Construct: ORF TRCN0000479509
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014640.1_s317c1
- Derived from:
- ccsbBroadEn_14276
- DNA Barcode:
- CTTACGGTTTCCACAAAGGTCACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDZD7 (79955)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479509
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79955 | PDZD7 | PDZ domain containing 7 | NM_024895.4 | 98.6% | 98.4% | 1341_1343delGAA;1532_1534delTTTinsCAA;1537_1551del |
| 2 | human | 79955 | PDZD7 | PDZ domain containing 7 | XM_017016668.2 | 96.3% | 83.6% | (many diffs) |
| 3 | human | 79955 | PDZD7 | PDZ domain containing 7 | XM_011540183.3 | 95.4% | 83.3% | (many diffs) |
| 4 | human | 79955 | PDZD7 | PDZ domain containing 7 | NM_001351044.1 | 94.6% | 82.4% | (many diffs) |
| 5 | human | 79955 | PDZD7 | PDZ domain containing 7 | XM_011540181.3 | 93.5% | 93.1% | (many diffs) |
| 6 | human | 79955 | PDZD7 | PDZ domain containing 7 | XM_005270165.3 | 90.8% | 90.1% | (many diffs) |
| 7 | human | 79955 | PDZD7 | PDZ domain containing 7 | XM_017016667.2 | 89% | 88.3% | (many diffs) |
| 8 | human | 79955 | PDZD7 | PDZ domain containing 7 | XR_945816.3 | 77.5% | (many diffs) | |
| 9 | human | 79955 | PDZD7 | PDZ domain containing 7 | XM_011540179.3 | 75.1% | 74.8% | (many diffs) |
| 10 | human | 79955 | PDZD7 | PDZ domain containing 7 | NM_001195263.2 | 49.2% | 49% | (many diffs) |
| 11 | human | 79955 | PDZD7 | PDZ domain containing 7 | XM_011540177.3 | 49.2% | 49% | (many diffs) |
| 12 | human | 79955 | PDZD7 | PDZ domain containing 7 | XM_011540178.3 | 49.2% | 49% | (many diffs) |
| 13 | human | 79955 | PDZD7 | PDZ domain containing 7 | XR_001747203.2 | 47.6% | (many diffs) | |
| 14 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_017318033.1 | 80.6% | 79% | (many diffs) |
| 15 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_017318034.1 | 80.1% | 78.8% | (many diffs) |
| 16 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_006526542.1 | 78.9% | 84.2% | (many diffs) |
| 17 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_006526545.3 | 75.5% | 80.4% | (many diffs) |
| 18 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_006526540.3 | 74.6% | 79.5% | (many diffs) |
| 19 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_006526541.3 | 66.8% | 74.5% | (many diffs) |
| 20 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_006526539.3 | 65.1% | 69.1% | (many diffs) |
| 21 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XR_386435.2 | 60.7% | (many diffs) | |
| 22 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | NM_001195265.1 | 43.2% | 45.8% | (many diffs) |
| 23 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_006526536.1 | 43.2% | 45.8% | (many diffs) |
| 24 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_006526537.3 | 43.2% | 45.8% | (many diffs) |
| 25 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XM_006526538.1 | 39.3% | 42.3% | (many diffs) |
| 26 | mouse | 100503041 | Pdzd7 | PDZ domain containing 7 | XR_877655.1 | 37.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagggtttc gcagtgggct tcgacccact gggcctagga gacctgagct 121 ccggctctct gagctccctc tcctcccgag gccacctagg cagcgactca ggctccaccg 181 caacgcgata cctgctaagg aagcaacaac ggctgctgaa cgggcccccc cgcggaatcc 241 gagcctcatc gcccatggga cgcgtcatcc tcatcaactc ccccatcgaa gccaacagtg 301 atgaaagtga catcatccat tcagtccggg tggagaagag tccagcaggg aggctgggct 361 tcagcgtgcg cgggggctca gagcatggcc tgggcatctt cgtcagcaaa gtggaggaag 421 gcagcagtgc agagcgggct ggcctgtgcg tgggggacaa gatcacggag gtgaatgggc 481 tgagcctgga gagcaccacc atgggtagcg ccgtaaaggt gctgaccagc agcagccgcc 541 tgcacatgat ggttcggcgc atgggccgtg tgccgggcat caagttctcc aaggagaaga 601 ccacgtgggt ggatgtggtg aatcggcgcc tggtagtgga gaagtgcggt tcaacaccct 661 ccgacaccag ctcagaagat ggtgtccggc gcatcgtcca cctatacaca acctccgacg 721 acttctgcct gggcttcaac atccgtgggg gcaaggagtt tggcctgggc atctatgtgt 781 ccaaagtgga ccatggtggg ctggccgagg agaatggcat caaggtgggg gaccaggtcc 841 tggcagccaa cggtgtcagg tttgacgaca tcagccacag ccaggccgtg gaggtgctga 901 agggccaaac gcacatcatg ctgaccatca aggagaccgg ccggtatcct gcctacaagg 961 agatggtttc tgagtactgc tggctggacc gactgagcaa cggggtgctg cagcagctgt 1021 ccccggcctc tgagagcagc tccagcgtct cttcgtgcgc ctccagcgcc ccctacagct 1081 cgggctccct gccgtcggac cgcatggaca tctgcctcgg gcaggaggag cccggcagcc 1141 gcggcccagg ctgggggcgg gcggacacag ccatgcagac ggagcccgat gcgggaggcc 1201 gggtggagac ctggtgcagc gtgcggccca cagtcatcct cagggacacc gccatccgct 1261 cggacggccc ccatcccggc cgccgccttg actctgcgct ctctgagtct cccaagacgg 1321 ctttgctgct ggcccTCAGC CGACCCCGGC CCCCCATCAC GCGCTCCCAG AGCTATCTGA 1381 CCTTGTGGGA GGAGAAGCAG CAGCGGAAGG AGAAGTCGGG GTCCCCTGGG GAGAAGGGTG 1441 CCCTGCAGCG CTCCAAGACG CTGATGAACC TCTTCTTCAA GGGAGGGCGG CAGGGGAGGC 1501 TAGCGCGGGA CGGGCGCAGA GAGGCCTGGA CACTGGACAG CGGGAGCCTG GCCAAAACTT 1561 ACCCTCGCCT GGACATAGAG AAAGAGATGG GGGCAACATA ACCAACTTTC TTGTACAAAG 1621 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1681 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1741 ACGACTTACG GTTTCCACAA AGGTCACCAC GCGTTAAGTC gacaatcaac ctctggatta 1801 caaaatttgt gaaagatt