Transcript: Human NR_161461.1

Homo sapiens ribosomal protein S6 kinase B1 (RPS6KB1), transcript variant 22, non-coding RNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
RPS6KB1 (6198)
Length:
5432
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_161461.1
NBCI Gene record:
RPS6KB1 (6198)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_161461.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196342 GCATGTATGATACTCCATAAA pLKO.1 4621 3UTR 100% 13.200 18.480 N RPS6KB1 n/a
2 TRCN0000350402 TTTGCCATGAAGGTGCTTAAA pLKO_005 422 3UTR 100% 13.200 18.480 N RPS6KB1 n/a
3 TRCN0000003161 TATTTGCCATGAAGGTGCTTA pLKO.1 420 3UTR 100% 4.950 3.465 N RPS6KB1 n/a
4 TRCN0000195109 CCGGAGAATATCATGCTTAAT pLKO.1 725 3UTR 100% 13.200 7.920 N RPS6KB1 n/a
5 TRCN0000022904 GCATGGAACATTGTGAGAAAT pLKO.1 258 3UTR 100% 13.200 7.920 N Rps6kb1 n/a
6 TRCN0000297869 GCATGGAACATTGTGAGAAAT pLKO_005 258 3UTR 100% 13.200 7.920 N Rps6kb1 n/a
7 TRCN0000003162 GCATCGGCACCACTTCCAATA pLKO.1 1542 3UTR 100% 10.800 6.480 N RPS6KB1 n/a
8 TRCN0000315004 GCGACATCTTCTCAACCTTAT pLKO_005 1884 3UTR 100% 10.800 6.480 N RPS6KB1 n/a
9 TRCN0000315003 ATCCGATCACCTCGAAGATTT pLKO_005 1362 3UTR 100% 13.200 6.600 Y RPS6KB1 n/a
10 TRCN0000195341 CCAAGGTCATGTGAAACTAAC pLKO.1 748 3UTR 100% 10.800 5.400 Y RPS6KB1 n/a
11 TRCN0000194766 CCTATAATACTTGCAACTAAG pLKO.1 2031 3UTR 100% 10.800 5.400 Y RPS6KB1 n/a
12 TRCN0000197241 GCAATCTGAAGAGGATGTAAG pLKO.1 1187 3UTR 100% 10.800 5.400 Y RPS6KB1 n/a
13 TRCN0000022907 GCAGTTAGAAAGAGAGGGAAT pLKO.1 610 3UTR 100% 4.050 2.025 Y Rps6kb1 n/a
14 TRCN0000280573 GCAGTTAGAAAGAGAGGGAAT pLKO_005 610 3UTR 100% 4.050 2.025 Y Rps6kb1 n/a
15 TRCN0000003159 AGCACAGCAAATCCTCAGACA pLKO.1 1458 3UTR 100% 2.640 1.320 Y RPS6KB1 n/a
16 TRCN0000315001 AGCACAGCAAATCCTCAGACA pLKO_005 1458 3UTR 100% 2.640 1.320 Y RPS6KB1 n/a
17 TRCN0000003158 CCCATGATCTCCAAACGGCCA pLKO.1 1602 3UTR 100% 0.180 0.090 Y RPS6KB1 n/a
18 TRCN0000314934 CCCATGATCTCCAAACGGCCA pLKO_005 1602 3UTR 100% 0.180 0.090 Y RPS6KB1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_161461.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489919 CGAACCTTCGACTGCACGAATTCC pLX_317 24.5% 29% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_11109 pDONR223 100% 24.8% None (many diffs) n/a
3 ccsbBroad304_11109 pLX_304 0% 24.8% V5 (many diffs) n/a
4 TRCN0000479002 ATGGCACAGCTAGAATGACTTCCC pLX_317 27.7% 24.8% V5 (many diffs) n/a
5 ccsbBroadEn_14833 pDONR223 0% 24.8% None (many diffs) n/a
6 ccsbBroad304_14833 pLX_304 49.9% 24.8% V5 (many diffs) n/a
7 TRCN0000481178 ACCACACGATGAGTAGACCGGCCA pLX_317 32.8% 24.8% V5 (many diffs) n/a
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