Construct: ORF TRCN0000474722

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013752.1_s317c1
Derived from:
ccsbBroadEn_04128
DNA Barcode:
TATCTAAGCAAAACTACTGACCCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG9 (79796)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474722
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_024740.2 100% 100%
2 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001077690.1 98.8% 98.8% 1170_1171ins21
3 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XM_005277723.5 98.1% 98% 1853_1887delinsGT
4 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352417.1 97% 96.9% 1170_1171ins21;1832_1866delinsGT
5 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XM_006718913.3 94.6% 93.5% (many diffs)
6 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XM_024448695.1 93.5% 92.4% (many diffs)
7 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XM_011542992.2 93.4% 92.8% (many diffs)
8 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XM_017018314.2 93.3% 93.3% 892_893ins123
9 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XM_017018313.2 92.3% 91.7% (many diffs)
10 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352418.1 92.2% 92.2% 892_893ins123;1047_1048ins21
11 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747980.1 83.7% (many diffs)
12 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747970.2 83.1% (many diffs)
13 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_947863.3 81.8% 1_88del;1819_2040del;2165_2266del
14 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_947864.2 81.5% 1_88del;1105_1106ins176;1767_1882del
15 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_947865.2 76.7% (many diffs)
16 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747967.2 74% 1_88del;1941_2502delinsGT
17 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747973.1 73.6% 1_99del;370_373delGTAG;1956_2513delinsGT
18 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747968.2 73.2% 1_88del;1258_1259ins21;1920_2480delinsGT
19 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001077691.2 72.3% 72.3% 0_1ins513
20 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352413.1 72.3% 72.3% 0_1ins513
21 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352414.2 72.3% 72.3% 0_1ins513
22 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747979.1 71.9% (many diffs)
23 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001077692.2 71.1% 71.1% 0_1ins513;657_658ins21
24 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352409.1 71.1% 71.1% 0_1ins513;657_658ins21
25 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352410.1 71.1% 71.1% 0_1ins513;657_658ins21
26 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352411.2 71.1% 71.1% 0_1ins513;657_658ins21
27 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352412.2 71.1% 71.1% 0_1ins513;657_658ins21
28 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352419.1 70.9% 70.9% 0_1ins513;1340_1374delinsGT
29 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352415.1 69.8% 69.7% 0_1ins513;657_658ins21;1319_1353delinsGT
30 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352416.1 69.8% 69.7% 0_1ins513;657_658ins21;1319_1353delinsGT
31 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747974.1 68.8% (many diffs)
32 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352422.2 67.1% 67.1% 0_1ins588;582_583ins21
33 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352421.2 66% 65% (many diffs)
34 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352420.2 65.4% 64.6% (many diffs)
35 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747971.1 64.7% (many diffs)
