Construct: ORF TRCN0000474646
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000725.1_s317c1
- Derived from:
- ccsbBroadEn_15628
- DNA Barcode:
- TCTACAGTCTCGTAGCTATGTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AC14 (8331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474646
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8331 | H2AC14 | H2A clustered histone 14 | NM_021066.2 | 100% | 100% | |
| 2 | human | 8329 | H2AC13 | H2A clustered histone 13 | NM_003509.2 | 96.6% | 97.6% | (many diffs) |
| 3 | human | 85235 | H2AC12 | H2A clustered histone 12 | NM_080596.2 | 90.6% | 99.2% | (many diffs) |
| 4 | human | 8969 | H2AC11 | H2A clustered histone 11 | NM_021064.4 | 90% | 97.6% | (many diffs) |
| 5 | human | 8330 | H2AC15 | H2A clustered histone 15 | NM_003510.2 | 88.9% | 97.6% | (many diffs) |
| 6 | human | 8332 | H2AC16 | H2A clustered histone 16 | NM_003511.2 | 88.4% | 97.6% | (many diffs) |
| 7 | human | 8336 | H2AC17 | H2A clustered histone 17 | NM_003514.2 | 86.6% | 97.6% | (many diffs) |
| 8 | human | 8334 | H2AC6 | H2A clustered histone 6 | NM_003512.3 | 86.1% | 96.1% | (many diffs) |
| 9 | human | 723790 | H2AC19 | H2A clustered histone 19 | NM_001040874.1 | 85.3% | 96.1% | (many diffs) |
| 10 | human | 8337 | H2AC18 | H2A clustered histone 18 | NM_003516.2 | 85.3% | 96.1% | (many diffs) |
| 11 | human | 92815 | H2AW | H2A.W histone | NM_033445.2 | 85.1% | 95.3% | (many diffs) |
| 12 | human | 8338 | H2AC20 | H2A clustered histone 20 | NM_003517.2 | 85% | 96.1% | (many diffs) |
| 13 | human | 3013 | H2AC7 | H2A clustered histone 7 | NM_021065.3 | 83.5% | 96.9% | (many diffs) |
| 14 | human | 8335 | H2AC4 | H2A clustered histone 4 | NM_003513.2 | 83.5% | 96.1% | (many diffs) |
| 15 | human | 3012 | H2AC8 | H2A clustered histone 8 | NM_021052.2 | 82.5% | 96.1% | (many diffs) |
| 16 | human | 55766 | H2AJ | H2A.J histone | NM_177925.3 | 82% | 95.3% | (many diffs) |
| 17 | human | 317772 | H2AC21 | H2A clustered histone 21 | NM_175065.2 | 80.5% | 93.8% | (many diffs) |
| 18 | human | 221613 | H2AC1 | H2A clustered histone 1 | NM_170745.3 | 77.7% | 90.8% | (many diffs) |
| 19 | human | 3014 | H2AX | H2A.X variant histone | NM_002105.2 | 73.9% | 82.5% | (many diffs) |
| 20 | mouse | 319168 | Hist1h2ah | histone cluster 1, H2ah | NM_175659.2 | 89.3% | 97.6% | (many diffs) |
| 21 | mouse | 319166 | Hist1h2ae | histone cluster 1, H2ae | NM_178187.4 | 88.2% | 96.1% | (many diffs) |
| 22 | mouse | 319172 | Hist1h2ab | histone cluster 1, H2ab | NM_175660.3 | 87.6% | 96.1% | (many diffs) |
| 23 | mouse | 319164 | Hist1h2ac | histone cluster 1, H2ac | NM_178189.5 | 87.6% | 96.1% | (many diffs) |
| 24 | mouse | 665433 | Hist1h2ao | histone cluster 1, H2ao | NM_001177544.2 | 87.4% | 96.1% | (many diffs) |
| 25 | mouse | 319191 | Hist1h2ai | histone cluster 1, H2ai | NM_178182.2 | 87.4% | 96.1% | (many diffs) |
| 26 | mouse | 319171 | Hist1h2ap | histone cluster 1, H2ap | NM_178185.2 | 87.4% | 96.1% | (many diffs) |
| 27 | mouse | 319165 | Hist1h2ad | histone cluster 1, H2ad | NM_178188.4 | 87.4% | 96.1% | (many diffs) |
| 28 | mouse | 319170 | Hist1h2an | histone cluster 1, H2an | NM_178184.2 | 87.1% | 96.1% | (many diffs) |
| 29 | mouse | 319167 | Hist1h2ag | histone cluster 1, H2ag | NM_178186.3 | 87.1% | 96.1% | (many diffs) |
| 30 | mouse | 15267 | Hist2h2aa1 | histone cluster 2, H2aa1 | NM_013549.2 | 86.9% | 96.1% | (many diffs) |
| 31 | mouse | 319173 | Hist1h2af | histone cluster 1, H2af | NM_175661.2 | 86.9% | 96.1% | (many diffs) |
| 32 | mouse | 319192 | Hist2h2aa2 | histone cluster 2, H2aa2 | NM_178212.3 | 86.9% | 96.1% | (many diffs) |
| 33 | mouse | 319176 | Hist2h2ac | histone cluster 2, H2ac | NM_175662.2 | 86.6% | 96.1% | (many diffs) |
| 34 | mouse | 319169 | Hist1h2ak | histone cluster 1, H2ak | NM_178183.2 | 86.4% | 95.3% | (many diffs) |
| 35 | mouse | 232440 | H2afj | H2A histone family, member J | NM_177688.4 | 84.7% | 94.5% | (many diffs) |
| 36 | mouse | 319162 | Hist3h2a | histone cluster 3, H2a | NM_178218.4 | 83.8% | 95.3% | (many diffs) |
| 37 | mouse | 319163 | Hist1h2aa | histone cluster 1, H2aa | NM_175658.2 | 80.8% | 89.1% | (many diffs) |
| 38 | mouse | 621893 | Hist2h2ab | histone cluster 2, H2ab | NM_178213.4 | 79.8% | 93% | (many diffs) |
| 39 | mouse | 15270 | H2afx | H2A histone family, member X | NM_010436.2 | 76.3% | 81.8% | (many diffs) |
| 40 | mouse | 667728 | Hist1h2al | histone cluster 1, H2al | NR_132443.1 | 10.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 474
- ORF length:
- 384
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTGGGCG TGGTAAGCAG GGAGGCAAAG 121 CTCGCGCCAA GGCCAAGACC CGCTCTTCTC GGGCCGGGCT TCAGTTTCCC GTAGGCCGAG 181 TGCATCGCCT GCTCCGCAAA GGCAACTATG CGGAGCGGGT CGGTGCTGGA GCGCCGGTGT 241 ACCTGGCGGC GGTGCTGGAG TACCTGACCG CCGAGATCCT GGAGCTGGCT GGCAACGCGG 301 CCCGCGACAA CAAGAAGACT CGCATCATCC CGCGTCACCT CCAGCTGGCC ATCCGCAACG 361 ATGAGGAGCT CAACAAGCTT CTGGGCAAAG TCACCATCGC ACAGGGTGGC GTCCTGCCCA 421 ACATCCAGGC CGTGCTGCTG CCAAAGAAAA CTGAGAGCCA CCACAAGACT AAGTACCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAT CTACAGTCTC GTAGCTATGT TCAACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att