Construct: ORF TRCN0000487873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020393.1_s317c1
- DNA Barcode:
- CCTTACGAGACCCGGGACGTAGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDPK1 (5170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | NM_002613.5 | 100% | 100% | |
| 2 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_024450296.1 | 95.1% | 95.1% | 0_1ins81 |
| 3 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023273.2 | 94.1% | 90.1% | (many diffs) |
| 4 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_011522521.3 | 93.9% | 92.8% | (many diffs) |
| 5 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023272.2 | 92.7% | 88.8% | (many diffs) |
| 6 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023274.1 | 89.4% | 85.4% | (many diffs) |
| 7 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_024450295.1 | 89.4% | 85.4% | (many diffs) |
| 8 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023271.2 | 88.6% | 83.8% | (many diffs) |
| 9 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | NM_001261816.2 | 81.1% | 80.5% | (many diffs) |
| 10 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | NM_031268.6 | 77.1% | 76.9% | 328_329ins381 |
| 11 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_011522523.3 | 72.4% | 71.1% | (many diffs) |
| 12 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023276.1 | 72% | 68% | (many diffs) |
| 13 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023275.2 | 67.7% | 62.9% | (many diffs) |
| 14 | human | 5170 | PDPK1 | 3-phosphoinositide dependen... | XM_017023277.1 | 56.6% | 52.8% | (many diffs) |
| 15 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | NM_011062.4 | 87.8% | 94.6% | (many diffs) |
| 16 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | NM_001286662.1 | 83.2% | 89.8% | (many diffs) |
| 17 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317320.1 | 83.2% | 89.8% | (many diffs) |
| 18 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317321.1 | 83.2% | 89.8% | (many diffs) |
| 19 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317322.1 | 83.2% | 89.8% | (many diffs) |
| 20 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317323.1 | 83.2% | 89.8% | (many diffs) |
| 21 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317326.1 | 83.2% | 89.8% | (many diffs) |
| 22 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | NM_001080773.2 | 82.1% | 88.1% | (many diffs) |
| 23 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317324.1 | 62.3% | 61.1% | (many diffs) |
| 24 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317325.1 | 62.3% | 61.1% | (many diffs) |
| 25 | mouse | 18607 | Pdpk1 | 3-phosphoinositide dependen... | XM_017317327.1 | 54.1% | 59.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1740
- ORF length:
- 1668
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccagg accaccagcc agctgtatga cgccgtgccc atccagtcca 121 gcgtggtgtt atgttcctgc ccatccccat caatggtgag gacccagact gagtccagca 181 cgccccctgg cattcctggt ggcagcaggc agggccccgc catggacggc actgcagccg 241 agcctcggcc cggcgccggc tccctgcagc atgcccagcc tccgccgcag cctcggaaga 301 agcggcctga ggacttcaag tttgggaaaa tccttgggga aggctctttt tccacggttg 361 tcctggctcg agaactggca acctccagag aatatgcgat taaaattctg gagaagcgac 421 atatcataaa agagaacaag gtcccctatg taaccagaga gcgggatgtc atgtcgcgcc 481 tggatcaccc cttctttgtt aagctttact tcacatttca ggacgacgag aagctgtatt 541 tcggccttag ttatgccaaa aatggagaac tacttaaata tattcgcaaa atcggttcat 601 tcgatgagac ctgtacccga ttttacacgg ctgagattgt gtctgcttta gagtacttgc 661 acggcaaggg catcattcac agggacctta aaccggaaaa cattttgtta aatgaagata 721 tgcacatcca gatcacagat tttggaacag caaaagtctt atccccagag agcaaacaag 781 ccagggccaa ctcattcgtg ggaacagcgc agtacgtttc tccagagctg ctcacggaga 841 agtccgcctg taagagttca gacctttggg ctcttggatg cataatatac cagcttgtgg 901 caggactccc accattccga gctggaaacg agtatcttat atttcagaag atcattaagt 961 tggaatatga ctttccagaa aaattcttcc ctaaggcaag agacctcgtg gagaaacttt 1021 tggttttaga tgccacaaag cggttaggct gtgaggaaat ggaaggatac ggacctctta 1081 aagcacaccc gttcttcgag tccgtcacgt gggagaacct gcaccagcag acgcctccga 1141 agctcaccgc ttacctgccg gctatgtcgg aagacgacga ggactgctat ggcaattatg 1201 acaatctcct gagccagttt ggctgcatgc aggtgtcttc gtcctcctcc tcacactccc 1261 tgtcagcctc cgacacgggc ctgccccaga ggtcaggcag caacatagag cagtacattc 1321 acgatctgga ctcgaactcc tttgaactgg acttacagtt ttccgaagat gagaagaggt 1381 tgttgttgga gaagcaggct ggcggaaacc cttggcacca gtttgtagaa aataatttaa 1441 tactaaagat gggcccagtG GATAAGCGGA AGGGTTTATT TGCAAGACGA CGACAGCTGT 1501 TGCTCACAGA AGGACCACAT TTATATTATG TGGATCCTGT CAACAAAGTT CTGAAAGGTG 1561 AAATTCCTTG GTCACAAGAA CTTCGACCAG AGGCCAAGAA TTTTAAAACT TTCTTTGTCC 1621 ACACGCCTAA CAGGACGTAT TATCTGATGG ACCCCAGCGG GAACGCACAC AAGTGGTGCA 1681 GGAAGATCCA GGAGGTTTGG AGGCAGCGAT ACCAGAGCCA CCCGGACGCC GCTGTGCAGT 1741 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1801 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1861 GGCTTTATAT ATCTTGTGGA AAGGACGACC TTACGAGACC CGGGACGTAG GTACGCGTTA 1921 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt