Construct: ORF TRCN0000487806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020714.1_s317c1
- DNA Barcode:
- ATCGTTGCGGCTGACGACGATGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BRD4 (23476)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487806
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23476 | BRD4 | bromodomain containing 4 | NM_001330384.2 | 100% | 100% | |
| 2 | human | 23476 | BRD4 | bromodomain containing 4 | NM_014299.2 | 90.8% | 90.8% | 2159_2159delGinsCT;2161C>T;2166_2166delCins216 |
| 3 | human | 23476 | BRD4 | bromodomain containing 4 | XM_011527856.3 | 90.8% | 90.8% | 2159_2159delGinsCT;2161C>T;2166_2166delCins216 |
| 4 | human | 23476 | BRD4 | bromodomain containing 4 | NM_058243.2 | 56.5% | 52.2% | (many diffs) |
| 5 | human | 23476 | BRD4 | bromodomain containing 4 | XM_011527854.2 | 56.5% | 52.2% | (many diffs) |
| 6 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_198094.2 | 81.5% | 87.4% | (many diffs) |
| 7 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524695.3 | 81.4% | 87.3% | (many diffs) |
| 8 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524691.1 | 50.6% | 50.1% | (many diffs) |
| 9 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524690.2 | 50.6% | 50.1% | (many diffs) |
| 10 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_020508.4 | 49.4% | 48.9% | (many diffs) |
| 11 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524688.1 | 49.4% | 48.9% | (many diffs) |
| 12 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524689.2 | 49.4% | 48.9% | (many diffs) |
| 13 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_001286630.1 | 49.4% | 48.9% | (many diffs) |
| 14 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524683.2 | 49.4% | 48.9% | (many diffs) |
| 15 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524684.2 | 49.4% | 48.9% | (many diffs) |
| 16 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524685.1 | 49.4% | 48.9% | (many diffs) |
| 17 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524686.1 | 49.4% | 48.9% | (many diffs) |
| 18 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524687.2 | 49.4% | 48.9% | (many diffs) |
| 19 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524693.2 | 39.4% | 38.7% | (many diffs) |
| 20 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_011246543.2 | 39.4% | 38.7% | (many diffs) |
| 21 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_017317588.1 | 39.4% | 38.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2454
- ORF length:
- 2382
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctgcg gagagcggcc ctgggacgag attgagaaat ctgccagtaa 121 tgggggatgg actagaaact tcccaaatgt ctacaacaca ggcccaggcc caaccccagc 181 cagccaacgc agccagcacc aaccccccgc ccccagagac ctccaaccct aacaagccca 241 agaggcagac caaccaactg caatacctgc tcagagtggt gctcaagaca ctatggaaac 301 accagtttgc atggcctttc cagcagcctg tggatgccgt caagctgaac ctccctgatt 361 actataagat cattaaaacg cctatggata tgggaacaat aaagaagcgc ttggaaaaca 421 actattactg gaatgctcag gaatgtatcc aggacttcaa cactatgttt acaaattgtt 481 acatctacaa caagcctgga gatgacatag tcttaatggc agaagctctg gaaaagctct 541 tcttgcaaaa aataaatgag ctacccacag aagaaaccga gatcatgata gtccaggcaa 601 aaggaagagg acgtgggagg aaagaaacag ggacagcaaa acctggcgtt tccacggtac 661 caaacacaac tcaagcatcg actcctccgc agacccagac ccctcagccg aatcctcctc 721 ctgtgcaggc cacgcctcac cccttccctg ccgtcacccc ggacctcatc gtccagaccc 781 ctgtcatgac agtggtgcct ccccagccac tgcagacgcc cccgccagtg cccccccagc 841 cacaaccccc acccgctcca gctccccagc ccgtacagag ccacccaccc atcatcgcgg 901 ccaccccaca gcctgtgaag acaaagaagg gagtgaagag gaaagcagac accaccaccc 961 ccaccaccat tgaccccatt cacgagccac cctcgctgcc cccggagccc aagaccacca 1021 agctgggcca gcggcgggag agcagccggc ctgtgaaacc tccaaagaag gacgtgcccg 1081 actctcagca gcacccagca ccagagaaga gcagcaaggt ctcggagcag ctcaagtgct 1141 gcagcggcat cctcaaggag atgtttgcca agaagcacgc cgcctacgcc tggcccttct 1201 acaagcctgt ggacgtggag gcactgggcc tacacgacta ctgtgacatc atcaagcacc 1261 ccatggacat gagcacaatc aagtctaaac tggaggcccg tgagtaccgt gatgctcagg 1321 agtttggtgc tgacgtccga ttgatgttct ccaactgcta taagtacaac cctcctgacc 1381 atgaggtggt ggccatggcc cgcaagctcc aggatgtgtt cgaaatgcgc tttgccaaga 1441 tgccggacga gcctgaggag ccagtggtgg ccgtgtcctc cccggcagtg ccccctccca 1501 ccaaggttgt ggccccgccc tcatccagcg acagcagcag cgatagctcc tcggacagtg 1561 acagttcgac tgatgactct gaggaggagc gagcccagcg gctggctgag ctccaggagc 1621 agctcaaagc cgtgcacgag cagcttgcag ccctctctca gccccagcag aacaaaccaa 1681 agaaaaagga gaaagacaag aaggaaaaga aaaaagaaaa gcacaaaagg aaagaggaag 1741 tggaagagaa taaaaaaagc aaagccaagg aacctcctcc taaaaagacg aagaaaaata 1801 atagcagcaa cagcaatgtg agcaagaagg agccagcgcc catgaagagc aagccccctc 1861 ccacgtatga gtcggaggaa gaggacaagt gcaagcctat gtcctatgag gagaagcggc 1921 agctcagctt ggacatcaac aagctccccg gcgagaagct gggccgcgtg gtgcacatca 1981 tccagtcacg ggagccctcc ctgaagaatt ccaaccccga cgagattgaa atcgactttg 2041 agaccctgaa gccgtccaca ctgcgtgagc tggagcgcta tgtcacctcc tgtttgcgga 2101 agaaaaggaa acctcaagct gagaaagttg atgtgattgc cggctcctcc aagatgaagg 2161 gcttctcgtc ctCAGAGTCG GAGAGCTCCA GTGAGTCCAG CTCCTCTGAC AGCGAAGACT 2221 CCGAAACAGC TTTCTGCACC AGTGGAGACT TCGTGTCCCC AGGGCCTTCC CCGTATCACA 2281 GTCACGTGCA GTGCGGCCGC TTCAGGGAGA TGCTTCGCTG GTTTCTGGTG GATGTGGAGC 2341 AGACTGCAGC TGGCCAGCCG CATCGCCAGT CTGCTGCTGG CCCTGCCATC ACCTGGGCCC 2401 CAGCCATTGC CTACCCCTCC CCAGAGTGTG CTCGTTGCTG TGTTGGCTGC TCCTAGAACC 2461 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2521 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2581 ATATATCTTG TGGAAAGGAC GAATCGTTGC GGCTGACGAC GATGATACGC GTTAAGTCga 2641 caatcaacct ctggattaca aaatttgtga aagatt