Construct: ORF TRCN0000487806

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020714.1_s317c1
DNA Barcode:
ATCGTTGCGGCTGACGACGATGAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
BRD4 (23476)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487806
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23476 BRD4 bromodomain containing 4 NM_001330384.2 100% 100%
2 human 23476 BRD4 bromodomain containing 4 NM_014299.2 90.8% 90.8% 2159_2159delGinsCT;2161C>T;2166_2166delCins216
3 human 23476 BRD4 bromodomain containing 4 XM_011527856.3 90.8% 90.8% 2159_2159delGinsCT;2161C>T;2166_2166delCins216
4 human 23476 BRD4 bromodomain containing 4 NM_058243.2 56.5% 52.2% (many diffs)
5 human 23476 BRD4 bromodomain containing 4 XM_011527854.2 56.5% 52.2% (many diffs)
6 mouse 57261 Brd4 bromodomain containing 4 NM_198094.2 81.5% 87.4% (many diffs)
7 mouse 57261 Brd4 bromodomain containing 4 XM_006524695.3 81.4% 87.3% (many diffs)
8 mouse 57261 Brd4 bromodomain containing 4 XM_006524691.1 50.6% 50.1% (many diffs)
9 mouse 57261 Brd4 bromodomain containing 4 XM_006524690.2 50.6% 50.1% (many diffs)
10 mouse 57261 Brd4 bromodomain containing 4 NM_020508.4 49.4% 48.9% (many diffs)
11 mouse 57261 Brd4 bromodomain containing 4 XM_006524688.1 49.4% 48.9% (many diffs)
12 mouse 57261 Brd4 bromodomain containing 4 XM_006524689.2 49.4% 48.9% (many diffs)
13 mouse 57261 Brd4 bromodomain containing 4 NM_001286630.1 49.4% 48.9% (many diffs)
14 mouse 57261 Brd4 bromodomain containing 4 XM_006524683.2 49.4% 48.9% (many diffs)
15 mouse 57261 Brd4 bromodomain containing 4 XM_006524684.2 49.4% 48.9% (many diffs)
16 mouse 57261 Brd4 bromodomain containing 4 XM_006524685.1 49.4% 48.9% (many diffs)
17 mouse 57261 Brd4 bromodomain containing 4 XM_006524686.1 49.4% 48.9% (many diffs)
18 mouse 57261 Brd4 bromodomain containing 4 XM_006524687.2 49.4% 48.9% (many diffs)
19 mouse 57261 Brd4 bromodomain containing 4 XM_006524693.2 39.4% 38.7% (many diffs)
20 mouse 57261 Brd4 bromodomain containing 4 XM_011246543.2 39.4% 38.7% (many diffs)
21 mouse 57261 Brd4 bromodomain containing 4 XM_017317588.1 39.4% 38.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2454
ORF length:
2382
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtctgcg gagagcggcc ctgggacgag attgagaaat ctgccagtaa
121tgggggatgg actagaaact tcccaaatgt ctacaacaca ggcccaggcc caaccccagc
181cagccaacgc agccagcacc aaccccccgc ccccagagac ctccaaccct aacaagccca
241agaggcagac caaccaactg caatacctgc tcagagtggt gctcaagaca ctatggaaac
301accagtttgc atggcctttc cagcagcctg tggatgccgt caagctgaac ctccctgatt
361actataagat cattaaaacg cctatggata tgggaacaat aaagaagcgc ttggaaaaca
421actattactg gaatgctcag gaatgtatcc aggacttcaa cactatgttt acaaattgtt
481acatctacaa caagcctgga gatgacatag tcttaatggc agaagctctg gaaaagctct
541tcttgcaaaa aataaatgag ctacccacag aagaaaccga gatcatgata gtccaggcaa
601aaggaagagg acgtgggagg aaagaaacag ggacagcaaa acctggcgtt tccacggtac
661caaacacaac tcaagcatcg actcctccgc agacccagac ccctcagccg aatcctcctc
721ctgtgcaggc cacgcctcac cccttccctg ccgtcacccc ggacctcatc gtccagaccc
781ctgtcatgac agtggtgcct ccccagccac tgcagacgcc cccgccagtg cccccccagc
841cacaaccccc acccgctcca gctccccagc ccgtacagag ccacccaccc atcatcgcgg
901ccaccccaca gcctgtgaag acaaagaagg gagtgaagag gaaagcagac accaccaccc
961ccaccaccat tgaccccatt cacgagccac cctcgctgcc cccggagccc aagaccacca
1021agctgggcca gcggcgggag agcagccggc ctgtgaaacc tccaaagaag gacgtgcccg
1081actctcagca gcacccagca ccagagaaga gcagcaaggt ctcggagcag ctcaagtgct
1141gcagcggcat cctcaaggag atgtttgcca agaagcacgc cgcctacgcc tggcccttct
1201acaagcctgt ggacgtggag gcactgggcc tacacgacta ctgtgacatc atcaagcacc
1261ccatggacat gagcacaatc aagtctaaac tggaggcccg tgagtaccgt gatgctcagg
1321agtttggtgc tgacgtccga ttgatgttct ccaactgcta taagtacaac cctcctgacc
1381atgaggtggt ggccatggcc cgcaagctcc aggatgtgtt cgaaatgcgc tttgccaaga
1441tgccggacga gcctgaggag ccagtggtgg ccgtgtcctc cccggcagtg ccccctccca
1501ccaaggttgt ggccccgccc tcatccagcg acagcagcag cgatagctcc tcggacagtg
1561acagttcgac tgatgactct gaggaggagc gagcccagcg gctggctgag ctccaggagc
1621agctcaaagc cgtgcacgag cagcttgcag ccctctctca gccccagcag aacaaaccaa
1681agaaaaagga gaaagacaag aaggaaaaga aaaaagaaaa gcacaaaagg aaagaggaag
1741tggaagagaa taaaaaaagc aaagccaagg aacctcctcc taaaaagacg aagaaaaata
1801atagcagcaa cagcaatgtg agcaagaagg agccagcgcc catgaagagc aagccccctc
1861ccacgtatga gtcggaggaa gaggacaagt gcaagcctat gtcctatgag gagaagcggc
1921agctcagctt ggacatcaac aagctccccg gcgagaagct gggccgcgtg gtgcacatca
1981tccagtcacg ggagccctcc ctgaagaatt ccaaccccga cgagattgaa atcgactttg
2041agaccctgaa gccgtccaca ctgcgtgagc tggagcgcta tgtcacctcc tgtttgcgga
2101agaaaaggaa acctcaagct gagaaagttg atgtgattgc cggctcctcc aagatgaagg
2161gcttctcgtc ctCAGAGTCG GAGAGCTCCA GTGAGTCCAG CTCCTCTGAC AGCGAAGACT
2221CCGAAACAGC TTTCTGCACC AGTGGAGACT TCGTGTCCCC AGGGCCTTCC CCGTATCACA
2281GTCACGTGCA GTGCGGCCGC TTCAGGGAGA TGCTTCGCTG GTTTCTGGTG GATGTGGAGC
2341AGACTGCAGC TGGCCAGCCG CATCGCCAGT CTGCTGCTGG CCCTGCCATC ACCTGGGCCC
2401CAGCCATTGC CTACCCCTCC CCAGAGTGTG CTCGTTGCTG TGTTGGCTGC TCCTAGAACC
2461CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2521TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2581ATATATCTTG TGGAAAGGAC GAATCGTTGC GGCTGACGAC GATGATACGC GTTAAGTCga
2641caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)

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