Construct: ORF TRCN0000477195
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010953.1_s317c1
- Derived from:
- ccsbBroadEn_05418
- DNA Barcode:
- AGCCGCGAGTGTTGAGATGACAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIN9 (286826)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477195
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_173083.3 | 100% | 100% | |
| 2 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_001366238.1 | 99.8% | 99.8% | 109_110insAAG |
| 3 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_001366245.2 | 97.1% | 97.1% | 0_1ins48 |
| 4 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_001366237.1 | 95.1% | 95.1% | 1084_1085ins81 |
| 5 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_001366239.1 | 93.9% | 93.9% | 984_985ins102 |
| 6 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_001270409.1 | 93.7% | 93.7% | 206_207ins105 |
| 7 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | XM_005273102.3 | 88.3% | 87.4% | (many diffs) |
| 8 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_001270410.2 | 87.8% | 87.8% | 0_1ins204 |
| 9 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_001366241.1 | 87.8% | 87.8% | 0_1ins204 |
| 10 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | XM_006711767.4 | 87.8% | 87.8% | 0_1ins204 |
| 11 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | XM_024446565.1 | 82.8% | 81.9% | (many diffs) |
| 12 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | NM_001366240.1 | 80.4% | 80.4% | 0_1ins327 |
| 13 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | XM_017001084.1 | 77.2% | 76.2% | (many diffs) |
| 14 | human | 286826 | LIN9 | lin-9 DREAM MuvB core compl... | XM_011544173.2 | 56.2% | 56.2% | 1_402del;1569_1570ins507 |
| 15 | mouse | 72568 | Lin9 | lin-9 homolog (C. elegans) | NM_001103182.2 | 90.2% | 97.1% | (many diffs) |
| 16 | mouse | 72568 | Lin9 | lin-9 homolog (C. elegans) | XM_006496987.1 | 78.6% | 85.4% | (many diffs) |
| 17 | mouse | 72568 | Lin9 | lin-9 homolog (C. elegans) | XM_006496988.3 | 78.6% | 85.4% | (many diffs) |
| 18 | mouse | 72568 | Lin9 | lin-9 homolog (C. elegans) | NR_120498.1 | 47.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1743
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaccgcggc gggcagcctt tgaaaaagcg gcgcggctcg ttcaagatgg 121 cggagctcga ccagttgcct gacgagagct cttcagcaaa agcccttgtc agtttaaaag 181 aaggaagctt atctaacacg tggaatgaaa agtacagttc tttacagaaa acacctgttt 241 ggaaaggcag gaatacaagc tctgctgtgg aaatgccttt cagaaattca aaacgaagtc 301 gacttttttc tgatgaagat gataggcaaa taaatacaag gtcacctaaa agaaaccaga 361 gggttgcaat ggttccacag aaatttacag caacaatgtc aacaccagat aagaaagctt 421 cacagaagat tggttttcga ttacgtaatc tgctcaagct tcctaaagca cataaatggt 481 gtatatacga gtggttctat tcaaatatag ataaaccact ttttgaaggt gataatgact 541 tctgtgtatg tctaaaggaa tcttttccta atttgaaaac aagaaagtta acaagagtag 601 aatggggaaa aattcggcgg cttatgggaa aaccacggag atgttcttct gcattttttg 661 aggaagagag atcagcatta aaacagaaac ggcagaaaat aaggctctta caacaaagga 721 aagttgcaga tgtttcacaa ttcaaagatc tcccagatga aattcctttg cctctggtta 781 ttggaacgaa agttacagca cgattacgtg gtgttcatga tggtttgttc actggacaaa 841 tagatgctgt ggatactctt aatgctactt atagagtaac ttttgatagg acagggcttg 901 gaacccatac catccctgac tatgaagttc tcagtaatga acctcatgag acaatgccaa 961 ttgctgcctt tggacaaaaa cagcggcctt ctcgattttt tatgacccca ccacggttac 1021 attatactcc tcctctccag tcaccaatta tagataatga tcctttatta ggacagtcgc 1081 cgtggagaag taaaatttct ggctctgaca ctgaaacatt aggtggtttt ccagtagaat 1141 ttcttatcca agtgaccaga ttatcaaaaa ttctcatgat taaaaaggaa catatcaaga 1201 aattaaggga aatgaacaca gaagcagaaa aattgaaatc atattccatg cccatcagca 1261 ttgaatttca gcggagatat gcaacaattg ttctggagct tgaacagctg aacaaggacc 1321 taaacaaagt tttgcataaa gttcaacagt attgctatga gcttgctcca gaccaggggc 1381 tccagcctgc agatcagcca acagatatga gacgcaggtg tgaggaagaa gcacaggaaa 1441 ttgttcggca tgcaaattcc tcaacaggac agcccTGCGT TGAAAATGAA AATCTGACAG 1501 ACTTAATTTC CAGGCTTACA GCTATTTTGT TACAAATTAA GTGTCTAGCA GAAGGAGGAG 1561 ACCTGAATTC CTTTGAATTC AAATCACTTA CAGACTCATT AAATGATATC AAGAGTACAA 1621 TAGACGCTTC TAATATCAGT TGCTTTCAGA ATAATGTAGA AATCCATGTT GCACATATTC 1681 AGAGTGGCCT GAGCCAGATG GGAAACTTAC ATGCCTTTGC AGCAAATAAC ACCAACAGAG 1741 ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1801 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1861 GGCTTTATAT ATCTTGTGGA AAGGACGAAG CCGCGAGTGT TGAGATGACA ATACGCGTTA 1921 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt