Construct: ORF TRCN0000475456
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017804.1_s317c1
- Derived from:
- ccsbBroadEn_07865
- DNA Barcode:
- GTACCCCTTTTGGGTAAATGACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBR2 (23304)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475456
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | NM_001184801.2 | 99.9% | 100% | 717T>C |
| 2 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | XM_017010597.1 | 33.4% | 32% | (many diffs) |
| 3 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | XR_001743285.1 | 28.5% | (many diffs) | |
| 4 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | XM_017010596.1 | 24.5% | 24.2% | (many diffs) |
| 5 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | NM_001363705.1 | 24.4% | 24.2% | (many diffs) |
| 6 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | NM_015255.2 | 23.9% | 23.4% | (many diffs) |
| 7 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | XM_017010595.1 | 23.6% | 22.7% | (many diffs) |
| 8 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | XM_011514438.2 | 23.6% | 22.6% | (many diffs) |
| 9 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | XM_017010594.1 | 23.1% | 21.9% | (many diffs) |
| 10 | human | 23304 | UBR2 | ubiquitin protein ligase E3... | XR_001743284.2 | 15.6% | (many diffs) | |
| 11 | mouse | 224826 | Ubr2 | ubiquitin protein ligase E3... | XR_001782069.1 | 40.2% | (many diffs) | |
| 12 | mouse | 224826 | Ubr2 | ubiquitin protein ligase E3... | NM_001177374.1 | 21.7% | 22.6% | (many diffs) |
| 13 | mouse | 224826 | Ubr2 | ubiquitin protein ligase E3... | NM_146078.3 | 20.8% | 21.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1383
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcggagcta gagccagagg tgcaggccat cgaccggagc ttgctggaat 121 gttcggccga ggagattgcg gggaaatggc tgcaagcaac tgacctcact agagaagtgt 181 accagcattt agcccactat gtacccaaaa tctactgcag gggtcccaac ccttttccac 241 agaaagaaga catgctggca cagcatgttt tgttgggacc aatggaatgg tacctttgtg 301 gtgaagatcc tgcatttgga tttccaaaac ttgagcaagc aaacaaacct tctcatcttt 361 gtggtcgtgt ttttaaagta ggagagccta catattcttg cagagactgt gcagttgatc 421 caacttgtgt tttgtgcatg gagtgctttt tgggaagtat tcacagagat catcgatata 481 ggatgacaac atcaggaggt ggaggtttct gtgactgtgg tgatactgaa gcctggaaag 541 agggtcctta ctgtcaaaaa catgaactta acacctctga aattgaggaa gaagaggatc 601 ctcttgttca tttatcagaa gatgtgatag caagaactta taacattttt gctattacgt 661 ttcggtatgc agtagaaata ttaacctggg aaaaagaaag tgaattgcca gcagatttag 721 agatggtaga gaagagtgac acctactatt gcatgctgtt taatgatgag gttcacacct 781 acgaacaagt tatttatacT CTTCAGAAAG CTGTTAACTG TACACAAAAA GAAGCTATTG 841 GTTTTGCAAC TACAGTAGAT CGAGATGGGC GTAGGTCTGT TCGATATGGA GATTTTCAGT 901 ATTGTGAGCA AGCAAAATCA GTAATTGTGA GAAATACCAG TAGACAGACA AAGCCACTCA 961 AAGTTCAAGT TATGCATTCG TCTATTGTCG CACATCAGAA TTTTGGTTTG AAACTTTTGT 1021 CTTGGCTGGG AAGTATTATT GGATATTCAG ATGGCCTTCG CCGGATTTTA TGTCAAGTTG 1081 GTTTACAAGA AGGGCCAGAT GGTGAAAACT CTTCTCTAGT GGACAGACTG ATGCTTAGTG 1141 ATTCCAAATT ATGGAAAGGT GCTAGGAGTG TATATCATCA GTTGTTCATG AGCAGTCTGC 1201 TTATGGATTT GAAATACAAG AAACTATTTG CTGTTCGATT TGCAAAAAAC TATGAGCGTT 1261 TGCAGAGTGA TTATGTGACA GATGACCACG ACAGAGAGTT TTCAGTCGCA GACCTCTCGG 1321 TTCAGATATT CACGGTTCCT TCACTTTTCT CTATCTCTGC TGGGCGCAGT GGCTCACCTC 1381 TGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGAGT ACCCCTTTTG GGTAAATGAC CAACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt