Construct: ORF TRCN0000481587
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009553.1_s317c1
- Derived from:
- ccsbBroadEn_08342
- DNA Barcode:
- CCCCTTCTTGCTCGCATTGACCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAPGEF6 (51735)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481587
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51735 | RAPGEF6 | Rap guanine nucleotide exch... | NM_001164388.2 | 99.9% | 100% | 252G>A |
| 2 | human | 51735 | RAPGEF6 | Rap guanine nucleotide exch... | NM_001164387.2 | 99.6% | 99.6% | 252G>A;2240_2254del |
| 3 | human | 51735 | RAPGEF6 | Rap guanine nucleotide exch... | NM_001164386.2 | 93% | 92.8% | (many diffs) |
| 4 | human | 51735 | RAPGEF6 | Rap guanine nucleotide exch... | NM_016340.6 | 92.5% | 92.3% | (many diffs) |
| 5 | human | 51735 | RAPGEF6 | Rap guanine nucleotide exch... | NM_001164389.2 | 88.8% | 84.7% | (many diffs) |
| 6 | human | 51735 | RAPGEF6 | Rap guanine nucleotide exch... | NM_001164390.2 | 54.9% | 54.9% | 252G>A;2481_2482ins2031 |
| 7 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532564.3 | 89% | 91.7% | (many diffs) |
| 8 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | NM_001252496.1 | 88.7% | 91.4% | (many diffs) |
| 9 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_017314348.1 | 88.2% | 90.8% | (many diffs) |
| 10 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532561.3 | 83.1% | 85.4% | (many diffs) |
| 11 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532560.3 | 82.9% | 85.1% | (many diffs) |
| 12 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | NM_175258.4 | 82.6% | 84.9% | (many diffs) |
| 13 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | NM_001252494.1 | 82.4% | 84.6% | (many diffs) |
| 14 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532567.3 | 80.6% | 78.7% | (many diffs) |
| 15 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532568.3 | 80.4% | 78.5% | (many diffs) |
| 16 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532562.2 | 80.2% | 82.2% | (many diffs) |
| 17 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532563.3 | 77.5% | 78.6% | (many diffs) |
| 18 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532566.3 | 74.8% | 75.2% | (many diffs) |
| 19 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | XM_006532565.3 | 74.5% | 74.9% | (many diffs) |
| 20 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | NM_001252497.1 | 36.1% | 36.2% | (many diffs) |
| 21 | mouse | 192786 | Rapgef6 | Rap guanine nucleotide exch... | NM_001252498.1 | 25.9% | 25.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4581
- ORF length:
- 4512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaactcaccc gtggaccctg gcgctaggca ggcgttgagg aagaagccac 121 ccgagcggac tcccgaggac ttaaatacta tttattctta tcttcatgga atggaaatat 181 tatcaaatct cagggaacat cagcttagat taatgtctgc aagagcacgc tatgagagat 241 acagtggcaa tcaggttctc ttttgttcag aaacgattgc cagatgttgg tatatcctac 301 tttctggatc tgtgcttgta aaaggctcca tggtcttgcc tccttgcagt tttggtaagc 361 agtttggagg aaaaagagga tgtgattgtc ttgtattaga gccttcagaa atgattgtgg 421 tagagaatgc caaagataat gaagatagta ttctacaaag agaaattcct gccagacaat 481 cccgaagaag atttcggaaa attaactata aaggagagcg ccaaaccatt actgatgatg 541 tggaggttaa cagctatctt tctcttccag ctgatcttac caagatgcat ctcacagaaa 601 accctcatcc acaggtgact catgtgtctt ctagtcagtc tggttgtagc attgccagtg 661 actctggaag cagcagttta tctgatatct atcaggctac ggagagtgag gtaggagatg 721 tagatttgac acgtcttcca gaaggacctg ttgattctga ggatgacgaa gaggaagatg 781 aagagattga tcgaacagat ccattgcagg ggcgagatct tgttcgagaa tgtcttgaaa 841 aagaacctgc agacaaaact gatgatgaca ttgaacaatt gctggagttt atgcaccagc 901 tccctgcatt tgcaaacatg accatgtctg taaggagaga actctgctca gtgatgattt 961 ttgaagtggt agagcaggct ggagctatta ttcttgaaga tgggcaagag cttgactcat 1021 ggtatgttat tttaaacggc actgtggaaa tcagtcatcc agatggaaaa gttgaaaatt 1081 tgtttatggg aaatagtttt ggaattactc ccactctgga taagcagtac atgcatggaa 1141 ttgtcaggac taaagtagat gattgtcagt ttgtctgcat agcccagcaa gattattgga 1201 gaattttaaa ccatgtggaa aaaaataccc ataaagttga ggaagaggga gaaattgtta 1261 tggtacatga gcatcgggaa ctagaccgga gtggaaccag gaaaggacac attgtgatca 1321 aggcaacacc tgagcgtctc ataatgcatt taatagaaga acattccatc gtggatccaa 1381 cttatataga agattttcta ttaacttaca ggacatttct tgaaagtcct ttggatgttg 1441 ggatcaaact attggaatgg tttaagatcg acagcttaag agataaggtg acacggattg 1501 tattattatg ggtaaataat cattttaatg attttgaagg tgaccctgct atgactcgat 1561 ttctagagga atttgaaaaa aatctggaag atacaaagat gaatggtcat ctccggttat 1621 tgaatattgc ctgtgctgca aaggctaagt ggagacaggt tgtgctgcaa aaggcttccc 1681 gcgagtcccc tctacaattc agccttaatg gagggagtga gaagggattt ggtatttttg 1741 ttgaaggagt agaacctggt agcaaagctg ctgattcagg actgaaacgt ggtgatcaga 1801 ttatggaagt aaatggacaa aactttgaga atattacatt tatgaaagcc gttgaaattt 1861 tgaggaataa tactcatctt gcacttactg tgaagaccaa catttttgtg ttcaaagagt 1921 tactttttag gactgaacaa gagaaatctg gtgttcctca tattcccaaa attgctgaaa 1981 aaaaaagtaa tcgccattct atccagcatg tgccaggaga tattgaacag acatcacagg 2041 agaaaggaag taagaaagtt aaagcaaata ctgtttcagg tggaagaaac aaaatcagga 2101 agattttgga taaaacacga tttagtatct tgcctccaaa gctatttagt gatggaggcc 2161 taagccaatc acaagatgac agcattgtgg gaacaaggca ctgtaggcat agtctggcta 2221 taatgcccat ccctggaaca ctctcatcca gcagccctga tctcctgcag cctaccacca 2281 gtatgttgga tttttccaat ccttcagata tccctgatca agttataaga gttttcaaag 2341 tggatcagca aagttgctac attatcatca gtaaagacac cacagctaaa gaagtagttt 2401 ttcatgctgt tcatgaattt ggtttgaccg gtgcatccga cacatattct ctctgtgaag 2461 tttctgttac tcctgagggt gtcataaaac agagaagact tccagatcag ttctccaaat 2521 tagctgatag aattcaactc aatggaaggt attacttaaa aaataacatg gaaacagaaa 2581 ccttatgttc agatgaagat gctcaagaac tagttaagga aagccagcta tccatgctgc 2641 agctcagtac cattgaggtg gccacccagc tgtcaatgag ggactttgat ttgtttcgta 2701 atattgaacc gactgagtac atcgatgacc tttttaagtt aaattccaaa acaggaaata 2761 ctcatttgaa gaggtttgag gacattgtaa accaagagac attctgggtt gcctcagaaa 2821 ttttaactga agcaaatcag ctcaaacgaa tgaagattat taagcatttt attaaaattg 2881 cacttcattg tcgagaatgt aagaacttca attccatgtt tgcaataata agtggcttga 2941 acctggcatc tgtagcaaga ctcagaggaa cttgggaaaa gttaccaagc aaatacgaga 3001 aacatcttca agatctacaa gacatttttg atccatctag aaacatggca aagtatagaa 3061 atattcttag tagtcaaagt atgcagcctc caattattcc actcttccct gttgtcaaga 3121 aagatatgac atttctacat gaaggaaatg actccaaagt agatggttta gtaaactttg 3181 agaagttaag aatgatttcc aaggaaatcc gccaagttgt tcgaatgact tctgctaaca 3241 tggacccagc tatgatgttt cgacagagga agaagaggtg gcggagtctg gggtcactga 3301 gtcaaggaag cacaaattca aacatgctgg atgttcaggg aggtgctcac aaaaaaaggg 3361 cacgccgcag ctctctgctt aatgccaaga agctatatga ggatgcccaa atggcaagga 3421 aggtgaagca gtatctttcc agtctcgatg tagagacaga tgaggagaag ttccagatga 3481 tgtcattaca gtgggagcct gcatatggta ccttgaccaa gaatttaagt gagaaaagat 3541 cagccaaatc atctgaaatg tctccagtgc ctatgaggtc agctggccaa acaactaaag 3601 cccacttgca tcaaccccac agagtaagcc aggtgcttca ggtgccagct gttaatttgc 3661 accccatcag gaagaaggga caaacaaaag accctgcact gaatacaagt ttacctcaga 3721 aagttttagg aacaactgaa gaaataagtg gtaagaagca tacagaagac actatttctg 3781 tggcgtcatc tttacattct agtcctcctg catctcctca aggctcccct cacaaaggtt 3841 acacacttat tccatcagct aaatctgaca acttgtctga ctccagccat agtgagattt 3901 cttcacggtc cagcatcgtg agcaattgtt ctgttgactc catgtctgca gctctacagg 3961 atgaacggtg ttcctctcag gccctggcag tccctgaatc cactggggca ttggaaaaga 4021 cagagcacgc ttcagggata ggagatcata gtcaacatgg ccctgggtgg acactcttga 4081 agccatctct aatCAAGTGT TTAGCTGTCT CATCGTCTGT GAGCAATGAA GAGATTTCTC 4141 AAGAGCATAT CATTATAGAA GCAGCTGACA GTGGTCGTGG AAGTTGGACT TCGTGTTCAA 4201 GCAGCTCCCA TGACAACTTC CAAAGCCTTC CAAACCCAAA AAGCTGGGAT TTTTTGAACT 4261 CTTACAGACA TACCCATTTG GATGACCCCA TTGCTGAAGT TGAACCCACT GACTCTGAGC 4321 CCTATTCCTG TTCTAAAAGC TGCTCTAGAA CTTGTGGGCA GTGTAAAGGA AGCCTAGAGA 4381 GAAAGAGTTG GACCTCCTCC AGTTCTCTGT CTGACACGTA TGAACCAAAC TATGGGACAG 4441 TTAAACAGAG AGTATTGGAG AGCACCCCAG CTGAGTCATC TGAAGGCTTG GACCCCAAGG 4501 ATGCCACTGA CCCAGTTTAT AAAACTGTCA CTTCAAGTAC AGAAAAGGGC TTGATTGAAA 4561 ATGAACAAGT TTCAGCAGTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 4621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 4681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCCC TTCTTGCTCG 4741 CATTGACCTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt