Construct: ORF TRCN0000478286
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012669.1_s317c1
- Derived from:
- ccsbBroadEn_03650
- DNA Barcode:
- ACAGCCCAAGTATGTTGCTGTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AJ (55766)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478286
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55766 | H2AJ | H2A.J histone | NM_177925.3 | 100% | 100% | |
| 2 | human | 8332 | H2AC16 | H2A clustered histone 16 | NM_003511.2 | 84.8% | 95.3% | (many diffs) |
| 3 | human | 8330 | H2AC15 | H2A clustered histone 15 | NM_003510.2 | 83.8% | 95.3% | (many diffs) |
| 4 | human | 92815 | H2AW | H2A.W histone | NM_033445.2 | 83.5% | 94.6% | (many diffs) |
| 5 | human | 8329 | H2AC13 | H2A clustered histone 13 | NM_003509.2 | 83.3% | 95.3% | (many diffs) |
| 6 | human | 8334 | H2AC6 | H2A clustered histone 6 | NM_003512.3 | 83% | 95.3% | (many diffs) |
| 7 | human | 3013 | H2AC7 | H2A clustered histone 7 | NM_021065.3 | 83% | 94.6% | (many diffs) |
| 8 | human | 723790 | H2AC19 | H2A clustered histone 19 | NM_001040874.1 | 82.8% | 95.3% | (many diffs) |
| 9 | human | 8337 | H2AC18 | H2A clustered histone 18 | NM_003516.2 | 82.8% | 95.3% | (many diffs) |
| 10 | human | 8338 | H2AC20 | H2A clustered histone 20 | NM_003517.2 | 82.6% | 96.8% | (many diffs) |
| 11 | human | 8969 | H2AC11 | H2A clustered histone 11 | NM_021064.4 | 82.3% | 95.3% | (many diffs) |
| 12 | human | 8336 | H2AC17 | H2A clustered histone 17 | NM_003514.2 | 82% | 95.3% | (many diffs) |
| 13 | human | 8331 | H2AC14 | H2A clustered histone 14 | NM_021066.2 | 82% | 95.3% | (many diffs) |
| 14 | human | 85235 | H2AC12 | H2A clustered histone 12 | NM_080596.2 | 81.9% | 95.3% | (many diffs) |
| 15 | human | 3012 | H2AC8 | H2A clustered histone 8 | NM_021052.2 | 80% | 93.8% | (many diffs) |
| 16 | human | 317772 | H2AC21 | H2A clustered histone 21 | NM_175065.2 | 79.4% | 94.6% | (many diffs) |
| 17 | human | 3014 | H2AX | H2A.X variant histone | NM_002105.2 | 75.6% | 83.2% | (many diffs) |
| 18 | human | 55766 | H2AJ | H2A.J histone | NR_027716.2 | 11.4% | 1_135del;523_3385del | |
| 19 | mouse | 232440 | H2afj | H2A histone family, member J | NM_177688.4 | 87% | 98.4% | (many diffs) |
| 20 | mouse | 319176 | Hist2h2ac | histone cluster 2, H2ac | NM_175662.2 | 86.3% | 96.8% | (many diffs) |
| 21 | mouse | 15267 | Hist2h2aa1 | histone cluster 2, H2aa1 | NM_013549.2 | 85.8% | 95.3% | (many diffs) |
| 22 | mouse | 319192 | Hist2h2aa2 | histone cluster 2, H2aa2 | NM_178212.3 | 85.8% | 95.3% | (many diffs) |
| 23 | mouse | 319167 | Hist1h2ag | histone cluster 1, H2ag | NM_178186.3 | 85.8% | 93.8% | (many diffs) |
| 24 | mouse | 319165 | Hist1h2ad | histone cluster 1, H2ad | NM_178188.4 | 85.8% | 93.8% | (many diffs) |
| 25 | mouse | 319170 | Hist1h2an | histone cluster 1, H2an | NM_178184.2 | 85.6% | 93.8% | (many diffs) |
| 26 | mouse | 319166 | Hist1h2ae | histone cluster 1, H2ae | NM_178187.4 | 85.6% | 93.8% | (many diffs) |
| 27 | mouse | 319173 | Hist1h2af | histone cluster 1, H2af | NM_175661.2 | 85.4% | 93.8% | (many diffs) |
| 28 | mouse | 665433 | Hist1h2ao | histone cluster 1, H2ao | NM_001177544.2 | 85.3% | 93.8% | (many diffs) |
| 29 | mouse | 319191 | Hist1h2ai | histone cluster 1, H2ai | NM_178182.2 | 85.3% | 93.8% | (many diffs) |
| 30 | mouse | 319171 | Hist1h2ap | histone cluster 1, H2ap | NM_178185.2 | 85.3% | 93.8% | (many diffs) |
| 31 | mouse | 319164 | Hist1h2ac | histone cluster 1, H2ac | NM_178189.5 | 85.3% | 93.8% | (many diffs) |
| 32 | mouse | 319172 | Hist1h2ab | histone cluster 1, H2ab | NM_175660.3 | 84.8% | 93.8% | (many diffs) |
| 33 | mouse | 319168 | Hist1h2ah | histone cluster 1, H2ah | NM_175659.2 | 84.8% | 93.7% | (many diffs) |
| 34 | mouse | 319169 | Hist1h2ak | histone cluster 1, H2ak | NM_178183.2 | 84.3% | 93% | (many diffs) |
| 35 | mouse | 319162 | Hist3h2a | histone cluster 3, H2a | NM_178218.4 | 81% | 94.6% | (many diffs) |
| 36 | mouse | 621893 | Hist2h2ab | histone cluster 2, H2ab | NM_178213.4 | 80% | 93.8% | (many diffs) |
| 37 | mouse | 15270 | H2afx | H2A histone family, member X | NM_010436.2 | 76.1% | 81.9% | (many diffs) |
| 38 | mouse | 667728 | Hist1h2al | histone cluster 1, H2al | NR_132443.1 | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 453
- ORF length:
- 387
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cggtcgcggg aaacagggcg gcaaagtgcg agcaaaggcc aaatcccgct 121 ccTCCCGCGC GGGCCTGCAG TTCCCGGTGG GCCGAGTGCA CAGACTGCTG CGCAAAGGGA 181 ACTACGCGGA GCGAGTGGGC GCCGGGGCGC CGGTGTACCT GGCGGCGGTG TTGGAGTACC 241 TTACGGCGGA GATCCTGGAG CTGGCTGGCA ACGCCGCGCG TGACAACAAG AAGACCAGGA 301 TAATTCCCCG CCACCTGCAG CTCGCCATCC GCAACGACGA GGAGTTAAAC AAGCTGCTGG 361 GCAAAGTGAC CATCGCTCAG GGCGGCGTCC TGCCCAACAT CCAGGCCGTG CTGCTGCCCA 421 AGAAGACGGA GAGTCAGAAG ACGAAGAGCA AATGCCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTTGTGG AAAGGACGAA 601 CAGCCCAAGT ATGTTGCTGT GATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 661 tttgtgaaag att