Construct: ORF TRCN0000479480

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013164.2_s317c1
Derived from:
ccsbBroadEn_06790
DNA Barcode:
CATGACTATTACGCGTCAGTGGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSG3 (5671)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479480
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5671 PSG3 pregnancy specific beta-1-g... NM_021016.4 99.9% 99.7% 1234A>G
2 human 440533 PSG8 pregnancy specific beta-1-g... NM_182707.3 93.7% 87.7% (many diffs)
3 human 5669 PSG1 pregnancy specific beta-1-g... NM_001297773.2 93.5% 88% (many diffs)
4 human 440533 PSG8 pregnancy specific beta-1-g... NM_001130167.2 93.3% 87.9% (many diffs)
5 human 5669 PSG1 pregnancy specific beta-1-g... NM_001184825.2 93.2% 88.3% (many diffs)
6 human 5669 PSG1 pregnancy specific beta-1-g... NM_001184826.2 92.9% 88.7% (many diffs)
7 human 5669 PSG1 pregnancy specific beta-1-g... NM_006905.3 92.9% 88.2% (many diffs)
8 human 5672 PSG4 pregnancy specific beta-1-g... XM_017026997.2 92.6% 86.3% (many diffs)
9 human 5675 PSG6 pregnancy specific beta-1-g... NM_001031850.4 92.4% 86.8% (many diffs)
10 human 5676 PSG7 pregnancy specific beta-1-g... NM_002783.3 92.2% 87.2% (many diffs)
11 human 5672 PSG4 pregnancy specific beta-1-g... NM_002780.5 92% 86.5% (many diffs)
12 human 5678 PSG9 pregnancy specific beta-1-g... NM_002784.5 91.8% 83.2% (many diffs)
13 human 5675 PSG6 pregnancy specific beta-1-g... NM_002782.5 90.7% 86% (many diffs)
14 human 5678 PSG9 pregnancy specific beta-1-g... XM_017027007.2 90.5% 83.5% (many diffs)
15 human 5678 PSG9 pregnancy specific beta-1-g... XM_017027004.2 80.8% 73.9% (many diffs)
16 human 5673 PSG5 pregnancy specific beta-1-g... NM_001130014.2 74.4% 70% (many diffs)
17 human 5673 PSG5 pregnancy specific beta-1-g... NM_002781.4 74.4% 70% (many diffs)
18 human 5673 PSG5 pregnancy specific beta-1-g... XM_017027003.1 74.4% 70% (many diffs)
19 human 5671 PSG3 pregnancy specific beta-1-g... XM_011527126.2 74.3% 74% 1_66del;775_776ins279;1021A>G
20 human 5670 PSG2 pregnancy specific beta-1-g... NM_031246.4 72.8% 66.5% (many diffs)
21 human 5669 PSG1 pregnancy specific beta-1-g... NM_001330524.2 72.6% 68.4% (many diffs)
22 human 5669 PSG1 pregnancy specific beta-1-g... XM_005259065.4 72.5% 68.3% (many diffs)
23 human 5680 PSG11 pregnancy specific beta-1-g... NM_002785.3 72.3% 66.3% (many diffs)
24 human 5672 PSG4 pregnancy specific beta-1-g... XM_017027001.2 72.1% 67.4% (many diffs)
25 human 440533 PSG8 pregnancy specific beta-1-g... XM_011526986.1 72% 67% (many diffs)
26 human 5672 PSG4 pregnancy specific beta-1-g... XM_017027002.2 71.8% 66% (many diffs)
27 human 5669 PSG1 pregnancy specific beta-1-g... XM_011527125.2 71.7% 67.2% (many diffs)
28 human 5672 PSG4 pregnancy specific beta-1-g... NM_001276495.1 71.4% 67.5% (many diffs)
29 human 5678 PSG9 pregnancy specific beta-1-g... NM_001301707.2 71.4% 64.5% (many diffs)
30 human 5672 PSG4 pregnancy specific beta-1-g... NM_213633.3 71.1% 66.1% (many diffs)
31 human 5678 PSG9 pregnancy specific beta-1-g... NM_001301708.2 71% 63.1% (many diffs)
32 human 440533 PSG8 pregnancy specific beta-1-g... NM_001130168.2 66.7% 62.8% (many diffs)
33 human 5676 PSG7 pregnancy specific beta-1-g... NM_001206650.2 65.9% 62.6% (many diffs)
34 human 5672 PSG4 pregnancy specific beta-1-g... NM_001316339.2 65.8% 61.4% (many diffs)
35 human 440533 PSG8 pregnancy specific beta-1-g... XM_011526987.3 65.2% 59.8% (many diffs)
36 human 5678 PSG9 pregnancy specific beta-1-g... XM_017027005.2 63.1% 57.3% (many diffs)
37 human 5678 PSG9 pregnancy specific beta-1-g... XM_005259075.4 62.8% 56.1% (many diffs)
38 human 440533 PSG8 pregnancy specific beta-1-g... XM_011526988.2 57.9% 54.9% (many diffs)
39 human 5671 PSG3 pregnancy specific beta-1-g... XM_011527127.2 54.3% 53.5% (many diffs)
40 human 5678 PSG9 pregnancy specific beta-1-g... NM_001301709.2 50.8% 44.8% (many diffs)
41 human 653492 PSG10P pregnancy specific beta-1-g... NR_026824.1 46% (many diffs)
42 human 5678 PSG9 pregnancy specific beta-1-g... XM_017027006.2 45.1% 40% (many diffs)
43 human 5680 PSG11 pregnancy specific beta-1-g... NM_001113410.2 45.1% 39.9% (many diffs)
44 human 5680 PSG11 pregnancy specific beta-1-g... NM_203287.2 45.1% 39.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1350
ORF length:
1284
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gcccctctca gcccctccct gcacacagcg catcacctgg aaggggctcc
121tgctcacagc attactttta aacttctgga acttgcctac cactgcccaa gtcacgattg
181aagccgagcc aaccaaagtt tccaagggga aggacgttct tctacttgtc cacaatttgc
241cccagaatct tgctggctac atctggtaca aagggcaaat gaaggacctc taccattaca
301ttacatcata cgtagtagat ggtcaaataa ttatatatgg gcctgcatac agtggacgag
361aaacagtata ttccaatgca tccctgctga tccagaatgt cacccgggag gacgcaggat
421cctacacctt acacatcgta aagcgaggtg atgggactag aggagaaact ggacatttca
481ccttcacctt atacctggag actcccaagc cctccatctc cagcagcaac ttatacccca
541gggaggacat ggaggctgtg agcttaacct gtgatcctga gactccggac gcaagctacc
601tgtggtggat gaatggtcag agcctcccta tgactcacag cttgcagttg tccaaaaaca
661aaaggaccct ctttctattt ggtgtcacaa agtacactgc aggaccctat gaatgtgaaa
721tacggaaccc agtgagtgcc agccgcagtg acccagtcac cctgaatctc ctcccgaagc
781tgcccaagcc ctacatcacc atcaacaact taaaccccag ggagaataag gatgtcttag
841ccttcacctg tgaacctaag agtgagaact acacctacat ttggtggcta aatggtcaga
901gcctcccggt cagtcccagg gtaaagcgac ccattgaaaa caggatcctc attctaccca
961gtgtcacgag aaatgaaaca ggaccctatc aatgtgaaat acaggaccga tatggtggca
1021tccgcagtta cccagtcacc ctgaatgtcc tctatggtcc agacctcccc agaatttacc
1081cttcattcac ctattaccat tcaggagaaa acctctactt gtcctgcttc gcggactcta
1141acccaccagc agaatattct tggacaatta atgGGAAGTT TCAGCTATCA GGACAAAAGC
1201TCTTTATCCC CCAGATTACT ACAAAGCATA GCGGGCTCTA TGCTTGCTCT GTTCGTAACT
1261CAGCCACTGG CATGGAAAGC TCCAAATCCA TGACAGTCGA AGTCTCTGCT CCTTCAGGAA
1321CAGGACATCT TCCTGGCCTT AATCCATTAT ACCCAACTTT CTTGTACAAA GTGGTTGATA
1381TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1441CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACATGA
1501CTATTACGCG TCAGTGGATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1561tgaaagatt

Download FASTA (ORF) (Full)