Construct: ORF TRCN0000479222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010293.1_s317c1
- Derived from:
- ccsbBroadEn_13399
- DNA Barcode:
- TCAAGCATTTAAGTTGAAATTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM268 (203197)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479222
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_011518355.1 | 100% | 100% | |
| 2 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_011518354.1 | 99.6% | 99.6% | 151_153delCAG |
| 3 | human | 203197 | TMEM268 | transmembrane protein 268 | NM_153045.4 | 91.8% | 90.9% | (many diffs) |
| 4 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_024447441.1 | 91.8% | 90.9% | (many diffs) |
| 5 | human | 203197 | TMEM268 | transmembrane protein 268 | NM_001330760.2 | 91.5% | 90.6% | (many diffs) |
| 6 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_017014430.1 | 88.4% | 88.4% | 409_410ins111 |
| 7 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_011518352.1 | 77.7% | 75.6% | (many diffs) |
| 8 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_011518350.1 | 77.5% | 75.4% | (many diffs) |
| 9 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_011518351.2 | 77.5% | 75.4% | (many diffs) |
| 10 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_017014429.2 | 77.5% | 75.4% | (many diffs) |
| 11 | human | 203197 | TMEM268 | transmembrane protein 268 | XM_011518353.1 | 68.5% | 66.5% | (many diffs) |
| 12 | human | 203197 | TMEM268 | transmembrane protein 268 | XR_001746226.1 | 25.6% | (many diffs) | |
| 13 | human | 203197 | TMEM268 | transmembrane protein 268 | XR_001746225.1 | 24.6% | (many diffs) | |
| 14 | mouse | 230279 | Tmem268 | transmembrane protein 268 | NM_144905.3 | 81.3% | 81.9% | (many diffs) |
| 15 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_006537866.2 | 81.3% | 81.9% | (many diffs) |
| 16 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_006537867.2 | 81.3% | 81.9% | (many diffs) |
| 17 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_006537868.3 | 81.3% | 81.9% | (many diffs) |
| 18 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_006537869.1 | 81.3% | 81.9% | (many diffs) |
| 19 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_006537870.3 | 81.3% | 81.9% | (many diffs) |
| 20 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_006537871.3 | 81.3% | 81.9% | (many diffs) |
| 21 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_006537872.3 | 81.3% | 81.9% | (many diffs) |
| 22 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_006537873.3 | 81.3% | 81.9% | (many diffs) |
| 23 | mouse | 230279 | Tmem268 | transmembrane protein 268 | XM_011250009.2 | 81.3% | 81.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1032
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggttatcact cagccagatg aagcttctgg tctgcttcca gagctccaca 121 atggccaggt cctcactgtt ctccggattg acaatacctg tgcacccatc tccttcgacc 181 tgggagccgc agaagagcaa ctgcaaactt ggggcatcca ggtcccggct gaccagtaca 241 ggagcttggc tgagagtgcc ctcttggagc cccaagtgag aagatatatc atctacaact 301 cgaggcctat gcggctggcc tttgctgtgg ttttctatgt ggtggtgtgg gccaatatct 361 actctaccag tcagatgttt gccttgggga accactgggc tggcatgctg ctcgtgaccc 421 tggccgcggt gagcctgacc ttgactcttg tgctggtctt tgaaagacac cagaagaagg 481 ccaacaccaa cacggacctg aggctggcag ctgccaatgg agccctcctg agacaCCGGG 541 TGCTGCTGGG GGTGACAGAC ACAGTGGAAG GATGCCAGAG TGTGATTCAG CTTTGGTTTG 601 TCTACTTCGA CCTGGAGAAC TGTGTGCAGT TTTTGTCTGA TCATGTTCAA GAAATGAAGA 661 CTAGCCAAGA GTCCTTGCTG AGAAGCAGAT TGAGCCAGTT GTGTGTTGTC ATGGAGACTG 721 GGGTGAGCCC TGCAACAGCG GAGGGGCCTG AGAACTTGGA GGATGCTCCT CTCCTGCCCG 781 GCAATTCTTG TCCTAACGAG AGGCCACTCA TGCAGACTGA GCTTCATCAG CTTGTTCCTG 841 AGGCTGAGCC GGAGGAAATG GCCCGCCAGC TGCTGGCAGT GTTTGGCGGC TACTACATCC 901 GGCTTCTAGT GACCTCCCAG CTCCCTCAGG CAATGGGGAC ACGACACACG AACTCTCCGA 961 GAATTCCATG CCCCTGCCAG CTCATAGAAG CCTACATCCT AGGCACAGGG TGCTGCCCGT 1021 TCCTGGCGAG GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA AGCATTTAAG TTGAAATTCC 1201 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t