Construct: ORF TRCN0000477516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007580.1_s317c1
- Derived from:
- ccsbBroadEn_12261
- DNA Barcode:
- CGCTTCGGCAGCTTCAACGGCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF7IP (55729)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477516
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55729 | ATF7IP | activating transcription fa... | NM_001286515.2 | 99.9% | 99.9% | 1586A>G |
| 2 | human | 55729 | ATF7IP | activating transcription fa... | NM_001286514.2 | 86.7% | 86.1% | (many diffs) |
| 3 | human | 55729 | ATF7IP | activating transcription fa... | XM_017019639.1 | 86.7% | 86.1% | (many diffs) |
| 4 | human | 55729 | ATF7IP | activating transcription fa... | NM_018179.4 | 86.6% | 86% | (many diffs) |
| 5 | human | 55729 | ATF7IP | activating transcription fa... | XM_006719108.3 | 86.6% | 86% | (many diffs) |
| 6 | human | 55729 | ATF7IP | activating transcription fa... | XM_006719109.3 | 86.6% | 86% | (many diffs) |
| 7 | human | 55729 | ATF7IP | activating transcription fa... | XM_011520754.3 | 86.6% | 86% | (many diffs) |
| 8 | human | 55729 | ATF7IP | activating transcription fa... | XM_011520755.2 | 86.6% | 86% | (many diffs) |
| 9 | human | 55729 | ATF7IP | activating transcription fa... | XM_017019638.1 | 86.6% | 86% | (many diffs) |
| 10 | human | 55729 | ATF7IP | activating transcription fa... | XM_005253424.4 | 86.2% | 85.5% | (many diffs) |
| 11 | human | 55729 | ATF7IP | activating transcription fa... | NM_181352.2 | 86.1% | 85.5% | (many diffs) |
| 12 | human | 55729 | ATF7IP | activating transcription fa... | XM_011520756.3 | 85.9% | 85.7% | (many diffs) |
| 13 | human | 55729 | ATF7IP | activating transcription fa... | XR_001748807.2 | 84.1% | (many diffs) | |
| 14 | human | 55729 | ATF7IP | activating transcription fa... | XR_001748806.2 | 84.1% | (many diffs) | |
| 15 | human | 55729 | ATF7IP | activating transcription fa... | XR_001748809.2 | 81.1% | (many diffs) | |
| 16 | human | 55729 | ATF7IP | activating transcription fa... | XR_001748808.2 | 36.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3384
- ORF length:
- 3315
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacagttta gaagaacctc agaaaaaagt ctttaaggct cgaaaaacga 121 tgagagtgag tgatcgtcag caacttgaag cagtgtacaa ggtcaaagaa gaactgttga 181 aaactgatgt caagctgtta aatggcaacc atgaaaatgg agatttggac ccaacctcac 241 ctttggaaaa catggattac attaaagaca aggaagaggt gaatggcatt gaagagattt 301 gttttgatcc tgaaggaagt aaagcagaat ggaaggaaac accctgtatc ctaagtgtta 361 atgtaaaaaa caagcaggat gatgatttaa attgtgaacc tttgtctccc cataatataa 421 ctccagaacc agtctctaaa ctgcctgctg aaccagtttc tggtgatcca gcccctggtg 481 atctggatgc cggagatcca gcctccggag tactggcctc tggtgattcc acctctggtg 541 atcccacctc tagcgagccc tcctctagtg atgctgcctc tggtgatgca acctctggtg 601 atgccccttc tggtgatgtg tcccctggtg atgccacctc tggtgatgcc actgctgatg 661 atctctcctc tggtgatccc acctctagtg atcccatccc aggtgaaccg gtccctgttg 721 aacccatttc tggtgattgt gccgctgatg atatagcctc tagtgaaata acttctgttg 781 atctggcttc tggagcacca gcttccactg atccagcctc tgatgatctg gcctctggtg 841 atctatcctc tagtgaactg gcctctgatg atctggccac tggtgaactg gcctctgatg 901 agctgacttc