36 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747972.1 64.6% (many diffs)
37 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NM_001352423.2 64.5% 64.5% 0_1ins513;379_380ins123;534_535ins21
38 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747975.1 64% (many diffs)
39 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747976.1 63.9% (many diffs)
40 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747969.2 60.7% 1_88del;980_981ins123;1818_2723delinsGT
41 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... XR_001747977.1 56.1% (many diffs)
42 human 79796 ALG9 ALG9 alpha-1,2-mannosyltran... NR_147984.2 27.2% (many diffs)
43 mouse 102580 Alg9 asparagine-linked glycosyla... NM_133981.2 86.3% 92.4% (many diffs)
44 mouse 102580 Alg9 asparagine-linked glycosyla... XM_006509902.1 80.4% 85.9% (many diffs)
45 mouse 102580 Alg9 asparagine-linked glycosyla... XM_006509901.1 79.9% 79.7% (many diffs)
46 mouse 102580 Alg9 asparagine-linked glycosyla... XM_006509903.3 72.5% 69.6% (many diffs)
47 mouse 102580 Alg9 asparagine-linked glycosyla... XM_017313065.1 66.6% 63.3% (many diffs)
48 mouse 102580 Alg9 asparagine-linked glycosyla... NR_045601.1 54.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1920
ORF length:
1854
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tagtcgaggg gctcggcagc gcctgaaggg cagcggggcc agcagtgggg
121atacggcccc ggctgcggac aagctgcggg agctgctggg cagccgagag gcgggcggcg
181cggagcaccg gaccgagtta tctgggaaca aagcaggaca agtctgggca cctgaaggat
241ctactgcttt caagtgtctg ctttcagcaa ggttatgtgc tgctctcctg agcaacatct
301ctgactgtga tgaaacattc aactactggg agccaacaca ctacctcatc tatggggaag
361ggtttcagac ttgggaatat tccccagcat atgccattcg ctcctatgct tacctgttgc
421ttcatgcctg gccagctgca tttcatgcaa gaattctaca aactaataag attcttgtgt
481tttacttttt gcgatgtctt ctggcttttg tgagctgtat ttgtgaactt tacttttaca
541aggctgtgtg caagaagttt gggttgcacg tgagtcgaat gatgctagcc ttcttggttc
601tcagcactgg catgttttgc tcatcatcag cattccttcc tagtagcttc tgtatgtaca
661ctacgttgat agccatgact ggatggtata tggacaagac ttccattgct gtgctgggag
721tagcagctgg ggctatctta ggctggccat tcagtgcagc tcttggttta cccattgcct
781ttgatttgct ggtcatgaaa cacaggtgga agagtttctt tcattggtcg ctgatggccc
841tcatactatt tctggtgcct gtggtggtca ttgacagcta ctattatggg aagttggtga
901ttgcaccact caacattgtt ttgtataatg tctttactcc tcatggacct gatctttatg
961gtacagaacc ctggtatttc tatttaatta atggatttct gaatttcaat gtagcctttg
1021ctttggctct cctagtccta ccactgactt ctcttatgga atacctgctg cagagatttc
1081atgttcagaa tttaggccac ccgtattggc ttaccttggc tccaatgtat atttggttta
1141taattttctt catccagcct cacaaagagg agagatttct tttccctgtg tatccactta
1201tatgtctctg tggcgctgtg gctctctctg cacttcagca cagttttctg tacttccaga
1261aatgttacca ctttgtgttt caacgatatc gcctggagca ctatactgtg acatcgaatt
1321ggctggcatt aggaactgtc ttcctgtttg ggctcttgtc attttctcgc tctgtggcac
1381tgttcagagg atatcacggg ccccttgatt tgtatccaga attttaccga attgctacag
1441acccaaccat ccacactgtc ccagaaggca gacctgtgaa tgtctgtgtg ggaaaAGAGT
1501GGTATCGATT TCCCAGCAGC TTCCTTCTTC CTGACAATTG GCAGCTTCAG TTCATTCCAT
1561CAGAGTTCAG AGGTCAGTTA CCAAAACCTT TTGCAGAAGG ACCTCTGGCC ACCCGGATTG
1621TTCCTACTGA CATGAATGAC CAGAATCTAG AAGAGCCATC CAGATATATT GATATCAGTA
1681AATGCCATTA TTTAGTGGAT TTGGACACCA TGAGAGAAAC ACCCCGGGAG CCAAAATATT
1741CATCCAATAA AGAAGAATGG ATCAGCTTGG CCTATAGACC ATTCCTTGAT GCTTCTAGAT
1801CTTCAAAGCT GCTGCGGGCA TTCTATGTCC CCTTCCTGTC AGATCAGTAT ACAGTGTACG
1861TAAACTACAC CATCCTCAAA CCCCGGAAAG CAAAGCAAAT CAGGAAGAAA AGTGGAGGTT
1921ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1981GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2041TTTATATATC TTGTGGAAAG GACGATATCT AAGCAAAACT ACTGACCCTA CGCGTTAAGT
2101Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)