Construct: ORF TRCN0000479002
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005343.2_s317c1
- Derived from:
- ccsbBroadEn_11109
- DNA Barcode:
- ATGGCACAGCTAGAATGACTTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KB1 (6198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479002
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001272043.2 | 100% | 100% | |
| 2 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_003161.4 | 85.7% | 85.1% | (many diffs) |
| 3 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | XM_011525102.3 | 84.5% | 84.2% | (many diffs) |
| 4 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001272042.1 | 81.3% | 80.7% | (many diffs) |
| 5 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001272060.1 | 81.3% | 80.7% | (many diffs) |
| 6 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369669.1 | 78.8% | 78.2% | (many diffs) |
| 7 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001272044.1 | 75.6% | 75% | (many diffs) |
| 8 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369670.1 | 71.6% | 71% | (many diffs) |
| 9 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369671.1 | 70.4% | 69.9% | (many diffs) |
| 10 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369672.1 | 68.7% | 68.1% | (many diffs) |
| 11 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369674.1 | 46.3% | 42.3% | (many diffs) |
| 12 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369675.1 | 46.3% | 42.3% | (many diffs) |
| 13 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369676.1 | 44.9% | 42.5% | (many diffs) |
| 14 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369677.1 | 44.9% | 42.5% | (many diffs) |
| 15 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369678.1 | 44.9% | 42.5% | (many diffs) |
| 16 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369679.1 | 44.9% | 42.5% | (many diffs) |
| 17 | human | 100287072 | LOC100287072 | ribosomal protein S6 kinase... | NR_073509.1 | 34.8% | (many diffs) | |
| 18 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NM_001369673.1 | 33.2% | 31% | (many diffs) |
| 19 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161461.1 | 24.8% | (many diffs) | |
| 20 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161456.1 | 24.8% | (many diffs) | |
| 21 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161459.1 | 24.4% | (many diffs) | |
| 22 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161458.1 | 24% | (many diffs) | |
| 23 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161460.1 | 23.5% | (many diffs) | |
| 24 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161457.1 | 23% | (many diffs) | |
| 25 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161462.1 | 22.8% | (many diffs) | |
| 26 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | NR_161455.1 | 22.1% | (many diffs) | |
| 27 | human | 6198 | RPS6KB1 | ribosomal protein S6 kinase B1 | XR_002958051.1 | 17.5% | (many diffs) | |
| 28 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_011249275.2 | 94.4% | 98.6% | (many diffs) |
| 29 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_011249276.2 | 89.6% | 91.1% | (many diffs) |
| 30 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | NM_001114334.1 | 81.3% | 84.7% | (many diffs) |
| 31 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_006534314.3 | 71.8% | 75% | (many diffs) |
| 32 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_006534315.3 | 66.2% | 69.5% | (many diffs) |
| 33 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_017314791.1 | 55.3% | 39.8% | (many diffs) |
| 34 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_017314792.1 | 47% | 40.2% | (many diffs) |
| 35 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_006534317.2 | 44.9% | 46.4% | (many diffs) |
| 36 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_011249277.2 | 44.9% | 46.4% | (many diffs) |
| 37 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XM_017314793.1 | 42.5% | 42.7% | (many diffs) |
| 38 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XR_879743.2 | 28.9% | (many diffs) | |
| 39 | mouse | 72508 | Rps6kb1 | ribosomal protein S6 kinase... | XR_001780060.1 | 23.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1419
- ORF length:
- 1353
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcgacgaagg aggcgggacg gcttttaccc agccccggac ttccgagaca 121 gggaagctga ggacatggca ggagtgtttg acatagacct ggaccagcca gaggacgcgg 181 gctctgagga tgagctggag gaggggggtc agttaaatga aagcatggac catgggggag 241 ttggaccata tgaacttggc atggaacatt gtgagaaatt tgaaatctca gaaactagtg 301 tgaacagagg gccagaaaaa atcagaccag aatgttttga gctacttcgg gtacttggta 361 aagggggcta tggaaaggtt tttcaagtac gaaaagtaac aggagcaaat actgggaaaa 421 tatttgccat gaaggtgctt aaaaaggcaa tgatagtaag aaatgctaaa gatacagctc 481 atacaaaagc agaacggaat attctggagg aagtaaagca tcccttcatc gtggatttaa 541 tttatgcctt tcagactggt ggaaaactct acctcatcct tgagtatctc agtggaggag 601 aactatttat gcagttagaa agagagggaa tatttatgga agacactgcc tgcttttact 661 tggcagaaat ctccatggct ttggggcatt tacatcaaaa ggggatcatc tacagagacc 721 tgaagccgga gaatatcatg cttaatcacc aaggtcatgt gaaactaaca gactttggac 781 tatgcaaaga atctattcat gatggaacag tcacacacac attttgtgga acaatagaat 841 acatggcccc tgaaatcttg atgagaagtg gccacaatcg tgctgtggat tggtggagtt 901 tgggagcatt aatgtatgac atgctgactg gagcaccccc attcactggg gagaatagaa 961 agaaaacaat tgacaaaatc ctcaaatgta aactcaattt gcctccctac ctcacacaag 1021 aagccagaga tctgcttaaa aagctgctGA AAAGAAATGC TGCTTCTCGT CTGGGAGCTG 1081 GTCCTGGGGA CGCTGGAGAA GTTCAAGCTC ATCCATTCTT TAGACACATT AACTGGGAAG 1141 AACTTCTGGC TCGAAAGGTG GAGCCCCCCT TTAAACCTCT GTTGCAATCT GAAGAGGATG 1201 TAAGTCAGTT TGATTCCAAG TTTACACGTC AGACACCTGT CGACAGCCCA GATGACTCAA 1261 CTCTCAGTGA AAGTGCCAAT CAGGTCTTTC TGGGTTTTAC ATATGTGGCT CCATCTGTAC 1321 TTGAAAGTGT GAAAGAAAAG TTTTCCTTTG AACCAAAAAT CCGATCACCT CGAAGATTTA 1381 TTGGCAGCCC ACGAACACCT GTCAGTACTG CTATGTGCTA CCCAACTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGAATGGCA CAGCTAGAAT GACTTCCCAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt