
8 TRCN0000381303 TTCAACATCACCGTGACAAAG pLKO_005 886 CDS 100% 10.800 7.560 N TYRO3 n/a
9 TRCN0000231528 TTGGTATCTCAGGTCTGAATC pLKO_005 3389 3UTR 100% 10.800 7.560 N TYRO3 n/a
10 TRCN0000197199 GCTGCTTAAGTGCATGCATTG pLKO.1 3244 3UTR 100% 6.000 4.200 N TYRO3 n/a
11 TRCN0000195586 CCCTTCTAAACGGTGACCTTT pLKO.1 3705 3UTR 100% 4.950 3.465 N TYRO3 n/a
12 TRCN0000002181 CCTTCTCCATACCCACAATCT pLKO.1 3622 3UTR 100% 4.950 3.465 N TYRO3 n/a
13 TRCN0000002178 GAGTGTATGGAGGACGTGTAT pLKO.1 2467 CDS 100% 4.950 3.465 N TYRO3 n/a
14 TRCN0000002179 GTGGAGAGGAACTACGAAGAT pLKO.1 720 CDS 100% 4.950 3.465 N TYRO3 n/a
15 TRCN0000199890 GCCAGCTGTCTGTGCTATCTG pLKO.1 2573 CDS 100% 1.650 1.155 N TYRO3 n/a
16 TRCN0000231525 TCATTGCCTCAAGCGACATTG pLKO_005 1868 CDS 100% 10.800 6.480 N TYRO3 n/a
17 TRCN0000002180 CGAGCTTTACTTGTCTGCGAA pLKO.1 2531 CDS 100% 2.640 1.584 N TYRO3 n/a
18 TRCN0000010684 CGCTGAGATTTACAACTACCT pLKO.1 2415 CDS 100% 2.640 1.584 N TYRO3 n/a
19 TRCN0000023526 GCAGCTTGCATGAAGGAGTTT pLKO.1 1906 CDS 100% 4.950 2.475 Y Tyro3 n/a
20 TRCN0000023528 GCGAATGGAACTGGAGAACAT pLKO.1 2547 CDS 100% 4.950 2.475 Y Tyro3 n/a
21 TRCN0000361566 TCATTGCCTCAAGCGACATAG pLKO_005 1868 CDS 100% 10.800 6.480 N Tyro3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330264.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14871 pDONR223 0% 94.9% 94.9% None 0_1ins135 n/a
2 ccsbBroad304_14871 pLX_304 0% 94.9% 94.9% V5 0_1ins135 n/a
3 TRCN0000479963 TCCTTCCTGGCGGTACGGTCCTAA pLX_317 13.4% 94.8% 94.7% V5 0_1ins135;2320C>A;2335A>G n/a
4 ccsbBroadEn_15617 pDONR223 0% 94.9% 94.9% None 0_1ins135;612T>C n/a
5 ccsbBroad304_15617 pLX_304 0% 94.9% 94.9% V5 0_1ins135;612T>C n/a
6 TRCN0000488607 GTCTTAGTGAACTGCAAAAATAAT pLX_317 14.6% 94.7% 94.6% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000489876 GCATTCACCTTCAGGTGAGCCTTG pLX_317 15.5% 94.7% 94.5% V5 (many diffs) n/a
8 ccsbBroadEn_13975 pDONR223 100% 94.7% 94.2% None (many diffs) n/a
9 ccsbBroad304_13975 pLX_304 0% 94.7% 94.2% V5 (not translated due to frame shift) (many diffs) n/a
10 TRCN0000474009 TGCGGAACTACTAATCCGCATTTC pLX_317 9.6% 94.7% 94.2% V5 (not translated due to frame shift) (many diffs) n/a
11 TRCN0000487789 GGTACCGAAATCCGACTACACCGA pLX_317 21.1% 49.9% 49.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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