Construct: ORF TRCN0000475450
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017962.1_s317c1
- Derived from:
- ccsbBroadEn_08799
- DNA Barcode:
- ACTGCCGATATAGAACTCCATGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HPSE2 (60495)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475450
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60495 | HPSE2 | heparanase 2 (inactive) | NM_021828.4 | 99.8% | 99.6% | 1540G>A;1736A>T |
| 2 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_017016495.1 | 91.7% | 90.8% | (many diffs) |
| 3 | human | 60495 | HPSE2 | heparanase 2 (inactive) | NM_001166246.1 | 91.7% | 90.7% | (many diffs) |
| 4 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_011540029.1 | 91.6% | 91.5% | 1463_1464ins147;1589A>T |
| 5 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_011540030.1 | 90.7% | 90.5% | 447_448ins162;1378G>A;1574A>T |
| 6 | human | 60495 | HPSE2 | heparanase 2 (inactive) | NM_001166244.1 | 90% | 89.8% | 609_610ins174;1366G>A;1562A>T |
| 7 | human | 60495 | HPSE2 | heparanase 2 (inactive) | NM_001166245.1 | 80.9% | 80.7% | 445_446ins336;1204G>A;1400A>T |
| 8 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_017016496.2 | 71% | 59.3% | (many diffs) |
| 9 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_006717937.2 | 70.8% | 70.6% | 0_1ins516;1024G>A;1220A>T |
| 10 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_011540031.2 | 70.8% | 70.6% | 0_1ins516;1024G>A;1220A>T |
| 11 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_017016497.1 | 70.8% | 70.6% | 0_1ins516;1024G>A;1220A>T |
| 12 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_024448119.1 | 70.8% | 70.6% | 0_1ins516;1024G>A;1220A>T |
| 13 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_017016498.1 | 54.6% | 54.3% | 0_1ins804;736G>A;932A>T |
| 14 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XM_024448120.1 | 54.6% | 54.3% | 0_1ins804;736G>A;932A>T |
| 15 | human | 60495 | HPSE2 | heparanase 2 (inactive) | XR_001747170.1 | 40.7% | (many diffs) | |
| 16 | mouse | 545291 | Hpse2 | heparanase 2 | NM_001081257.2 | 93% | 97.1% | (many diffs) |
| 17 | mouse | 545291 | Hpse2 | heparanase 2 | XM_006527214.3 | 83.3% | 87.3% | (many diffs) |
| 18 | mouse | 545291 | Hpse2 | heparanase 2 | XM_011247294.2 | 71.1% | 72% | (many diffs) |
| 19 | mouse | 545291 | Hpse2 | heparanase 2 | XM_011247295.1 | 65.7% | 69.7% | (many diffs) |
| 20 | mouse | 545291 | Hpse2 | heparanase 2 | XM_011247296.2 | 53% | 53.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1845
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagggtgctt tgtgccttcc ctgaagccat gccctccagc aactcccgcc 121 cccccgcgtg cctagccccg ggggctctct acttggctct gttgctccat ctctcccttt 181 cctcccaggc tggagacagg agacccttgc ctgtagacag agctgcaggt ttgaaggaaa 241 agaccctgat tctacttgat gtgagcacca agaacccagt caggacagtc aatgagaact 301 tcctctctct gcagctggat ccgtccatca ttcatgatgg ctggctcgat ttcctaagct 361 ccaagcgctt ggtgaccctg gcccggggac tttcgcccgc ctttctgcgc ttcgggggca 421 aaaggaccga cttcctgcag ttccagaacc tgaggaaccc ggcgaaaagc cgcgggggcc 481 cgggcccgga ttactatctc aaaaactatg aggatgacat tgttcgaagt gatgttgcct 541 tagataaaca gaaaggctgc aagattgccc agcaccctga tgttatgctg gagctccaaa 601 gggagaaggc agctcagatg catctggttc ttctaaagga gcaattctcc aatacttaca 661 gtaatctcat attaacagcc aggtctctag acaaacttta taactttgct gattgctctg 721 gactccacct gatatttgct ctaaatgcac tgcgtcgtaa tcccaataac tcctggaaca 781 gttctagtgc cctgagtctg ttgaagtaca gcgccagcaa aaagtacaac atttcttggg 841 aactgggtaa tgagccaaat aactatcgga ccatgcatgg ccgggcagta aatggcagcc 901 agttgggaaa ggattacatc cagctgaaga gcctgttgca gcccatccgg atttattcca 961 gagccagctt atatggccct aatattgggc ggccgaggaa gaatgtcatc gccctcctag 1021 atggattcat gaaggtggca ggaagtacag tagatgcagt tacctggcaa cattgctaca 1081 ttgatggccg ggtggtcaag gtgatggact tcctgaaaac tcgcctgtta gacacactct 1141 ctgaccagat taggaaaatt cagaaagtgg ttaatacata cactccagga aagaagattt 1201 ggcttgaagg tgtggtgacc acctcagctg gaggcacaaa caatctatcc gattcctatg 1261 ctgcaggatt cttatggttg aacactttag gaatgctggc caatcagggc attgatgtcg 1321 tgatacggca ctcatttttt gaccatggat acaatcacct cgtggaccag aattttaacc 1381 cattaccaga ctactggctc tctctcctct acaagcgcct gatcggcccc aaagtcttGG 1441 CTGTGCATGT GGCTGGGCTC CAGCGGAAGC CACGGCCTGG CCGAGTGATC CGGGACAAAC 1501 TAAGGATTTA TGCTCACTGC ACAAACCACC ACAACCACAA CTACGTTCGT GGGTCCATTA 1561 CACTTTTTAT CATCAACTTG CATCGATCAA GAAAGAAAAT CAAGCTGACT GGGACTCTCA 1621 GAGACAAGCT GGTTCACCAG TACCTGCTGC AGCCCTATGG GCAGGAGGGC CTAAAGTCCA 1681 AGTCAGTGCA ACTGAATGGC CAGCCCTTAG TGATGGTGGA CGACGGGACC CTCCCAGAAT 1741 TGAAGCCCCG CCCCCTTCGG GCCGGCCGGA CATTGGTCAT CCCTCCAGTC ACCATGGGCT 1801 TTTTTGTGGT CAAGAATGTC AATGCTTTGG CCTGCCGCTA CCGATTGCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA ACTGCCGATA TAGAACTCCA TGTAACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt