Transcript: Human XM_017014348.1

PREDICTED: Homo sapiens prune homolog 2 with BCH domain (PRUNE2), transcript variant X19, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRUNE2 (158471)
Length:
12682
CDS:
258..9488

Additional Resources:

NCBI RefSeq record:
XM_017014348.1
NBCI Gene record:
PRUNE2 (158471)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017014348.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000156077 CCCAATGGATTGCATCCACAT pLKO.1 9371 CDS 100% 4.050 5.670 N PRUNE2 n/a
2 TRCN0000154470 GCGCATTGACATGAAGGTCAT pLKO.1 8936 CDS 100% 4.050 5.670 N PRUNE2 n/a
3 TRCN0000122589 CGGGATGAAATTAACCTGCAT pLKO.1 522 CDS 100% 2.640 3.696 N PRUNE2 n/a
4 TRCN0000121740 CTGCATCAGCTAAATGATGAA pLKO.1 537 CDS 100% 0.495 0.693 N PRUNE2 n/a
5 TRCN0000144868 GCTCAGGGTTTAAGTATTGAA pLKO.1 912 CDS 100% 5.625 4.500 N PRUNE2 n/a
6 TRCN0000156045 CGGTGACAACAGTACAGGTTT pLKO.1 6845 CDS 100% 4.950 3.960 N PRUNE2 n/a
7 TRCN0000219933 TCTAAACTGAATCGAAGCAAA pLKO.1 285 CDS 100% 4.950 3.960 N PRUNE2 n/a
8 TRCN0000153497 CGTTCCCAAAGAACGAAGATA pLKO.1 5677 CDS 100% 0.563 0.450 N PRUNE2 n/a
9 TRCN0000145451 GCTGTCAGATGGAGAAATAAA pLKO.1 959 CDS 100% 15.000 10.500 N PRUNE2 n/a
10 TRCN0000139419 CTTCACCGAGACGAGGTTTAT pLKO.1 461 CDS 100% 13.200 9.240 N PRUNE2 n/a
11 TRCN0000152498 GCTTGAGAATCCAGGGTATTT pLKO.1 4166 CDS 100% 13.200 9.240 N PRUNE2 n/a
12 TRCN0000151765 CCTTCTTAGAAGCACTTCTTT pLKO.1 10999 3UTR 100% 5.625 3.938 N PRUNE2 n/a
13 TRCN0000155484 GCCAACAAAGATTCTGGCCAA pLKO.1 8826 CDS 100% 2.160 1.512 N PRUNE2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017014348.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13307 pDONR223 100% 8.2% 8.2% None (many diffs) n/a
2 ccsbBroad304_13307 pLX_304 0% 8.2% 8.2% V5 (many diffs) n/a
3 TRCN0000474965 CAATAGGGCAGCGTGCACTATCGG pLX_317 63.2% 8.2% 8.2% V5 (many diffs) n/a
4 ccsbBroadEn_13308 pDONR223 100% 6% 6% None (many diffs) n/a
5 ccsbBroad304_13308 pLX_304 0% 6% 6% V5 (many diffs) n/a
6 TRCN0000471170 ATCTCCTTCTAATACTGGGGTTTC pLX_317 77.5% 6% 6% V5 (many diffs) n/a
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