Construct: ORF TRCN0000474296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011228.1_s317c1
- Derived from:
- ccsbBroadEn_07222
- DNA Barcode:
- CCGAGAACTTAGACACGTTCATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AC14 (8331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474296
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8331 | H2AC14 | H2A clustered histone 14 | NM_021066.2 | 99.7% | 100% | 150G>A |
| 2 | human | 8329 | H2AC13 | H2A clustered histone 13 | NM_003509.2 | 96.4% | 97.6% | (many diffs) |
| 3 | human | 85235 | H2AC12 | H2A clustered histone 12 | NM_080596.2 | 90.3% | 99.2% | (many diffs) |
| 4 | human | 8969 | H2AC11 | H2A clustered histone 11 | NM_021064.4 | 89.7% | 97.6% | (many diffs) |
| 5 | human | 8330 | H2AC15 | H2A clustered histone 15 | NM_003510.2 | 88.7% | 97.6% | (many diffs) |
| 6 | human | 8332 | H2AC16 | H2A clustered histone 16 | NM_003511.2 | 88.2% | 97.6% | (many diffs) |
| 7 | human | 8336 | H2AC17 | H2A clustered histone 17 | NM_003514.2 | 86.6% | 97.6% | (many diffs) |
| 8 | human | 8334 | H2AC6 | H2A clustered histone 6 | NM_003512.3 | 85.8% | 96.1% | (many diffs) |
| 9 | human | 723790 | H2AC19 | H2A clustered histone 19 | NM_001040874.1 | 85.3% | 96.1% | (many diffs) |
| 10 | human | 8337 | H2AC18 | H2A clustered histone 18 | NM_003516.2 | 85.3% | 96.1% | (many diffs) |
| 11 | human | 92815 | H2AW | H2A.W histone | NM_033445.2 | 85.1% | 95.3% | (many diffs) |
| 12 | human | 8338 | H2AC20 | H2A clustered histone 20 | NM_003517.2 | 85% | 96.1% | (many diffs) |
| 13 | human | 3013 | H2AC7 | H2A clustered histone 7 | NM_021065.3 | 83.3% | 96.9% | (many diffs) |
| 14 | human | 8335 | H2AC4 | H2A clustered histone 4 | NM_003513.2 | 83.3% | 96.1% | (many diffs) |
| 15 | human | 3012 | H2AC8 | H2A clustered histone 8 | NM_021052.2 | 82.3% | 96.1% | (many diffs) |
| 16 | human | 55766 | H2AJ | H2A.J histone | NM_177925.3 | 81.7% | 95.3% | (many diffs) |
| 17 | human | 317772 | H2AC21 | H2A clustered histone 21 | NM_175065.2 | 80.2% | 93.8% | (many diffs) |
| 18 | human | 3014 | H2AX | H2A.X variant histone | NM_002105.2 | 73.6% | 82.5% | (many diffs) |
| 19 | mouse | 319168 | Hist1h2ah | histone cluster 1, H2ah | NM_175659.2 | 89% | 97.6% | (many diffs) |
| 20 | mouse | 319166 | Hist1h2ae | histone cluster 1, H2ae | NM_178187.4 | 87.9% | 96.1% | (many diffs) |
| 21 | mouse | 319164 | Hist1h2ac | histone cluster 1, H2ac | NM_178189.5 | 87.6% | 96.1% | (many diffs) |
| 22 | mouse | 665433 | Hist1h2ao | histone cluster 1, H2ao | NM_001177544.2 | 87.4% | 96.1% | (many diffs) |
| 23 | mouse | 319172 | Hist1h2ab | histone cluster 1, H2ab | NM_175660.3 | 87.4% | 96.1% | (many diffs) |
| 24 | mouse | 319171 | Hist1h2ap | histone cluster 1, H2ap | NM_178185.2 | 87.4% | 96.1% | (many diffs) |
| 25 | mouse | 15267 | Hist2h2aa1 | histone cluster 2, H2aa1 | NM_013549.2 | 87.1% | 96.1% | (many diffs) |
| 26 | mouse | 319191 | Hist1h2ai | histone cluster 1, H2ai | NM_178182.2 | 87.1% | 96.1% | (many diffs) |
| 27 | mouse | 319167 | Hist1h2ag | histone cluster 1, H2ag | NM_178186.3 | 87.1% | 96.1% | (many diffs) |
| 28 | mouse | 319165 | Hist1h2ad | histone cluster 1, H2ad | NM_178188.4 | 87.1% | 96.1% | (many diffs) |
| 29 | mouse | 319192 | Hist2h2aa2 | histone cluster 2, H2aa2 | NM_178212.3 | 87.1% | 96.1% | (many diffs) |
| 30 | mouse | 319170 | Hist1h2an | histone cluster 1, H2an | NM_178184.2 | 86.9% | 96.1% | (many diffs) |
| 31 | mouse | 319176 | Hist2h2ac | histone cluster 2, H2ac | NM_175662.2 | 86.9% | 96.1% | (many diffs) |
| 32 | mouse | 319173 | Hist1h2af | histone cluster 1, H2af | NM_175661.2 | 86.6% | 96.1% | (many diffs) |
| 33 | mouse | 319169 | Hist1h2ak | histone cluster 1, H2ak | NM_178183.2 | 86.1% | 95.3% | (many diffs) |
| 34 | mouse | 232440 | H2afj | H2A histone family, member J | NM_177688.4 | 84.4% | 94.5% | (many diffs) |
| 35 | mouse | 319162 | Hist3h2a | histone cluster 3, H2a | NM_178218.4 | 83.5% | 95.3% | (many diffs) |
| 36 | mouse | 319163 | Hist1h2aa | histone cluster 1, H2aa | NM_175658.2 | 80.6% | 89.1% | (many diffs) |
| 37 | mouse | 621893 | Hist2h2ab | histone cluster 2, H2ab | NM_178213.4 | 79.6% | 93% | (many diffs) |
| 38 | mouse | 15270 | H2afx | H2A histone family, member X | NM_010436.2 | 76.1% | 81.8% | (many diffs) |
| 39 | mouse | 667728 | Hist1h2al | histone cluster 1, H2al | NR_132443.1 | 10.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGTC TGGGCGTGGT AAGCAGGGAG GCAAAGCTCG CGCCAAGGCC AAGACCCGCT 121 CTTCTCGGGC CGGGCTTCAG TTTCCCGTAG GCCGAGTGCA TCGCCTGCTC CGCAAAGGCA 181 ACTATGCGGA GCGGGTCGGT GCTGGAGCGC CGGTATACCT GGCGGCGGTG CTGGAGTACC 241 TGACCGCCGA GATCCTGGAG CTGGCTGGCA ACGCGGCCCG CGACAACAAG AAGACTCGCA 301 TCATCCCGCG TCACCTCCAG CTGGCCATCC GCAACGATGA GGAGCTCAAC AAGCTTCTGG 361 GCAAAGTCAC CATCGCACAG GGTGGCGTCC TGCCCAACAT CCAGGCCGTG CTGCTGCCAA 421 AGAAAACTGA GAGCCACCAC AAGACTAAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCGAG 601 AACTTAGACA CGTTCATCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt