Construct: ORF ccsbBroadEn_13307

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000385.1_s300c1, BRDN0000395585
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRUNE2 (158471)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251754.2 8.3% 8.2% (many diffs)
2 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308047.1 8.3% 8.2% (many diffs)
3 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308048.1 8.3% 8.2% (many diffs)
4 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014353.2 8.3% 8.2% (many diffs)
5 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014352.1 8.2% 8.2% (many diffs)
6 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518328.2 8.2% 8.2% (many diffs)
7 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014351.1 8.2% 8.2% (many diffs)
8 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014350.1 8.2% 8.2% (many diffs)
9 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251751.1 8.2% 8.2% (many diffs)
10 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014349.1 8.2% 8.2% (many diffs)
11 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014348.1 8.2% 8.2% (many diffs)
12 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014347.2 8.2% 8.2% (many diffs)
13 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518327.1 8.2% 8.2% (many diffs)
14 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518326.2 8.2% 8.2% (many diffs)
15 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014346.1 8.2% 8.2% (many diffs)
16 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_015225.3 8.2% 8.2% (many diffs)
17 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251750.1 8.2% 8.2% (many diffs)
18 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518323.2 8.2% 8.2% (many diffs)
19 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251748.1 8.2% 8.2% (many diffs)
20 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251746.1 8.2% 8.2% (many diffs)
21 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014345.2 8.2% 8.2% (many diffs)
22 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251745.1 8.2% 8.2% (many diffs)
23 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716986.1 8.2% 8.1% (many diffs)
24 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716984.2 8.2% 8.1% (many diffs)
25 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716985.1 8.2% 8.1% (many diffs)
26 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716983.1 8.2% 8.1% (many diffs)
27 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716982.1 8.2% 8.1% (many diffs)
28 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746211.2 5.9% (many diffs)
29 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746210.2 5.9% (many diffs)
30 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746209.2 5.9% (many diffs)
31 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_428517.3 5.9% (many diffs)
32 human 158471 PRUNE2 prune homolog 2 with BCH do... NR_131751.1 4.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
843
ORF length:
777
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA AGAATTTTTG CAACGCGCCA AATCTAAACT GAATCGAAGC AAACGCTTGG
121AGAAGGTCCA TGTGGTTATT GGGCCTAAAT CGTGTGACTT GGATTCTCTC ATTTCTACCT
181TCACATATGC TTACTTTCTA GACAAGGTCA GTCCACCAGG GGTTCTGTGT TTACCAGTGC
241TGAACATACC AAGAACTGAA TTCAACTACT TCACCGAGAC GAGGTTTATT TTAGAAGAGC
301TAAATATTTC CGAATCATTC CACATATTCC GGGATGAAAT TAACCTGCAT CAGCTAAATG
361ATGAAGGGAA GTTATCGATA ACACTTGTTG GCAGCAGTGT GCTGGCGAGT GAAGACAAAA
421CTTTAGAATC AGCAGTTGTC AAAGTCATTA ATCCGGTTGA GCAGAGCGAT GCCAACGTTG
481AGTTCCGAGA GTCTTCCTCT TCTCTCGTGC TAAAGGAGAT TCTCCAAGAG GCTCCTGAGC
541TCATCACCGA GCAACTGGCT CATCGCCTCA GAGGTAGCAT TCTTTTCAAG TGGATGACCA
601TGGAATCAGA GAAGATCTCA GAGAAGCAGG AGGAAATTCT TTCTATCCTG GAAGAAAAAT
661TTCCTAACTT GCCTCCAAGA GAGGACATCA TCAACGTCCT ACAGGAGACC CAGTTCAGTG
721CTCAGGGTTT AAGTATTGAA CAGACAATGT TGAAAGATCT AAAGGAGCTG TCAGATGGAG
781AAATAAAAGT GGCCATTAGT ACTGTGAGCA TGAACCTTGA GGTAAGGGTG GGAATGCTTT
841TTTACCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
901gcaacgaac

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