tgaatcaacc tttgatcgta cctttgaacc aaagtctgta ccagtttgtg 961 aaccagttcc tgaaattgac aatatagaac caagtagcaa taaagatgat gattttcttg 1021 aaaaaaatgg agctgatgaa aaattagagc aaattcagag taaagactca ttggatgaga 1081 aaaataaagc tgataataat attgatgcta atgaagaaac tctagaaaca gatgatacaa 1141 ctatttgttc agatcgacct cctgaaaatg aaaagaaggt agaggaagat attatcacag 1201 agcttgctct tggagaagat gctatatcta gcagtatgga aattgaccaa ggtgaaaaga 1261 atgaagatga aacttctgca gatcttgtag aaacgattaa tgaaaatgtt attgaagata 1321 acaaaagtga gaatatctta gaaaatacag actctatgga gacagatgaa atcattccta 1381 ttttggaaaa gcttgcacct tctgaggatg aacttacttg cttttctaaa acatctctcc 1441 ttccaatcga tgagacaaat ccagatttgg aagagaaaat ggaaagttct tttggttcac 1501 catctaaaca agaaagtagt gagagtttgc caaaagaagc ctttctggtc ctctctgatg 1561 aagaggatat ttcgggtgaa aaagatgagt ctgaagttat atcgcaaaat gaaacgtgct 1621 ctccagaagt agaaagtaat gaaaaggaca acagacctga ggaagaagag caagtaatac 1681 atgaagatga tgaaagacct tctgagaaaa atgaattttc tagacgaaaa cgttctaaat 1741 cagaagacat ggacaatgta cagtctaaac gtcgtcgata tatggaagaa gaatatgagg 1801 cagaatttca agtaaagatt acagccaaag gagacattaa ccagaaactt caaaaggtta 1861 tacagtggtt gctggaagaa aaattgtgtg cgctgcagtg tgctgtattt gataagactt 1921 tggcagaatt gaaaacacga gtggaaaaga ttgaatgtaa caagaggcat aaaacagttc 1981 tcactgaact acaggccaag atagccaggt taaccaaacg ctttgaagca gccaaagaag 2041 atcttaagaa aagacatgaa catccaccca acccaccagt atcaccagga aaaactgtaa 2101 atgatgtcaa cagcaataat aacatgtctt acagaaatgc aggcacagtg agacagatgc 2161 tggagtccaa aagaaatgta agcgagagtg caccaccatc ctttcaaact cctgtgaata 2221 cagtatcttc aaccaatctt gtcactcctc cagcagttgt cagtagtcaa cctaaattgc 2281 agactccagt gacttcgggt tccctcacag caacgtcagt tcttcctgca cccaatacag 2341 ctactgtagt tgctactact caggtgccta gtggaaatcc ccagcctaca atctctttac 2401 agcctttgcc agtgattttg catgtacctg ttgcagtatc ctcccagcct cagcttctac 2461 agagccatcc agggactttg gtgactaatc aaccatctgg caatgttgaa ttcatttctg 2521 tgcaaagccc acctacagtg agtggtctta ccaaaaatcc agtatccttg ccatccttgc 2581 caaatcccac taaaccaaac aacgttcctt ctgtgcccag tcctagtatt caaaggaacc 2641 ctactgccag tgctgcacca ttgggaacaa cacttgctgt gcaggctgtt ccaacagcac 2701 actctattgt acaagccaca aggacttctt tacccacagt gggcccatca ggactctata 2761 gtccatcaac taatcgaggt cctatacaga tgaaaattcc aatttctgca tttagtactt 2821 cgtctgctgc agaacagaac agcaatacca ccccaagaat tgaaaaccag acaaacaaaa 2881 caatagatgc TTCTGTCAGT AAGAAAGCAG CTGATAGCAC ATCACAGTGT GGAAAAGCCA 2941 CTGGCAGTGA TTCAAGTGGT GTCATTGATC TCACAATGGA TGATGAAGAG AGTGGAGCTT 3001 CACAAGACCC CAAAAAACTA AATCACACTC CTGTATCAAC CATGAGTTCT TCTCAGCCTG 3061 TGTCACGACC ATTGCAACCC ATACAACCAG CACCGCCTCT TCAACCATCT GGGGTGCCAA 3121 CAAGTGGACC ATCTCAGACC ACCATACACT TACTACCTAC AGCTCCAACT ACCGTGAATG 3181 TAACACATCG TCCAGTAACT CAGGTGACCA CAAGACTCCC TGTACCAAGA GCTCCTGCAA 3241 ACCACCAGGT GGTTTATACA ACTCTTCCTG CACCACCAGC TCAGGCTCCC TTGCGAGGAA 3301 CTGTTATGCA GGCTCCTGCT GTTCGGCAGG TCAATCCCCA AAATAGTAAG AGATTTTTCT 3361 TGTATATGGC CCCAAGATAC ATGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3421 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3481 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GCTTCGGCAG 3541 CTTCAACGGC GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3601 att