Construct: ORF TRCN0000474578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002577.1_s317c1
- Derived from:
- ccsbBroadEn_02510
- DNA Barcode:
- CGTCGATTGGCGGAGATGTTTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNPY3 (10695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NM_006586.5 | 100% | 100% | |
| 2 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NM_001318842.1 | 89.3% | 89.3% | 276_374del |
| 3 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NM_001318845.1 | 67.6% | 66.9% | (many diffs) |
| 4 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NM_001318848.2 | 51.3% | 45.5% | (many diffs) |
| 5 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NR_134880.1 | 45.1% | 1_441del;717_778del;1338_1846del | |
| 6 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NR_134882.1 | 42.4% | (many diffs) | |
| 7 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NM_001318847.2 | 38.7% | 32.8% | (many diffs) |
| 8 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NR_134888.1 | 32.9% | (many diffs) | |
| 9 | human | 10695 | CNPY3 | canopy FGF signaling regula... | NR_134881.1 | 27.4% | (many diffs) | |
| 10 | mouse | 72029 | Cnpy3 | canopy FGF signaling regula... | NM_028065.4 | 86.9% | 88.1% | (many diffs) |
| 11 | mouse | 72029 | Cnpy3 | canopy FGF signaling regula... | NM_001305988.1 | 52.9% | 54.3% | (many diffs) |
| 12 | mouse | 72029 | Cnpy3 | canopy FGF signaling regula... | NM_001305989.1 | 48.1% | 48.2% | (many diffs) |
| 13 | mouse | 72029 | Cnpy3 | canopy FGF signaling regula... | NM_001305990.1 | 48.1% | 48.2% | (many diffs) |
| 14 | mouse | 72029 | Cnpy3 | canopy FGF signaling regula... | XM_017317654.1 | 48.1% | 48.2% | (many diffs) |
| 15 | mouse | 72029 | Cnpy3 | canopy FGF signaling regula... | XR_001782114.1 | 36.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 900
- ORF length:
- 834
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttcaatgcct gagcccgcgt cccgctgtct tctgcttctt cccttgctgc 121 tgctgctgct gctgctgctg ccggccccgg agctgggccc gagccaggcc ggagctgagg 181 agaacgactg ggttcgcctg cccagcaaat gcgaagtgtg taaatatgtt gctgtggagc 241 tgaagtcagc ctttgaggaa accggcaaga ccaaggaggt gattggcacg ggctatggca 301 tcctggacca gaaggcctct ggagtcaaat acaccaagtc ggacttgcgg ttaatcgaag 361 tcactgagac catttgcaag aggctcctgg attatagcct gcacaaggag aggaccggca 421 gcaatcgatt tgccaagggc atgtcagaga cctttgagac attacacaac ctggtacaca 481 aaggggtcaa ggtggtgatg gacatcccct atgagctgtg gaacgagact tctgcagagg 541 tggctgacct caagaagcag tgtgatgtgc tggtggaaga gtttgaggag gtgatcgagg 601 actggtacag gaaccaccag gaggaagacc tgactgaatt cctcTGCGCC AACCACGTGC 661 TGAAGGGAAA AGACACCAGT TGCCTGGCAG AGCAGTGGTC CGGCAAGAAG GGAGACACAG 721 CTGCCCTGGG AGGGAAGAAG TCCAAGAAGA AGAGCAGCAG GGCCAAGGCA GCAGGCGGCA 781 GGAGTAGCAG CAGCAAACAA AGGAAGGAGC TGGGTGGCCT TGAGGGAGAC CCCAGCCCCG 841 AGGAGGATGA GGGCATCCAG AAGGCATCCC CTCTCACACA CAGCCCCCCT GATGAGCTCT 901 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 961 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1021 TTTATATATC TTGTGGAAAG GACGACGTCG ATTGGCGGAG ATGTTTTCTA CGCGTTAAGT 1081 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt