Construct: ORF TRCN0000474965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000385.1_s317c1
- Derived from:
- ccsbBroadEn_13307
- DNA Barcode:
- CAATAGGGCAGCGTGCACTATCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRUNE2 (158471)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474965
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_005251754.2 | 8.3% | 8.2% | (many diffs) |
| 2 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | NM_001308047.1 | 8.3% | 8.2% | (many diffs) |
| 3 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | NM_001308048.1 | 8.3% | 8.2% | (many diffs) |
| 4 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014353.2 | 8.3% | 8.2% | (many diffs) |
| 5 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014352.1 | 8.2% | 8.2% | (many diffs) |
| 6 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_011518328.2 | 8.2% | 8.2% | (many diffs) |
| 7 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014351.1 | 8.2% | 8.2% | (many diffs) |
| 8 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014350.1 | 8.2% | 8.2% | (many diffs) |
| 9 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_005251751.1 | 8.2% | 8.2% | (many diffs) |
| 10 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014349.1 | 8.2% | 8.2% | (many diffs) |
| 11 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014348.1 | 8.2% | 8.2% | (many diffs) |
| 12 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014347.2 | 8.2% | 8.2% | (many diffs) |
| 13 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_011518327.1 | 8.2% | 8.2% | (many diffs) |
| 14 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_011518326.2 | 8.2% | 8.2% | (many diffs) |
| 15 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014346.1 | 8.2% | 8.2% | (many diffs) |
| 16 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | NM_015225.3 | 8.2% | 8.2% | (many diffs) |
| 17 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_005251750.1 | 8.2% | 8.2% | (many diffs) |
| 18 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_011518323.2 | 8.2% | 8.2% | (many diffs) |
| 19 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_005251748.1 | 8.2% | 8.2% | (many diffs) |
| 20 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_005251746.1 | 8.2% | 8.2% | (many diffs) |
| 21 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_017014345.2 | 8.2% | 8.2% | (many diffs) |
| 22 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_005251745.1 | 8.2% | 8.2% | (many diffs) |
| 23 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_006716986.1 | 8.2% | 8.1% | (many diffs) |
| 24 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_006716984.2 | 8.2% | 8.1% | (many diffs) |
| 25 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_006716985.1 | 8.2% | 8.1% | (many diffs) |
| 26 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_006716983.1 | 8.2% | 8.1% | (many diffs) |
| 27 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XM_006716982.1 | 8.2% | 8.1% | (many diffs) |
| 28 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XR_001746211.2 | 5.9% | (many diffs) | |
| 29 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XR_001746210.2 | 5.9% | (many diffs) | |
| 30 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XR_001746209.2 | 5.9% | (many diffs) | |
| 31 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | XR_428517.3 | 5.9% | (many diffs) | |
| 32 | human | 158471 | PRUNE2 | prune homolog 2 with BCH do... | NR_131751.1 | 4.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaatttttg caacgcgcca aatctaaact gaatcgaagc aaacgcttgg 121 agaaggtcca tgtggttatt gggcctaaat cgtgtgactt ggattctctc atttctacct 181 tcacatatgc ttactttcta gacaaggtca gtccaccagg ggttctgtgt ttaccagtgc 241 tgaacatacc aagaactgaa ttcaactact tcaccgagac gaggtttatt ttagaagagc 301 taaatatttc cgaatcattc cacatattcc gggatgaaat taacctgcaT CAGCTAAATG 361 ATGAAGGGAA GTTATCGATA ACACTTGTTG GCAGCAGTGT GCTGGCGAGT GAAGACAAAA 421 CTTTAGAATC AGCAGTTGTC AAAGTCATTA ATCCGGTTGA GCAGAGCGAT GCCAACGTTG 481 AGTTCCGAGA GTCTTCCTCT TCTCTCGTGC TAAAGGAGAT TCTCCAAGAG GCTCCTGAGC 541 TCATCACCGA GCAACTGGCT CATCGCCTCA GAGGTAGCAT TCTTTTCAAG TGGATGACCA 601 TGGAATCAGA GAAGATCTCA GAGAAGCAGG AGGAAATTCT TTCTATCCTG GAAGAAAAAT 661 TTCCTAACTT GCCTCCAAGA GAGGACATCA TCAACGTCCT ACAGGAGACC CAGTTCAGTG 721 CTCAGGGTTT AAGTATTGAA CAGACAATGT TGAAAGATCT AAAGGAGCTG TCAGATGGAG 781 AAATAAAAGT GGCCATTAGT ACTGTGAGCA TGAACCTTGA GGTAAGGGTG GGAATGCTTT 841 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGACA ATAGGGCAGC GTGCACTATC GGACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt