Transcript: Mouse XM_006499160.1

PREDICTED: Mus musculus TYRO3 protein tyrosine kinase 3 (Tyro3), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tyro3 (22174)
Length:
3755
CDS:
148..2685

Additional Resources:

NCBI RefSeq record:
XM_006499160.1
NBCI Gene record:
Tyro3 (22174)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146050 GGAGTTTGACCATCCACACG pXPR_003 TGG 1600 63% 14 0.2289 Tyro3 TYRO3 76228
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006499160.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361494 CTTGTGAAGCCCGCAACATAA pLKO_005 617 CDS 100% 13.200 18.480 N Tyro3 n/a
2 TRCN0000361563 AGACCTAGCAGCTCGGAATTG pLKO_005 1974 CDS 100% 10.800 15.120 N Tyro3 n/a
3 TRCN0000023528 GCGAATGGAACTGGAGAACAT pLKO.1 2358 CDS 100% 4.950 6.930 N Tyro3 n/a
4 TRCN0000023525 GCCCGATCTTTCAATCGAGAA pLKO.1 1450 CDS 100% 4.050 5.670 N Tyro3 n/a
5 TRCN0000023527 CGCCATATGCTGGCATTGAAA pLKO.1 2204 CDS 100% 0.000 0.000 N Tyro3 n/a
6 TRCN0000361564 GGCTGAGCTGCTCCTACTTTA pLKO_005 3035 3UTR 100% 13.200 9.240 N Tyro3 n/a
7 TRCN0000361566 TCATTGCCTCAAGCGACATAG pLKO_005 1679 CDS 100% 10.800 7.560 N Tyro3 n/a
8 TRCN0000023526 GCAGCTTGCATGAAGGAGTTT pLKO.1 1717 CDS 100% 4.950 3.465 N Tyro3 n/a
9 TRCN0000023524 CCTCAGAATTTCCATGCCATT pLKO.1 988 CDS 100% 4.050 2.835 N Tyro3 n/a
10 TRCN0000231525 TCATTGCCTCAAGCGACATTG pLKO_005 1679 CDS 100% 10.800 7.560 N TYRO3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006499160.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14871 pDONR223 0% 83.3% 85.1% None (many diffs) n/a
2 ccsbBroad304_14871 pLX_304 0% 83.3% 85.1% V5 (many diffs) n/a
3 TRCN0000479963 TCCTTCCTGGCGGTACGGTCCTAA pLX_317 13.4% 83.3% 85.2% V5 (many diffs) n/a
4 ccsbBroadEn_15617 pDONR223 0% 83.3% 85.1% None (many diffs) n/a
5 ccsbBroad304_15617 pLX_304 0% 83.3% 85.1% V5 (many diffs) n/a
6 TRCN0000488607 GTCTTAGTGAACTGCAAAAATAAT pLX_317 14.6% 83.2% 85.1% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000489876 GCATTCACCTTCAGGTGAGCCTTG pLX_317 15.5% 83.1% 85% V5 (many diffs) n/a
8 ccsbBroadEn_13975 pDONR223 100% 83.1% 84.8% None (many diffs) n/a
9 ccsbBroad304_13975 pLX_304 0% 83.1% 84.8% V5 (not translated due to frame shift) (many diffs) n/a
10 TRCN0000474009 TGCGGAACTACTAATCCGCATTTC pLX_317 9.6% 83.1% 84.8% V5 (not translated due to frame shift) (many diffs) n/a
11 TRCN0000487789 GGTACCGAAATCCGACTACACCGA pLX_317 21.1% 44.8% 46.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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